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Team:Harvard/vectors/parts
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<h1>parts and primers</h1> | <h1>parts and primers</h1> | ||
<h2>vectors</h2> | <h2>vectors</h2> | ||
| - | For more information on the vectors visit our "<a href=" | + | For more information on the vectors visit our "<a href="https://2010.igem.org/Team:Harvard/vectors/vectors">meet the vectors</a>" page<br /><br /> |
<table cellspacing="0"> | <table cellspacing="0"> | ||
| - | <tr><td><b>Name</b></td><td><b>Original Name</b></td><td><b>Description</b></td><td><b>Resistance</b></td><td><b>Resistance (Plants)</b></td><td><b>Sequence</b></td></tr> | + | <tr><td><b>Name</b></td><td><b>Biobrick Name</b></td><td><b>Original Name</b></td><td><b>Description</b></td><td><b>Resistance</b></td><td><b>Resistance (Plants)</b></td><td><b>Sequence</b></td></tr> |
| - | <tr><td>V1</td><td>pORE Open Series 1 (O1)</td><td>Agrobacterium vector open series</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/3/37/PORE_O1.gb">link</a></td></tr> | + | <tr><td>V1</td><td><center>-</center></td><td>pORE Open Series 1 (O1)</td><td>Agrobacterium vector open series</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/3/37/PORE_O1.gb">link</a></td></tr> |
| - | <tr><td>V2</td><td>pORE Open Series 2 (O2)</td><td>Agrobacterium vector open series</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/f/fc/PORE_O2.gb">link</a></td></tr> | + | <tr><td>V2</td><td><center>-</center></td><td>pORE Open Series 2 (O2)</td><td>Agrobacterium vector open series</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/f/fc/PORE_O2.gb">link</a></td></tr> |
| - | <tr><td>V3</td><td>pORE Expression Series 3 (E3)</td><td>Agrobacterium vector with ENTCUP2 promoter</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/e/e7/PORE_E3.gb">link</a></td></tr> | + | <tr><td>V3</td><td><center>-</center></td><td>pORE Expression Series 3 (E3)</td><td>Agrobacterium vector with ENTCUP2 promoter</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/e/e7/PORE_E3.gb">link</a></td></tr> |
| - | <tr><td>V4</td><td>pORE Expression Series 4 (E4)</td><td>Agrobacterium vector with ENTCUP2 promoter</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/b/b9/PORE_E4.gb">link</a></td></tr> | + | <tr><td>V4</td><td><center>-</center></td><td>pORE Expression Series 4 (E4)</td><td>Agrobacterium vector with ENTCUP2 promoter</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/b/b9/PORE_E4.gb">link</a></td></tr> |
| - | <tr><td>V5</td><td>pORE Reporter Series 1 (R1)</td><td>Agrobacterium vector with gusA reporter</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/f/fe/PORE_R1.gb">link</a></td></tr> | + | <tr><td>V5</td><td><center>-</center></td><td>pORE Reporter Series 1 (R1)</td><td>Agrobacterium vector with gusA reporter</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/f/fe/PORE_R1.gb">link</a></td></tr> |
| - | <tr><td>V6</td><td>pORE Reporter Series 3 (R3)</td><td>Agrobacterium vector with smgfp reporter</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/e/e8/PORE_R3.gb">link</a></td></tr> | + | <tr><td>V6</td><td><center>-</center></td><td>pORE Reporter Series 3 (R3)</td><td>Agrobacterium vector with smgfp reporter</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/e/e8/PORE_R3.gb">link</a></td></tr> |
| - | <tr><td>V7</td><td><center>-</center></td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td></td></tr> | + | <tr><td>V7</td><td>BBa_K382000</td><td><center>-</center></td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/3/3b/V7.gb">link</a></td></tr> |
| - | <tr><td>V8</td><td><center>-</center></td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td></td></tr> | + | <tr><td>V8</td><td>BBa_K382001</td><td><center>-</center></td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/0/0f/V8.gb">link</a></td></tr> |
| - | <tr><td>V9</td><td><center>-</center></td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td></td></tr> | + | <tr><td>V9</td><td>BBa_K382002</td><td><center>-</center></td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/d/d2/V9.gb">link</a></td></tr> |
