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User:HassanForoughiAsl/29 June 2010
From 2010.igem.org
(New page: ''"It has become dangerously acceptable to conclude that proteins interact directly provided that (i) the interaction is biologically plausible; (ii) the proteins are co-expressed; and (ii...) |
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| - | ''"It has become dangerously acceptable to conclude that proteins interact directly provided that (i) the interaction is biologically plausible; (ii) the proteins are co-expressed; and (iii) an interaction is demonstrated by glutathione-S-transferase (GST)-pulldowns and/or co-immunoprecipitations (co-IPs)"... "The most obvious caveat with co-IP data is that a positive result does not imply a direct interaction between two proteins – binding could be mediated by other partners."..."" | + | ''"It has become dangerously acceptable to conclude that proteins interact directly provided that (i) the interaction is biologically plausible; (ii) the proteins are co-expressed; and (iii) an interaction is demonstrated by glutathione-S-transferase (GST)-pulldowns and/or co-immunoprecipitations (co-IPs)"... "The most obvious caveat with co-IP data is that a positive result does not imply a direct interaction between two proteins – binding could be mediated by other partners."..."In addition, a widespread misconception is that co-IPs from extracts provide in vivo evidence for the existence of an interaction. This is not accurate, particularly when the experiment is carried out using overexpressed proteins in cell lines."..."" |
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"Protein interactions: is seeing believing? J. P. Mackay et al. 2007" | "Protein interactions: is seeing believing? J. P. Mackay et al. 2007" | ||
Latest revision as of 13:01, 29 June 2010
"It has become dangerously acceptable to conclude that proteins interact directly provided that (i) the interaction is biologically plausible; (ii) the proteins are co-expressed; and (iii) an interaction is demonstrated by glutathione-S-transferase (GST)-pulldowns and/or co-immunoprecipitations (co-IPs)"... "The most obvious caveat with co-IP data is that a positive result does not imply a direct interaction between two proteins – binding could be mediated by other partners."..."In addition, a widespread misconception is that co-IPs from extracts provide in vivo evidence for the existence of an interaction. This is not accurate, particularly when the experiment is carried out using overexpressed proteins in cell lines."...""
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"Protein interactions: is seeing believing? J. P. Mackay et al. 2007"
