From 2010.igem.org
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| - | {{Stockholm/Top2}} | + | {{Stockholm/modelling}} |
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| - | ==<s>Production Phase</s>== | + | <html><center><img src="https://static.igem.org/mediawiki/2010/a/a0/SU_modelling_Icon.gif"></center></html> |
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| - | <s>
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| - | ;Suggested Articles
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| - | http://www.ncbi.nlm.nih.gov/pubmed/17536514
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| - | http://www.ncbi.nlm.nih.gov/pubmed/17571257
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| - | http://www.ncbi.nlm.nih.gov/pubmed/19892171
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| - | http://www.ncbi.nlm.nih.gov/pubmed/20201800
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| - | '''Hassan:''' For production phase, there are several cases that we need to take care of. One of interesting subjects is "inclusion bodies". As Johan and Nina said, it is better to study about over expression.</s>
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| - | ==<s>Secretion</s>==
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| - | <s>
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| - | http://www.ncbi.nlm.nih.gov/pubmed/15763404
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| - | http://www.ncbi.nlm.nih.gov/pubmed/19597765
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| - | </s>
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| - | ==PPI==
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| - | ''"It has become dangerously acceptable to conclude that proteins interact directly provided that (i) the interaction is biologically plausible; (ii) the proteins are co-expressed; and (iii) an interaction is demonstrated by glutathione-S-transferase (GST)-pulldowns and/or co-immunoprecipitations (co-IPs)."''
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| - | ''"The most obvious caveat with co-IP data is that a positive result does not imply a direct interaction between two proteins – binding could be mediated by other partners."''
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| - | "Protein interactions: is seeing believing? J. P. Mackay et al. 2007"
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Latest revision as of 12:24, 25 October 2010
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