Team:Groningen/18 October 2010

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Write wiki text for killswitch and gene expression model. Create all figures, tables and equations in wiki format for gene expression model and killswitch model. ''Laura''
Write wiki text for killswitch and gene expression model. Create all figures, tables and equations in wiki format for gene expression model and killswitch model. ''Laura''
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'''David'''
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<br>
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'''Peter en David'''
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Expression experiment contruct E&H chaplin and C chaplin. Growing in batches of 0.5 L liquid TY medium at 37 degrees. Inoculation with overnight culture with a starting OD of 0.1. Inducing at an OD of 0.5 with 1.5% subtilin overnight. Harvesting the overnight culture and washing pellet before disrupting it.
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THT measurement
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<pre>
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[[Image:C CN EH EHN gr.jpg]]
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The drag coefficient c_\mathrm d\, is defined as:
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<br>
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Mass spec malditov results
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    c_\mathrm d = \dfrac{2 F_\mathrm d}{\rho v^2 A}\, ,
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<br>
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[[Image:massspec malditov disruption]]
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where:
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    F_\mathrm d\, is the drag force, which is by definition the force component in the direction of the flow velocity,[6]
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    \rho\, is the mass density of the fluid,[7]
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    v is the speed of the object relative to the fluid, and
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    A\, is the reference area.
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The reference area depends on what type of drag coefficient is being measured. For automobiles and many other objects, the reference area is the projected frontal area of the vehicle. This may not necessarily be the cross sectional area of the vehicle, depending on where the cross section is taken. For example, for a sphere A = \pi r^2\, (note this is not the surface area = \!\ 4 \pi r^2).
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<br>
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'''David'''
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Speed
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measurements in Bremen on surface activity of chaplin coated objects
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0 0 snelheid = 0,016 *frq + -0,0556
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3 0,01 0,01 0,01
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6 0,05 0,05 0,05
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9 0,09 0,09 0,09
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12 0,13 0,13 0,13
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15 0,17 0,18 0,175
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18 0,22 0,22 0,22
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21 0,27 0,28 0,275
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24 0,31 0,32 0,315
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27 0,37 0,38 0,375
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30 0,42 0,43 0,425
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33 0,47 0,48 0,475
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36 0,52 0,53 0,525
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39 0,57 0,57 0,57
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42 0,62 0,63 0,625
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45 0,66 0,67 0,665
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48 0,71 0,72 0,715
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<br>
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We wanted to know if there was an effect of our chaplin proteins on surface drag in the water. To test this we wanted to measure the water resistance of a spindel shaped object in the water at different speeds. First do the measurements with a clean spindel and secondly after coating the spindel with purified chaplins from streptomyces. The spindle that was used for the mesurements, this shapes is designed minimize the effect of shape of the objects drag. Thereby maximizing the effect of surface resistance on the drag coeffient.
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freq distance blanco freq Re distance distance corr drag coeff force dichtheid lengte/doorsnede viscositeit snelheid A relatie afstand kracht oppervlakte
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0 -0,00004 12 1226 0,00041 0,00045 11,33089299 999 0,009 0,001 0,1364 23,4 0,0001
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[[Image:pinnacleunitvandennis.jpg]]
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0 -0,00019 24 2953 0,00195 0,00214 9,295822904 0,3284
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0 -0,00019 36 4679 0,0043 0,00449 7,766978985 0,5204
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0 0,00046 48 6405 0,00856 0,0081 7,476830962 0,7124
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freq distance blanco freq Re distance distance corr drag coeff force dichtheid lengte/doorsnede viscositeit snelheid
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0 -0,00028 12 1226 0,00017 0,00045 11,33089299 999 0,009 0,001 0,1364
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0 -0,00016 24 2953 0,00202 0,00218 9,469576603 0,3284
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0 -0,00026 36 4679 0,00459 0,00485 8,389721175 0,5204
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0 -0,00026 48 6405 0,00856 0,00882 8,141438159 0,7124
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freq distance blanco freq Re distance distance corr drag coeff force dichtheid lengte/doorsnede viscositeit snelheid
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0 -0,00037 12 1226 0,00012 0,00049 12,33808348 999 0,009 0,001 0,1364
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0 -0,00043 24 2953 0,00167 0,0021 9,122069204 0,3284
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0 -0,00028 36 4679 0,00468 0,00496 8,580003511 0,5204
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0 -0,00037 48 6405 0,00844 0,00881 8,132207504 0,7124
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gemiddelde
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freq distance blanco freq Re distance distance corr drag coeff force dichtheid lengte/doorsnede viscositeit snelheid
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0 -0,00023 12 1226 0,00012 0,000463333 11,66662315 999 0,009 0,001 0,1364
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0 -0,00026 24 2953 0,00167 0,00214 9,295822904 0,3284
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0 -0,000243333 36 4679 0,00468 0,004766667 8,24556789 0,5204
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0 -5,66667E-05 48 6405 0,00844 0,008576667 7,916825542 0,7124
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SD
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0,000170587 0,000155027 2,3094E-05 0,581501699
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0,000147986 0,000185203 4E-05 0,173753699
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4,72582E-05 0,000198578 0,000245832 0,425249747
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0,000450814 6,9282E-05 0,000412836 0,381074433
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<br>
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</pre>
 

Latest revision as of 03:54, 28 October 2010

Modellers: Find constants and parameters for gene expression model, finish modelling and put everything on the wiki Laura, Djoke

Write wiki text for killswitch and gene expression model. Create all figures, tables and equations in wiki format for gene expression model and killswitch model. Laura


Peter en David

Expression experiment contruct E&H chaplin and C chaplin. Growing in batches of 0.5 L liquid TY medium at 37 degrees. Inoculation with overnight culture with a starting OD of 0.1. Inducing at an OD of 0.5 with 1.5% subtilin overnight. Harvesting the overnight culture and washing pellet before disrupting it.

THT measurement File:C CN EH EHN gr.jpg
Mass spec malditov results
File:Massspec malditov disruption

David

measurements in Bremen on surface activity of chaplin coated objects

We wanted to know if there was an effect of our chaplin proteins on surface drag in the water. To test this we wanted to measure the water resistance of a spindel shaped object in the water at different speeds. First do the measurements with a clean spindel and secondly after coating the spindel with purified chaplins from streptomyces. The spindle that was used for the mesurements, this shapes is designed minimize the effect of shape of the objects drag. Thereby maximizing the effect of surface resistance on the drag coeffient.


Pinnacleunitvandennis.jpg


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