Team:Yale/Our Project/Protocols

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<ul id="proj-nav">
<ul id="proj-nav">
<li><a href="https://2010.igem.org/Team:Yale/Our Project">introduction</a></li>
<li><a href="https://2010.igem.org/Team:Yale/Our Project">introduction</a></li>
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<li><a href="https://2010.igem.org/Team:Yale/Our Project/Applications">applications</a></li>
<li><a href="https://2010.igem.org/Team:Yale/Our Project/Methods">methods</a></li>
<li><a href="https://2010.igem.org/Team:Yale/Our Project/Methods">methods</a></li>
<li><a href="https://2010.igem.org/Team:Yale/Our Project/Notebook">lab notebook</a></li>
<li><a href="https://2010.igem.org/Team:Yale/Our Project/Notebook">lab notebook</a></li>
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<li><a href="https://2010.igem.org/Team:Yale/Our Project/Safety">safety</a></li>
<li><a href="https://2010.igem.org/Team:Yale/Our Project/Safety">safety</a></li>
<li><a href="https://2010.igem.org/Team:Yale/Our Project/Future Work">future work</a></li>
<li><a href="https://2010.igem.org/Team:Yale/Our Project/Future Work">future work</a></li>
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<li><a href="https://2010.igem.org/Team:Yale/Our Project/Applications">applications</a></li>
 
</ul>
</ul>
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</p>
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<a id="link" href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/gel_extract">gel extraction</a> <br/>
<a id="link" href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/gel_extract">gel extraction</a> <br/>
<a id="link" href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/PCR_purify">PCR purification</a> <br/>
<a id="link" href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/PCR_purify">PCR purification</a> <br/>
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<a id="link" href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/EtOH_precipitate">ethanol purification</a>  
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<a id="link" href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/EtOH_precipitate">ethanol precipitation</a>  
<h4> Recipes </h4>  
<h4> Recipes </h4>  
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<a id="link" href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/minimal_media">MOPS minimal media</a> <br/>
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<a id="link" href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/minimal_medium">MOPS minimal medium</a> <br/>
<a id="link" href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/SOC_broth">SOC medium</a> <br/>
<a id="link" href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/SOC_broth">SOC medium</a> <br/>
<a id="link" href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/LB_agar">LB agar</a>  <br/>
<a id="link" href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/LB_agar">LB agar</a>  <br/>
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<a id="link" href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/TSI_agar">Triple Sugar Iron (TSI) agar</a>  
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<a id="link" href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/TSI_agar">Triple Sugar Iron (TSI) agar</a> <br/>
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<a id="link" href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/bacteria mixture for H2S Assay">Bacteria Mixture for H<sub>2</sub>S Assay</a><br/>
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<br /> The composition of the plates were:<br /> 
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<br /><li>500 mL water</a></li>
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<li>1.5 g Meat Extract </a></li>
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<li>1.5 g Yeast Extract</a></li>
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<li>7.5 g Pancreatic Digest of Casein</a></li>
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<li> 2.5 g Protease Peptone</a></li>
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<li>0.5 g Dextrose</a></li>
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<li>0.5 g Lactose</a></li>
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<li>0.5 g Sucrose</a></li>
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<li>0.26 g Ferrous Ammonium Sulfate (hexahydrate)</a></li>
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<li>2.5 g NaCl</a></li>
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<li>0.15 g Sodium Thiosulfate</a></li>
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<li>6 g Agar</a></li>
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<li>12 mg Phenol Red</a></li>
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<li>+ Ampicillin</a></li>
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<h4> Copper absorbance protocols </h4>
<h4> Copper absorbance protocols </h4>

Latest revision as of 19:02, 27 October 2010

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