"
Team:Groningen/11 October 2010
From 2010.igem.org
(New page: '''Laura''' Debugging the matlab model for gene expression and killswitch. Find more information about constants used in the gene expression model.) |
|||
| (One intermediate revision not shown) | |||
| Line 2: | Line 2: | ||
Debugging the matlab model for gene expression and killswitch. Find more information about constants used in the gene expression model. | Debugging the matlab model for gene expression and killswitch. Find more information about constants used in the gene expression model. | ||
| + | |||
| + | '''Peter & David''' | ||
| + | <br> | ||
| + | Expression experiment on new transformants: E+H1, E+H2, C1, C2, E and H mix 4 clones. | ||
| + | Growing the strains from overnight cultures in 5 ml of TY medium at 37 degrees. | ||
| + | |||
| + | '''David''' | ||
| + | <br> | ||
| + | Biofilm paste experiment | ||
| + | <br> | ||
| + | Growing biofilms on sterilized ceramics that have been coated with athin layer of TY medium with corn starch. | ||
| + | <br> | ||
| + | Mix: 30 mg cornstarch/ml TY 1% agar medium and 0.5% glucose | ||
| + | |||
| + | After dissolving the agar and addition of corn starch teh pieces of cermics we added an taken out | ||
| + | |||
| + | [[Image:ceramic coating gr1.jpg]] | ||
| + | [[Image:ceramic coating gr1.jpg]] | ||
| + | [[Image:ceramic coating gr1.jpg]] | ||
| + | [[Image:ceramic coating gr1.jpg]] | ||
| + | |||
| + | |||
| + | Expression experiment: | ||
| + | |||
| + | [[Image:growthchaplineEN.jpg]] | ||
| + | |||
| + | ThT measurement of EH induced together with C non induced(this due to a mixup during he experiment), EH non induced, C induced | ||
| + | |||
| + | [[Image:disrupted last measurement gr.jpg]] | ||
Latest revision as of 02:02, 28 October 2010
Laura
Debugging the matlab model for gene expression and killswitch. Find more information about constants used in the gene expression model.
Peter & David
Expression experiment on new transformants: E+H1, E+H2, C1, C2, E and H mix 4 clones.
Growing the strains from overnight cultures in 5 ml of TY medium at 37 degrees.
David
Biofilm paste experiment
Growing biofilms on sterilized ceramics that have been coated with athin layer of TY medium with corn starch.
Mix: 30 mg cornstarch/ml TY 1% agar medium and 0.5% glucose
After dissolving the agar and addition of corn starch teh pieces of cermics we added an taken out
Expression experiment:
ThT measurement of EH induced together with C non induced(this due to a mixup during he experiment), EH non induced, C induced
