Team:Groningen/Biobricks

From 2010.igem.org

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Here is our registry of our biobricks:
Here is our registry of our biobricks:
<groupparts>iGEM010 Groningen</groupparts>
<groupparts>iGEM010 Groningen</groupparts>
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'''Summary:'''
'''Summary:'''
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'''Chaplins'''
'''Chaplins'''
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BBa_K305001 Hydrophobic protein chaplin C (chpC)
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<partinfo>BBa_K305001</partinfo> Hydrophobic protein chaplin C (chpC)
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
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BBa_K305002 chaplin C (chpDC) with modified sortase binding motive
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<partinfo>BBa_K305002</partinfo> chaplin C (chpDC) with modified sortase binding motive
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.  
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.  
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Modified C-terminal Sortase(SrtA from S. aureus.) recognition site.     
Modified C-terminal Sortase(SrtA from S. aureus.) recognition site.     
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BBa_K305003 Hydrophobic protein chaplin E (chpE)  
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<partinfo>BBa_K305003</partinfo> Hydrophobic protein chaplin E (chpE)  
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
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BBa_K305004 Hydrophobic protein chaplin H (chpH)
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<partinfo>BBa_K305004</partinfo> Hydrophobic protein chaplin H (chpH)
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
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BBa_K305005Sortase srtA  
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<partinfo>BBa_K305005</partinfo> Sortase srtA  
Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall
Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall
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'''Promotors'''
'''Promotors'''
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BBa_K305006 yqxMpr  
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<partinfo>BBa_K305006</partinfo> yqxMpr  
yqxM promotor effective in biofilm formation, regulates tasA production
yqxM promotor effective in biofilm formation, regulates tasA production
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BBa_K305007 srfApr 1
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<partinfo>BBa_K305007</partinfo> srfApr 1
Promotor for the surfactin regulatory gene.
Promotor for the surfactin regulatory gene.
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BBa_K305008 srfApr 2
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<partinfo>BBa_K305008</partinfo> srfApr 2
Promotor for the surfactin regulatory gene.
Promotor for the surfactin regulatory gene.
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BBa_K305009 srfApr1>GFP   
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<partinfo>BBa_K305009</partinfo> srfApr1>GFP   
Promotor for the surfactin regulatory gene with GFP.
Promotor for the surfactin regulatory gene with GFP.
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BBa_K305010 srfApr2>GFP
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<partinfo>BBa_K305010</partinfo> srfApr2>GFP
Promotor for the surfactin regulatory gene with GFP.
Promotor for the surfactin regulatory gene with GFP.
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'''Plasmid Backbone'''
'''Plasmid Backbone'''
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BBa_K305011 Subtilin inducible expression plasmid for Bacillus subtilis
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<partinfo>BBa_K305011</partinfo> Subtilin inducible expression plasmid for Bacillus subtilis
Adapted from the system for subtilin-regulated gene expression (SURE) in Bacillus subtilis [1], this plasmid was  
Adapted from the system for subtilin-regulated gene expression (SURE) in Bacillus subtilis [1], this plasmid was  
constructed to express proteins present in RCF 10 BioBricks upon subtilin induction.
constructed to express proteins present in RCF 10 BioBricks upon subtilin induction.
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Latest revision as of 12:01, 26 October 2010

Biobricks

Here is our registry of our biobricks: <groupparts>iGEM010 Groningen</groupparts>


Summary:

Below is given a short description of all the biobricks in our registry.


Chaplins

<partinfo>BBa_K305001</partinfo> Hydrophobic protein chaplin C (chpC)

Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.

<partinfo>BBa_K305002</partinfo> chaplin C (chpDC) with modified sortase binding motive

Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.

Modified C-terminal Sortase(SrtA from S. aureus.) recognition site.

<partinfo>BBa_K305003</partinfo> Hydrophobic protein chaplin E (chpE)

Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.

<partinfo>BBa_K305004</partinfo> Hydrophobic protein chaplin H (chpH)

Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.

<partinfo>BBa_K305005</partinfo> Sortase srtA

Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall


Promotors

<partinfo>BBa_K305006</partinfo> yqxMpr

yqxM promotor effective in biofilm formation, regulates tasA production

<partinfo>BBa_K305007</partinfo> srfApr 1

Promotor for the surfactin regulatory gene.

<partinfo>BBa_K305008</partinfo> srfApr 2

Promotor for the surfactin regulatory gene.

<partinfo>BBa_K305009</partinfo> srfApr1>GFP

Promotor for the surfactin regulatory gene with GFP.

<partinfo>BBa_K305010</partinfo> srfApr2>GFP

Promotor for the surfactin regulatory gene with GFP.


Plasmid Backbone

<partinfo>BBa_K305011</partinfo> Subtilin inducible expression plasmid for Bacillus subtilis

Adapted from the system for subtilin-regulated gene expression (SURE) in Bacillus subtilis [1], this plasmid was constructed to express proteins present in RCF 10 BioBricks upon subtilin induction.






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