From 2010.igem.org
(Difference between revisions)
|
|
| (2 intermediate revisions not shown) |
| Line 40: |
Line 40: |
| | * <partinfo>pSB1AK3</partinfo> - <partinfo>BBa_K300009</partinfo> (negative control) | | * <partinfo>pSB1AK3</partinfo> - <partinfo>BBa_K300009</partinfo> (negative control) |
| | | | |
| - | The light-blue wells refer to the same cultures grown over night; as the results were the same of the cultures grown for about six hours, from now on we have followed only the steps shown in [[Team:UNIPV-Pavia/Parts/Characterization#Microplate reader experiments for self-inducible promoters - Protocol #3|Protocol 3]]. | + | The light-blue wells refer to the same cultures grown over night; as the results were the same of the cultures grown for about six hours, from now on we have followed only the steps shown in [[Team:UNIPV-Pavia/Parts/Characterization#Microplate reader experiments for self-inducible promoters - Protocol #3|Protocol 3]]. All supernatants were diluted 1:100. |
| | | | |
| | ==RESULTS== | | ==RESULTS== |
| Line 51: |
Line 51: |
| | || Part || 3OC6-HSL concentration [uM] | | || Part || 3OC6-HSL concentration [uM] |
| | |- | | |- |
| - | || || | + | || <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300030</partinfo> || ~0.7 |
| | |- | | |- |
| - | || || | + | || <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300028</partinfo> || ~0.04 |
| | |- | | |- |
| - | || || | + | || <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300025</partinfo> || ~0.09 |
| | |} | | |} |
| | | | |
| - | Because supernatants were not properly diluted it was not possible to measure some of the 3OC6-HSL concentrations; a more accurate evaluation of 3OC6-HSL was performed in the next test. | + | Because supernatants were not properly diluted it was not possible to measure some of the 3OC6-HSL concentrations; a more accurate evaluation of 3OC6-HSL was performed in the [[Team:UNIPV-Pavia/Material_Methods/Measurements/Tecan/test10settembre_bis|next test]]. |
| | | | |
| | <!-- table previous next test --> | | <!-- table previous next test --> |
Latest revision as of 09:55, 23 October 2010
|
|
|
|
|
SEPTEMBER, 8TH
PLATE
EXPERIMENT DESCRIPTION
Motivation
The experiment was performed to measure the 3OC6-HSL concentration due to the synthesis of homoserine-lactone by 3OC6-HSL generators in saturation phase of the cultures growth.
Methods
According to Protocol 3 the experiment was performed in LB on the following signalling parts:
- <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300030</partinfo> (wiki name: I14)
- <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300028</partinfo> (wiki name: I15)
- <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300029</partinfo> (wiki name: I16)
- <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300025</partinfo> (wiki name: I17)
- <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300026</partinfo> (wiki name: I18)
- <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300027</partinfo> (wiki name: I19)
- <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> (wiki name: I14-4C5)
- <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> (wiki name: I15-4C5)
- <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> (wiki name: I16-4C5)
- <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> (wiki name: I17-4C5)
- <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> (wiki name: I18-4C5)
- <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> (wiki name: I19-4C5)
- <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0031</partinfo> (negative control)
- <partinfo>pSB1AK3</partinfo> - <partinfo>BBa_K300009</partinfo> (negative control)
The light-blue wells refer to the same cultures grown over night; as the results were the same of the cultures grown for about six hours, from now on we have followed only the steps shown in Protocol 3. All supernatants were diluted 1:100.
RESULTS
It was possible to evaluate some of the 3OC6-HSL concentrations:
| Part | 3OC6-HSL concentration [uM]
|
| <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300030</partinfo> | ~0.7
|
| <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300028</partinfo> | ~0.04
|
| <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300025</partinfo> | ~0.09
|
Because supernatants were not properly diluted it was not possible to measure some of the 3OC6-HSL concentrations; a more accurate evaluation of 3OC6-HSL was performed in the next test.
|
|
|
"