| - | <tr><td>V10</td><td><center>-</center></td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td></td></tr> | + | <tr><td>V10</td><td>BBa_K382003</td><td><center>-</center></td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/3/33/V10.gb">link</a></td></tr> |
| - | <tr><td>V11</td><td><center>-</center></td><td>Agrobacterium vector with gusA reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td></td></tr> | + | <tr><td>V11</td><td>BBa_K382004</td><td><center>-</center></td><td>Agrobacterium vector with gusA reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/5/56/V11.gb">link</a></td></tr> |
| - | <tr><td>V12</td><td><center>-</center></td><td>Agrobacterium vector with smgfp reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td></td></tr> | + | <tr><td>V12</td><td>BBa_K382005</td><td><center>-</center></td><td>Agrobacterium vector with smgfp reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/a/a8/V12.gb">link</a></td></tr> |
</table> | </table> | ||
<br /><br /> | <br /><br /> | ||
| Line 52: | Line 52: | ||
<table cellspacing="0"> | <table cellspacing="0"> | ||
<tr><td><b>Name</b></td><td><b>Description</b></td><td><b>Sequence</b></td></tr> | <tr><td><b>Name</b></td><td><b>Description</b></td><td><b>Sequence</b></td></tr> | ||
| - | <tr><td>VP3</td><td>HindIII-pENTCUP2 forward primer for Expression Series insert</td><td></td></tr> | + | <tr><td>VP3</td><td>HindIII-pENTCUP2 forward primer for Expression Series insert</td><td><a href="https://2010.igem.org/Team:Harvard/VP3">link</a></td></tr> |
| - | <tr><td>VP4</td><td>pENTCUP2-biobrick multiple cloning site-NheI reverse primer for Expression Series insert</td><td></td></tr> | + | <tr><td>VP4</td><td>pENTCUP2-biobrick multiple cloning site-NheI reverse primer for Expression Series insert</td><td><a href="https://2010.igem.org/Team:Harvard/VP4">link</a></td></tr> |
| - | <tr><td>VP5</td><td>HindIII-biobrick multiple cloning site-gusA-forward primer for Reporter Series 1 insert</td><td></td></tr> | + | <tr><td>VP5</td><td>HindIII-biobrick multiple cloning site-gusA-forward primer for Reporter Series 1 insert</td><td><a href="https://2010.igem.org/Team:Harvard/VP5">link</a></td></tr> |
| - | <tr><td>VP6</td><td>gusA-NheI reverse primer for Reporter Series 1 insert</td><td></td></tr> | + | <tr><td>VP6</td><td>gusA-NheI reverse primer for Reporter Series 1 insert</td><td><a href="https://2010.igem.org/Team:Harvard/VP6">link</a></td></tr> |
| - | <tr><td>VP7</td><td>HindIII-biobrick multiple cloning site-smGFP-forward primer for Reporter Series 1 insert</td><td></td></tr> | + | <tr><td>VP7</td><td>HindIII-biobrick multiple cloning site-smGFP-forward primer for Reporter Series 1 insert</td><td><a href="https://2010.igem.org/Team:Harvard/VP7">link</a></td></tr> |
| - | <tr><td>VP8</td><td>smGFP-NheI reverse primer for Reporter Series 1 insert</td><td></td></tr> | + | <tr><td>VP8</td><td>smGFP-NheI reverse primer for Reporter Series 1 insert</td><td><a href="https://2010.igem.org/Team:Harvard/VP8">link</a></td></tr> |
| - | <tr><td>VP9</td><td>Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert</td><td></td></tr> | + | <tr><td>VP9</td><td>Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert</td><td><a href="https://2010.igem.org/Team:Harvard/VP9">link</a></td></tr> |
| - | <tr><td>VP11</td><td>Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert</td><td></td></tr> | + | <tr><td>VP11</td><td>Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert</td><td><a href="https://2010.igem.org/Team:Harvard/VP11">link</a></td></tr> |
| - | <tr><td>VP12</td><td>1 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td></td></tr> | + | <tr><td>VP12</td><td>1 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td><a href="https://2010.igem.org/Team:Harvard/VP12">link</a></td></tr> |
| - | <tr><td>VP13</td><td>2 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td></td></tr> | + | <tr><td>VP13</td><td>2 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td><a href="https://2010.igem.org/Team:Harvard/VP13">link</a></td></tr> |
| - | <tr><td>VP14</td><td>3 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td></td></tr> | + | <tr><td>VP14</td><td>3 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td><a href="https://2010.igem.org/Team:Harvard/VP14">link</a></td></tr> |
