Team:Berkeley

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[https://2010.igem.org/Team:Berkeley Home] &nbsp;
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[https://2010.igem.org/Team:Berkeley/Project_Overview Project Overview] &nbsp;
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[https://2010.igem.org/Team:Berkeley/Choanoflagellates Choanoflagellates] &nbsp;
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[https://2010.igem.org/Team:Berkeley/Parts Parts] &nbsp;
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[https://2010.igem.org/Team:Berkeley/Clotho Clotho] &nbsp;
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[https://2010.igem.org/Team:Berkeley/Human_Practices Human Practices] &nbsp;
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[https://2010.igem.org/Team:Berkeley/Notebooks Group Members]
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<center><font size="5" face="Comic Sans">Our Team</font></center> <br>
 
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<center><font size="3" face="Comic Sans">Photo by Peg Skorpinski</font></center>
 
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<font size="5" face="Comic Sans">Acknowledgements</font> <br>
 
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We thank our wonderful advisors: Chris Anderson, Terry Johnson, Tim Hsiau, Jin Huh for their guidance and support. We also thank our generous sponsors:<br>
 
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<center>(Pronounced like "Cocoa")</center>
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Single-celled phagocytic eukaryotes, such as Choanoflagellates, are of great interest to developmental biologists because they may be the last living immediate precursor on the evolutionary tree to animals. These easy-to-culture and robust organisms are also a desirable low-cost eukaryotic chassis for synthetic biology, but there are few to no tools for delivering biomolecules into these organisms. We engineered E. coli to deliver proteins and/or DNA payloads into these bacteria-devouring eukaryotes. Once ingested, our E. coli are programmed to self-lyse and porate the phagosome, releasing their payloads into the cytosol. This delivery mechanism has the potential to deliver payload to any phagocytic organism with a cholesterol-based membrane. As part of our parallel software effort to rework the Clotho plugin environment and API, we made automatic biosafety handling an intrinsic feature of the core. Together, these tools provide a foundation for metazoan synthetic biology and a framework for improving safety in our field.
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===Sponsors===
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<a href="http://www.quintarabio.com/">
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&nbsp;&nbsp;&nbsp;
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<a href="http://usa.autodesk.com/">
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&nbsp;&nbsp;&nbsp;
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<a href="http://www.synberc.org/">
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&nbsp;&nbsp;&nbsp;
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<a href="http://www.yaliscafe.com/">
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We would like to thank our sponsors, shown above, and our wonderful advisors Chris Anderson, Terry Johnson, Tim Hsiau, and Jin Huh for their guidance and support.

Latest revision as of 23:43, 27 October 2010

Plain header.png




ChoaChoa delivery header.png
(Pronounced like "Cocoa")

Single-celled phagocytic eukaryotes, such as Choanoflagellates, are of great interest to developmental biologists because they may be the last living immediate precursor on the evolutionary tree to animals. These easy-to-culture and robust organisms are also a desirable low-cost eukaryotic chassis for synthetic biology, but there are few to no tools for delivering biomolecules into these organisms. We engineered E. coli to deliver proteins and/or DNA payloads into these bacteria-devouring eukaryotes. Once ingested, our E. coli are programmed to self-lyse and porate the phagosome, releasing their payloads into the cytosol. This delivery mechanism has the potential to deliver payload to any phagocytic organism with a cholesterol-based membrane. As part of our parallel software effort to rework the Clotho plugin environment and API, we made automatic biosafety handling an intrinsic feature of the core. Together, these tools provide a foundation for metazoan synthetic biology and a framework for improving safety in our field.


Sponsors


               
 

We would like to thank our sponsors, shown above, and our wonderful advisors Chris Anderson, Terry Johnson, Tim Hsiau, and Jin Huh for their guidance and support.

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