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Team:Groningen/18 October 2010
From 2010.igem.org
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'''Modellers:''' Find constants and parameters for gene expression model, finish modelling and put everything on the wiki ''Laura, Djoke'' | '''Modellers:''' Find constants and parameters for gene expression model, finish modelling and put everything on the wiki ''Laura, Djoke'' | ||
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| + | Write wiki text for killswitch and gene expression model. Create all figures, tables and equations in wiki format for gene expression model and killswitch model. ''Laura'' | ||
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| + | <br> | ||
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| + | '''Peter en David''' | ||
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| + | Expression experiment contruct E&H chaplin and C chaplin. Growing in batches of 0.5 L liquid TY medium at 37 degrees. Inoculation with overnight culture with a starting OD of 0.1. Inducing at an OD of 0.5 with 1.5% subtilin overnight. Harvesting the overnight culture and washing pellet before disrupting it. | ||
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| + | THT measurement | ||
| + | [[Image:C CN EH EHN gr.jpg]] | ||
| + | <br> | ||
| + | Mass spec malditov results | ||
| + | <br> | ||
| + | [[Image:massspec malditov disruption]] | ||
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| + | '''David''' | ||
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| + | measurements in Bremen on surface activity of chaplin coated objects | ||
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| + | We wanted to know if there was an effect of our chaplin proteins on surface drag in the water. To test this we wanted to measure the water resistance of a spindel shaped object in the water at different speeds. First do the measurements with a clean spindel and secondly after coating the spindel with purified chaplins from streptomyces. The spindle that was used for the mesurements, this shapes is designed minimize the effect of shape of the objects drag. Thereby maximizing the effect of surface resistance on the drag coeffient. | ||
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| + | [[Image:pinnacleunitvandennis.jpg]] | ||
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| + | <br> | ||
Latest revision as of 03:54, 28 October 2010
Modellers: Find constants and parameters for gene expression model, finish modelling and put everything on the wiki Laura, Djoke
Write wiki text for killswitch and gene expression model. Create all figures, tables and equations in wiki format for gene expression model and killswitch model. Laura
Peter en David
Expression experiment contruct E&H chaplin and C chaplin. Growing in batches of 0.5 L liquid TY medium at 37 degrees. Inoculation with overnight culture with a starting OD of 0.1. Inducing at an OD of 0.5 with 1.5% subtilin overnight. Harvesting the overnight culture and washing pellet before disrupting it.
THT measurement
File:C CN EH EHN gr.jpg
Mass spec malditov results
File:Massspec malditov disruption
David
measurements in Bremen on surface activity of chaplin coated objects
We wanted to know if there was an effect of our chaplin proteins on surface drag in the water. To test this we wanted to measure the water resistance of a spindel shaped object in the water at different speeds. First do the measurements with a clean spindel and secondly after coating the spindel with purified chaplins from streptomyces. The spindle that was used for the mesurements, this shapes is designed minimize the effect of shape of the objects drag. Thereby maximizing the effect of surface resistance on the drag coeffient.