| - | <tr><td>VP15</td><td>4 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td></td></tr> | + | <tr><td>VP15</td><td>4 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td><a href="https://2010.igem.org/Team:Harvard/VP15">link</a></td></tr> |
| - | <tr><td>VP16</td><td>5 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td></td></tr> | + | <tr><td>VP16</td><td>5 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td><a href="https://2010.igem.org/Team:Harvard/VP16">link</a></td></tr> |
| - | <tr><td>VP17</td><td>1 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr> | + | <tr><td>VP17</td><td>1 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td><a href="https://2010.igem.org/Team:Harvard/VP17">link</a></td></tr> |
| - | <tr><td>VP18</td><td>2 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr> | + | <tr><td>VP18</td><td>2 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td><a href="https://2010.igem.org/Team:Harvard/VP18">link</a></td></tr> |
| - | <tr><td>VP19</td><td>3 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr> | + | <tr><td>VP19</td><td>3 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td><a href="https://2010.igem.org/Team:Harvard/VP19">link</a></td></tr> |
| - | <tr><td>VP20</td><td>4 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr> | + | <tr><td>VP20</td><td>4 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td><a href="https://2010.igem.org/Team:Harvard/VP20">link</a></td></tr> |
| - | <tr><td>VP21</td><td>5 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr> | + | <tr><td>VP21</td><td>5 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td><a href="https://2010.igem.org/Team:Harvard/VP21">link</a></td></tr> |
| - | <tr><td>VP22</td><td>6 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr> | + | <tr><td>VP22</td><td>6 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td><a href="https://2010.igem.org/Team:Harvard/VP22">link</a></td></tr> |
| - | <tr><td>VP23</td><td>7 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr> | + | <tr><td>VP23</td><td>7 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td><a href="https://2010.igem.org/Team:Harvard/VP23">link</a></td></tr> |
| - | <tr><td>VP24</td><td>8 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr> | + | <tr><td>VP24</td><td>8 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td><a href="https://2010.igem.org/Team:Harvard/VP24">link</a></td></tr> |
| - | <tr><td>VP25</td><td>9 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr> | + | <tr><td>VP25</td><td>9 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td><a href="https://2010.igem.org/Team:Harvard/VP25">link</a></td></tr> |
| - | <tr><td>VP26</td><td>1 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td></td></tr> | + | <tr><td>VP26</td><td>1 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td><a href="https://2010.igem.org/Team:Harvard/VP26">link</a></td></tr> |
| - | <tr><td>VP27</td><td>2 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td></td></tr> | + | <tr><td>VP27</td><td>2 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td><a href="https://2010.igem.org/Team:Harvard/VP27">link</a></td></tr> |
| - | <tr><td>VP28</td><td>3 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td></td></tr> | + | <tr><td>VP28</td><td>3 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td><a href="https://2010.igem.org/Team:Harvard/VP28">link</a></td></tr> |
| - | <tr><td>VP29</td><td>4 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td></td></tr> | + | <tr><td>VP29</td><td>4 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td><a href="https://2010.igem.org/Team:Harvard/VP29">link</a></td></tr> |
| - | <tr><td>VP30</td><td>5 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td></td></tr> | + | <tr><td>VP30</td><td>5 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td><a href="https://2010.igem.org/Team:Harvard/VP30">link</a></td></tr> |
| - | <tr><td> | + | <tr><td>VP31</td><td>1 of 2 for sequencing V7 and V8 biobrick cloning site</td><td><a href="https://2010.igem.org/Team:Harvard/VP31">link</a></td></tr> |
| - | <tr><td>VP34</td><td>2 of 2 for sequencing V7 and V8 biobrick cloning site</td><td></td></tr> | + | <tr><td>VP34</td><td>2 of 2 for sequencing V7 and V8 biobrick cloning site</td><td><a href="https://2010.igem.org/Team:Harvard/VP34">link</a></td></tr> |
