Team:Stockholm/Modelling/Introduction

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=== Introduction ===
=== Introduction ===
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As a future work of our project the aim is to manipulate the bacteria to produce in environments outside laboratory as a bacteria therapy. Bacteria will produce proteins fusion with cpp (cell penetration peptides) while it is on skin. Two main factors need to be considered here:
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<div align="justify">As a future work of our project the aim is to manipulate the bacteria to reproduce in environments outside the laboratory for bacterial therapy. Bacteria will express proteins fused to cell-penetrating peptides (CPPs) while growing on skin. Since bacteria will be outside normal lab environment, we need to choose proper assumptions to fit the situation.
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* Since bacteria will be outside of lab environment, we need to choose proper assumptions to fit the situation.
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* CPP can lead to the bacteria's death at certain concentrations, as they will penetrate the membrane and eventually kill bacteria. So it is crucial to have the bacteria to begin the production when it comes with contact with skin.
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We have LacI as repressor, so idea is to have a creme on skin which contains lactose as inducer. As a matter of fact good timing is needed before CPP reaches a critical concentration to kill bacteria. It seems in this scenario we need to take into account possible details such as degradation rates, cell growth, and factors that will effect a stable production such as translational and transcriptional delay.
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In our pEX expression vector we have ''lacI'' as a repressor of the P<sub>tac</sub> promoter, so the idea is to have a cream on the skin which contains lactose as an inducer for P<sub>tac</sub>. It seems in this scenario we need to take into account possible details such as degradation rates, cell growth, and factors that will affect stable production such as translational and transcriptional delay. Therefore, we are aiming to achieve a predicting model for production of protein in our system of bacteria and have the model to describe how production is induced in bacteria from the time induction starts. In this model we will not include or model or try to predict re-pigmentation, because of high complications in target human cells. For further reading about mathematics one can look in this article: [http://www.biomedcentral.com/1752-0509/3/60 The mathematics of tanning J. et al. 2008].
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Therefore, we are aiming to achieve a predicting model for production of protein in our system of bacteria and have the model to describe production is induced in bacteria from the time induction starts until it is dead because of CPPs penetrating bacteria membrane. In this model we will not include or model or try to predict re-pigmentation, because of high complications in target human cells. for further reading about mathematics one can [http://www.biomedcentral.com/1752-0509/3/60| The mathematics of tanning Thingnes J. et al. 2008]
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Out of these details, there are some data we need to know in advance. These details include:
Out of these details, there are some data we need to know in advance. These details include:
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* How much lactose is available for bacteria? i. e. Concentration of Lactose on skin
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:* How much lactose is available for bacteria? i. e. Concentration of lactose on the skin
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* Production rate for biomolecules in host bacteria
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:* Production rate for biomolecules in host bacteria
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* Allolactose/Lactose degradation rate
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:* Allolactose/lactose degradation rate
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* Allolactose/Lactose absorption through skin
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:* Allolactose/lactose absorption through skin
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* Critical concentration of CPP
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Latest revision as of 23:39, 26 October 2010

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Introduction

As a future work of our project the aim is to manipulate the bacteria to reproduce in environments outside the laboratory for bacterial therapy. Bacteria will express proteins fused to cell-penetrating peptides (CPPs) while growing on skin. Since bacteria will be outside normal lab environment, we need to choose proper assumptions to fit the situation.

In our pEX expression vector we have lacI as a repressor of the Ptac promoter, so the idea is to have a cream on the skin which contains lactose as an inducer for Ptac. It seems in this scenario we need to take into account possible details such as degradation rates, cell growth, and factors that will affect stable production such as translational and transcriptional delay. Therefore, we are aiming to achieve a predicting model for production of protein in our system of bacteria and have the model to describe how production is induced in bacteria from the time induction starts. In this model we will not include or model or try to predict re-pigmentation, because of high complications in target human cells. For further reading about mathematics one can look in this article: [http://www.biomedcentral.com/1752-0509/3/60 The mathematics of tanning J. et al. 2008].

Out of these details, there are some data we need to know in advance. These details include:

  • How much lactose is available for bacteria? i. e. Concentration of lactose on the skin
  • Production rate for biomolecules in host bacteria
  • Allolactose/lactose degradation rate
  • Allolactose/lactose absorption through skin





The Faculty of Science at Stockholm University Swedish Vitiligo association (Svenska Vitiligoförbundet) Geneious Fermentas/ Sigma-Aldrich/

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