Team:LMU-Munich/Jump-or-die/Schedule/SequenzPCR3

From 2010.igem.org

(Difference between revisions)

Latest revision as of 17:23, 24 October 2010


	Cut'N'Survive System Functional Principle Sequences Schedule Jump-or-Die System Functional Principle Sequences Schedule ProSearch System Schedule

	Functional Principle Sequences Schedule picture gallery

Sequence PCR3

with forward primer (primer 3)

NNNNN

with reverse primer (primer 6)

GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN

verified by sequencing of BBa_K368016 (PCR 3 in pSB1C3)

with forward primer

NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTNNAGAGANNGATGAATTG

NTNAGGNNGNNNTNTTCTTTNTACAAGAAGGCTGGTTCTGAAAACTTGTACTTCCA

ATTCCACAAGTCTGGTGCTTGGAANNTGACANTTTCTTNGGTTAANANAGGGATCGA

TAAGCTTGATTATAAANAACANCTTCNGGCTTGGCGGTGGGAAAGAGAAATTGATG

ANAGAANTCGCCCACTTTCTGATGANGAATTAGATGCTATGTTCCCANAANGATATA

AGGTACTTCCTCCTNCAGCTGGTTATGTTCNTATTCGAACTCCANCTCATATGGAT

CGATTCCNGCANNAGCANGTGCTGGTGCTGAAGTTGCTGTTGCNAGNCNNGNGATTN

ACCACCNAAATTANGTCATTTAGGANCNTACTANCACCCCCCTCCTGCNNTCCNGC

AAANNN

with reverse primer

NNNNNNNNNNNNNNNNNNNGACCNNNCGCAGCGAGTCAGTGAGNNAGGAAGCCTGCA

TAACGCGAAGTAATCTTTTCGGTTTNAANGTGNNGGCAGGGTGGTGACACCTTGCCC

TTTTTTGCCGGATTGNAGCGGNCGCTACTAGTAGGATCCTTTATGACCTAATTTAGA

TGGTAAATCACAATATCTAGCAACAGCAACTTCAGCNCCAGCACCTGCTACTGCCGG

AATCGATCCATATGAGCTGGAGTTCGAATAGGAACATAACCAGCTGGAGGAGGAAGT

ACCTTATATCCTTCTGGGAACATAGCATCTAATTCCTCATCAGAAAGTGGGCGATT

TCTCTCATCAATTTCTCTTTCCCACCGCCAAGCCTGAAGCTGTTCTTTATAATCAAG

CTTATCGATCCCTCTCTTAACCAAAGAAACTGGCAACTTCCAAGCACCAGACTTGTG

GAATTGGAAGTACAAGTTTTCAGAACCAGCCTTCTTGTACAAAGAAGTAATAGATTTG

TACAATTCATCCATCTCTAGAAGCGGCCGCGAATTCCANAAATCATCCTTAGCGAAAG

CTAAGGATTTTTTTTATCTGAAATTCTGCCTCGTGATACGCCTATTTTTATAGGTTAA

TGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTCGAGTTGATCGGGCACGT

AAGAGGTTCCAACTTTCACCATAATGAAATAAGATCACTACCGGGCGTATTTTTTGA

GTTATCGAGATTTTCANGAGCTAANGANCTAAATGGAGAAAAAATCACTGGATATANC

ACCGTTGATATATCCCAATGGCATCGTAAAGAACANTTTGAGGCATTTCAGTCAGTTG

CTCNATGTACCTATAACCAGACCGTCANCTGGATNTACGGCCTTTTTANAGACCGNAA

GAAAATAAGCACAAGTTTATCCGGCTTATCACATTCCTGCNGCNNGATGAATGCTCAT

CCGGAATTTCNATGGCNATGAAAGACGGNGANCTGGTGATATGGGNNANNGNTNNNCC

TNGNNNACCNTTTCCNNNNNNAACTGNANN

@@ Line 7: / Line 7: @@
 === with reverse primer (primer 6)===
-GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNN
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
@@ Line 13: / Line 14: @@
 === with forward primer===
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTNNAGAGANNGATGAATTGNTNAGGNNGNNNTNTTCTTTNTACAAGAAGGCTGGTTCTGAAAACTTGTACTTCCAATTCCACAAGTCTGGTGCTTGGAANNTGACANTTTCTTNGGTTAANANAGGGATCGATAAGCTTGATTATAAANAACANCTTCNGGCTTGGCGGTGGGAAAGAGAAATTGATGANAGAANTCGCCCACTTTCTGATGANGAATTAGATGCTATGTTCCCANAANGATATAAGGTACTTCCTCCTNCAGCTGGTTATGTTCNTATTCGAACTCCANCTCATATGGATCGATTCCNGCANNAGCANGTGCTGGTGCTGAAGTTGCTGTTGCNAGNCNNGNGATTNACCACCNAAATTANGTCATTTAGGANCNTACTANCACCCCCCTCCTGCNNTCCNGCAAANNN
+NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTNNAGAGANNGATGAATTG
+NTNAGGNNGNNNTNTTCTTTNTACAAGAAGGCTGGTTCTGAAAACTTGTACTTCCA
+ATTCCACAAGTCTGGTGCTTGGAANNTGACANTTTCTTNGGTTAANANAGGGATCGA
+TAAGCTTGATTATAAANAACANCTTCNGGCTTGGCGGTGGGAAAGAGAAATTGATG
+ANAGAANTCGCCCACTTTCTGATGANGAATTAGATGCTATGTTCCCANAANGATATA
+AGGTACTTCCTCCTNCAGCTGGTTATGTTCNTATTCGAACTCCANCTCATATGGAT
+CGATTCCNGCANNAGCANGTGCTGGTGCTGAAGTTGCTGTTGCNAGNCNNGNGATTN
+ACCACCNAAATTANGTCATTTAGGANCNTACTANCACCCCCCTCCTGCNNTCCNGC
+AAANNN
 === with reverse primer===
-NNNNNNNNNNNNNNNNNNNGACCNNNCGCAGCGAGTCAGTGAGNNAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGTTTNAANGTGNNGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGATTGNAGCGGNCGCTACTAGTAGGATCCTTTATGACCTAATTTAGATGGTAAATCACAATATCTAGCAACAGCAACTTCAGCNCCAGCACCTGCTACTGCCGGAATCGATCCATATGAGCTGGAGTTCGAATAGGAACATAACCAGCTGGAGGAGGAAGTACCTTATATCCTTCTGGGAACATAGCATCTAATTCCTCATCAGAAAGTGGGCGATTTCTCTCATCAATTTCTCTTTCCCACCGCCAAGCCTGAAGCTGTTCTTTATAATCAAGCTTATCGATCCCTCTCTTAACCAAAGAAACTGGCAACTTCCAAGCACCAGACTTGTGGAATTGGAAGTACAAGTTTTCAGAACCAGCCTTCTTGTACAAAGAAGTAATAGATTTGTACAATTCATCCATCTCTAGAAGCGGCCGCGAATTCCANAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATTCTGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTCGAGTTGATCGGGCACGTAAGAGGTTCCAACTTTCACCATAATGAAATAAGATCACTACCGGGCGTATTTTTTGAGTTATCGAGATTTTCANGAGCTAANGANCTAAATGGAGAAAAAATCACTGGATATANCACCGTTGATATATCCCAATGGCATCGTAAAGAACANTTTGAGGCATTTCAGTCAGTTGCTCNATGTACCTATAACCAGACCGTCANCTGGATNTACGGCCTTTTTANAGACCGNAAGAAAATAAGCACAAGTTTATCCGGCTTATCACATTCCTGCNGCNNGATGAATGCTCATCCGGAATTTCNATGGCNATGAAAGACGGNGANCTGGTGATATGGGNNANNGNTNNNCCTNGNNNACCNTTTCCNNNNNNAACTGNANN
+NNNNNNNNNNNNNNNNNNNGACCNNNCGCAGCGAGTCAGTGAGNNAGGAAGCCTGCA
+TAACGCGAAGTAATCTTTTCGGTTTNAANGTGNNGGCAGGGTGGTGACACCTTGCCC
+TTTTTTGCCGGATTGNAGCGGNCGCTACTAGTAGGATCCTTTATGACCTAATTTAGA
+TGGTAAATCACAATATCTAGCAACAGCAACTTCAGCNCCAGCACCTGCTACTGCCGG
+AATCGATCCATATGAGCTGGAGTTCGAATAGGAACATAACCAGCTGGAGGAGGAAGT
+ACCTTATATCCTTCTGGGAACATAGCATCTAATTCCTCATCAGAAAGTGGGCGATT
+TCTCTCATCAATTTCTCTTTCCCACCGCCAAGCCTGAAGCTGTTCTTTATAATCAAG
+CTTATCGATCCCTCTCTTAACCAAAGAAACTGGCAACTTCCAAGCACCAGACTTGTG
+GAATTGGAAGTACAAGTTTTCAGAACCAGCCTTCTTGTACAAAGAAGTAATAGATTTG
+TACAATTCATCCATCTCTAGAAGCGGCCGCGAATTCCANAAATCATCCTTAGCGAAAG
+CTAAGGATTTTTTTTATCTGAAATTCTGCCTCGTGATACGCCTATTTTTATAGGTTAA
+TGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTCGAGTTGATCGGGCACGT
+AAGAGGTTCCAACTTTCACCATAATGAAATAAGATCACTACCGGGCGTATTTTTTGA
+GTTATCGAGATTTTCANGAGCTAANGANCTAAATGGAGAAAAAATCACTGGATATANC
+ACCGTTGATATATCCCAATGGCATCGTAAAGAACANTTTGAGGCATTTCAGTCAGTTG
+CTCNATGTACCTATAACCAGACCGTCANCTGGATNTACGGCCTTTTTANAGACCGNAA
+GAAAATAAGCACAAGTTTATCCGGCTTATCACATTCCTGCNGCNNGATGAATGCTCAT
+CCGGAATTTCNATGGCNATGAAAGACGGNGANCTGGTGATATGGGNNANNGNTNNNCC
+TNGNNNACCNTTTCCNNNNNNAACTGNANN
 {{:Team:LMU-Munich/Templates/Page Footer}}