Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/21

From 2010.igem.org

(Difference between revisions)
(→Experiment:Transformation)
 
(11 intermediate revisions not shown)
Line 1: Line 1:
{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
-
 
+
__NOTOC__
==2010/9/21 Tuesday (watachin)==
==2010/9/21 Tuesday (watachin)==
===Experiment:Miniprep (extraction of plasmid kit)===
===Experiment:Miniprep (extraction of plasmid kit)===
-
'''Member'''<br />
+
:'''Member'''<br />
-
easily and NEX
+
:easily and NEX
-
 
+
-
'''Materials and Procedure'''
+
-
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Miniprep_.28extraction_of_plasmid_kit.29">the protocol(Miniprep)</a></html>
+
:'''Materials and Procedure'''
 +
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Miniprep_.28extraction_of_plasmid_kit.29">protocol10</a></html>
===Experiment:PCR===
===Experiment:PCR===
-
'''Member'''<br />
+
:'''Member'''<br />
-
watachin
+
:watachin
-
'''Materials and Procedure'''
+
:'''Materials and Procedure'''
-
 
+
-
PCR of pSB1C3
+
-
 
+
-
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">the protocol(Direct PCR)</a></html>
+
 +
:PCR of pSB1C3
 +
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">protocol3</a></html>
===Experiment:Electrophoresis===
===Experiment:Electrophoresis===
-
'''Member'''<br />
+
:'''Member'''<br />
-
easily
+
:easily
-
'''Materials and Procedure'''
+
:'''Materials and Procedure'''
-
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">the protocol(Electrophoresis)</a></html>
+
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">protocol4</a></html>
-
'''Result'''
+
:'''Result'''
[[Image:2010-09-21-01.jpg]]
[[Image:2010-09-21-01.jpg]]
[[Image:2010-09-21-02.jpg]]
[[Image:2010-09-21-02.jpg]]
-
===Experiment:DNA purification from agarose gel with QIAGEN===
+
===Experiment:DNA purification from agarose gel===
-
'''Member'''<br />
+
:'''Member'''<br />
-
watachin
+
:watachin
-
'''Materials and Procedure'''
+
:'''Materials and Procedure'''
-
 
+
-
 
+
-
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">the protocol(DNA purification from agarose gel with QIAGEN)</a></html>
+
 +
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">protocol5</a></html>
===Experiment:Digestion===
===Experiment:Digestion===
-
'''Member'''<br />
+
:'''Member'''<br />
-
NEX and watachin
+
:NEX and watachin
-
'''Materials and Procedure'''
+
:'''Materials and Procedure'''
-
 
+
-
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Digestion">the protocol(Digestion)</a></html>
+
-
 
+
-
===Experiment:Digestion===
+
-
'''Member'''<br />
+
-
NEX and watachin
+
-
 
+
-
'''Materials and Procedure'''
+
-
 
+
-
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Digestion">the protocol(Digestion)</a></html>
+
 +
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Digestion">protocol7</a></html>
===Experiment:Electrophoresis===
===Experiment:Electrophoresis===
-
'''Member'''<br />
+
:'''Member'''<br />
-
NEX and watachin
+
:NEX and watachin
-
'''Materials and Procedure'''
+
:'''Materials and Procedure'''
 +
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">protocol5</a></html>
-
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">the protocol(Electrophoresis)</a></html>
+
:'''Result'''
-
 
+
-
 
+
-
'''Result'''
+
[[Image:2010-09-21-03.jpg]]
[[Image:2010-09-21-03.jpg]]
 +
===Experiment:DNA Extraction from agarose gel===
 +
:'''Member'''<br />
 +
:NEX and watachin
-
===Experiment:DNA purification from agarose gel with QIAGEN===
+
:'''Materials and Procedure'''
-
'''Member'''<br />
+
-
NEX and watachin
+
-
 
+
-
'''Materials and Procedure'''
+
-
 
+
-
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">the protocol(DNA purification from agarose gel with QIAGEN)</a></html>
+
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">protocol5</a></html>
===Experiment:Electrophoresis===
===Experiment:Electrophoresis===
-
'''Member'''<br />
+
:'''Member'''<br />
-
watachin
+
:watachin
-
'''Materials and Procedure'''
+
:'''Materials and Procedure'''
 +
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">protocol4</a></html>
-
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">the protocol(Electrophoresis)</a></html>
+
:'''Result'''
-
 
+
-
 
+
-
'''Result'''
+
[[Image:2010-09-21-04.jpg]]
[[Image:2010-09-21-04.jpg]]
[[Image:2010-09-21-05.jpg]]
[[Image:2010-09-21-05.jpg]]
 +
===Experiment:DNA Extraction from agarose gel===
 +
:'''Member'''<br />
 +
:watachin
-
===Experiment:DNA purification from agarose gel with QIAGEN===
+
:'''Materials and Procedure'''
-
'''Member'''<br />
+
-
watachin
+
-
'''Materials and Procedure'''
+
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">protocol5</a></html>
-
 
+
-
 
+
-
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">the protocol(DNA purification from agarose gel with QIAGEN)</a></html>
+
===Experiment:Transformation===
===Experiment:Transformation===
-
'''Member'''<br />
+
:'''Member'''<br />
-
watachin
+
:watachin
-
 
+
-
'''Materials and Procedure'''
+
 +
:'''Materials and Procedure'''
-
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Transformation">the protocol(Transformation)</a></html>
+
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Transformation">protocol9</a></html>

Latest revision as of 14:02, 27 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
September 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October 2010
SUNMONTUEWEDTHUFRISAT
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

2010/9/21 Tuesday (watachin)

Experiment:Miniprep (extraction of plasmid kit)

Member
easily and NEX
Materials and Procedure
follow protocol10

Experiment:PCR

Member
watachin
Materials and Procedure
PCR of pSB1C3
follow protocol3

Experiment:Electrophoresis

Member
easily
Materials and Procedure
follow protocol4
Result

2010-09-21-01.jpg 2010-09-21-02.jpg

Experiment:DNA purification from agarose gel

Member
watachin
Materials and Procedure
follow protocol5

Experiment:Digestion

Member
NEX and watachin
Materials and Procedure
follow protocol7

Experiment:Electrophoresis

Member
NEX and watachin
Materials and Procedure
follow protocol5
Result

2010-09-21-03.jpg

Experiment:DNA Extraction from agarose gel

Member
NEX and watachin
Materials and Procedure
follow protocol5

Experiment:Electrophoresis

Member
watachin
Materials and Procedure
follow protocol4
Result

2010-09-21-04.jpg 2010-09-21-05.jpg

Experiment:DNA Extraction from agarose gel

Member
watachin
Materials and Procedure
follow protocol5

Experiment:Transformation

Member
watachin
Materials and Procedure
follow protocol9
Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Fiber/2010/09/21"