Team:Yale/Our Project/Protocols
From 2010.igem.org
(Difference between revisions)
(5 intermediate revisions not shown) | |||
Line 11: | Line 11: | ||
<ul id="proj-nav"> | <ul id="proj-nav"> | ||
<li><a href="https://2010.igem.org/Team:Yale/Our Project">introduction</a></li> | <li><a href="https://2010.igem.org/Team:Yale/Our Project">introduction</a></li> | ||
+ | <li><a href="https://2010.igem.org/Team:Yale/Our Project/Applications">applications</a></li> | ||
<li><a href="https://2010.igem.org/Team:Yale/Our Project/Methods">methods</a></li> | <li><a href="https://2010.igem.org/Team:Yale/Our Project/Methods">methods</a></li> | ||
<li><a href="https://2010.igem.org/Team:Yale/Our Project/Notebook">lab notebook</a></li> | <li><a href="https://2010.igem.org/Team:Yale/Our Project/Notebook">lab notebook</a></li> | ||
Line 16: | Line 17: | ||
<li><a href="https://2010.igem.org/Team:Yale/Our Project/Safety">safety</a></li> | <li><a href="https://2010.igem.org/Team:Yale/Our Project/Safety">safety</a></li> | ||
<li><a href="https://2010.igem.org/Team:Yale/Our Project/Future Work">future work</a></li> | <li><a href="https://2010.igem.org/Team:Yale/Our Project/Future Work">future work</a></li> | ||
- | |||
</ul> | </ul> | ||
</p> | </p> | ||
Line 36: | Line 36: | ||
<a id="link" href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/gel_extract">gel extraction</a> <br/> | <a id="link" href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/gel_extract">gel extraction</a> <br/> | ||
<a id="link" href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/PCR_purify">PCR purification</a> <br/> | <a id="link" href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/PCR_purify">PCR purification</a> <br/> | ||
- | <a id="link" href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/EtOH_precipitate">ethanol | + | <a id="link" href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/EtOH_precipitate">ethanol precipitation</a> |
<h4> Recipes </h4> | <h4> Recipes </h4> | ||
- | <a id="link" href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/ | + | <a id="link" href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/minimal_medium">MOPS minimal medium</a> <br/> |
<a id="link" href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/SOC_broth">SOC medium</a> <br/> | <a id="link" href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/SOC_broth">SOC medium</a> <br/> | ||
<a id="link" href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/LB_agar">LB agar</a> <br/> | <a id="link" href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/LB_agar">LB agar</a> <br/> | ||
- | <a id="link" href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/TSI_agar">Triple Sugar Iron (TSI) agar</a> | + | <a id="link" href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/TSI_agar">Triple Sugar Iron (TSI) agar</a> <br/> |
+ | <a id="link" href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/bacteria mixture for H2S Assay">Bacteria Mixture for H<sub>2</sub>S Assay</a><br/> | ||
<h4> Copper absorbance protocols </h4> | <h4> Copper absorbance protocols </h4> |
Latest revision as of 19:02, 27 October 2010
our project
protocols
Bacterial protocols
transformationDNA protocols
miniprepgel extraction
PCR purification
ethanol precipitation
Recipes
MOPS minimal mediumSOC medium
LB agar
Triple Sugar Iron (TSI) agar
Bacteria Mixture for H2S Assay
Copper absorbance protocols
standard absorbance curve measurementcopper disappearance as a function of bacterial growth curve