BIOTEC Dresden/Notepad/19 October 2010

From 2010.igem.org

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50 colonies were picked from the ligation plates of the fusion protein for colony PCR. Colonies were picked for the overnight cultures.
50 colonies were picked from the ligation plates of the fusion protein for colony PCR. Colonies were picked for the overnight cultures.
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'''AHL Sensor'''
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<hr>
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<p>Two plates were measured: 1751 in Ampicillin and 1751 in Chloramphenicol</p>
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<p>Miniprep of overnight cultures of 17+51</p>
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[[Category:BIOTEC Dresden/Notepad/October|October]]
{{Biotec_Dresden/month}}
{{Biotec_Dresden/month}}
{{Biotec_Dresden/Bottom}}
{{Biotec_Dresden/Bottom}}

Latest revision as of 23:15, 27 October 2010

Parts Assembly

All the parts need to be ligated into the plasmid backbone containing chloramphenicol as per iGeM requirement. The following parts were restriction digested with EcoRI and PstI for 1 hour 15 min at 37 degrees.

52b, 52c, 53a, 53b, 53c, 54a, 54b, 54c, 55a, 55b, 55c, backbone, 6e-4a

The digested parts were purified by gel purification and nanodropped.

Fusion Protein

50 colonies were picked from the ligation plates of the fusion protein for colony PCR. Colonies were picked for the overnight cultures.

AHL Sensor

Two plates were measured: 1751 in Ampicillin and 1751 in Chloramphenicol

Miniprep of overnight cultures of 17+51


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