Latest revision as of 23:19, 26 October 2010
abstract lab notebook agrobacteria and plant engineering meet the vectors plant transformation parts & primers references
parts and primers
vectors
For more information on the vectors visit our "meet the vectors" page| Name | Biobrick Name | Original Name | Description | Resistance | Resistance (Plants) | Sequence |
| V1 | pORE Open Series 1 (O1) | Agrobacterium vector open series | Kanamycin | pat | link | |
| V2 | pORE Open Series 2 (O2) | Agrobacterium vector open series | Kanamycin | nptII | link | |
| V3 | pORE Expression Series 3 (E3) | Agrobacterium vector with ENTCUP2 promoter | Kanamycin | pat | link | |
| V4 | pORE Expression Series 4 (E4) | Agrobacterium vector with ENTCUP2 promoter | Kanamycin | nptII | link | |
| V5 | pORE Reporter Series 1 (R1) | Agrobacterium vector with gusA reporter | Kanamycin | nptII | link | |
| V6 | pORE Reporter Series 3 (R3) | Agrobacterium vector with smgfp reporter | Kanamycin | nptII | link | |
| V7 | BBa_K382000 | Agrobacterium vector open series with biobrick cloning site | Kanamycin | pat | link | |
| V8 | BBa_K382001 | Agrobacterium vector open series with biobrick cloning site | Kanamycin | nptII | link | |
| V9 | BBa_K382002 | Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site | Kanamycin | pat | link | |
| V10 | BBa_K382003 | Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site | Kanamycin | nptII | link | |
| V11 | BBa_K382004 | Agrobacterium vector with gusA reporter and biobrick cloning site | Kanamycin | nptII | link | |
| V12 | BBa_K382005 | Agrobacterium vector with smgfp reporter and biobrick cloning site | Kanamycin | nptII | link |
primers
| Name | Description | Sequence |
| VP3 | HindIII-pENTCUP2 forward primer for Expression Series insert | link |
| VP4 | pENTCUP2-biobrick multiple cloning site-NheI reverse primer for Expression Series insert | link |
| VP5 | HindIII-biobrick multiple cloning site-gusA-forward primer for Reporter Series 1 insert | link |
| VP6 | gusA-NheI reverse primer for Reporter Series 1 insert | link |
| VP7 | HindIII-biobrick multiple cloning site-smGFP-forward primer for Reporter Series 1 insert | link |
| VP8 | smGFP-NheI reverse primer for Reporter Series 1 insert | link |
| VP9 | Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert | link |
| VP11 | Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert | link |
| VP12 | 1 of 5 for sequencing V12 biobrick cloning site and smgfp reporter | link |
| VP13 | 2 of 5 for sequencing V12 biobrick cloning site and smgfp reporter | link |
| VP14 | 3 of 5 for sequencing V12 biobrick cloning site and smgfp reporter | link |
| VP15 | 4 of 5 for sequencing V12 biobrick cloning site and smgfp reporter | link |
| VP16 | 5 of 5 for sequencing V12 biobrick cloning site and smgfp reporter | link |
| VP17 | 1 of 9 for sequencing V11 biobrick cloning site and gusA reporter | link |
| VP18 | 2 of 9 for sequencing V11 biobrick cloning site and gusA reporter | link |
| VP19 | 3 of 9 for sequencing V11 biobrick cloning site and gusA reporter | link |
| VP20 | 4 of 9 for sequencing V11 biobrick cloning site and gusA reporter | link |
| VP21 | 5 of 9 for sequencing V11 biobrick cloning site and gusA reporter | link |
| VP22 | 6 of 9 for sequencing V11 biobrick cloning site and gusA reporter | link |
| VP23 | 7 of 9 for sequencing V11 biobrick cloning site and gusA reporter | link |
| VP24 | 8 of 9 for sequencing V11 biobrick cloning site and gusA reporter | link |
| VP25 | 9 of 9 for sequencing V11 biobrick cloning site and gusA reporter | link |
| VP26 | 1 of 5 for sequencing V9 and V10 biobrick cloning site and promoter | link |
| VP27 | 2 of 5 for sequencing V9 and V10 biobrick cloning site and promoter | link |
| VP28 | 3 of 5 for sequencing V9 and V10 biobrick cloning site and promoter | link |
| VP29 | 4 of 5 for sequencing V9 and V10 biobrick cloning site and promoter | link |
| VP30 | 5 of 5 for sequencing V9 and V10 biobrick cloning site and promoter | link |
| VP31 | 1 of 2 for sequencing V7 and V8 biobrick cloning site | link |
| VP34 | 2 of 2 for sequencing V7 and V8 biobrick cloning site | link |
