Team:Northwestern/References

From 2010.igem.org

(Difference between revisions)
 
(One intermediate revision not shown)
Line 110: Line 110:
<!--- The Mission, Experiments --->
<!--- The Mission, Experiments --->
-
{|- style="font-size: 87%; background: #AFC7C7; color: #000000" cellpadding="1" cellspacing="1" bordercolor="#000000" width="75%" align="center"
+
{|- style="font-size: 87%; background: #EFCDF8; color: #FFFFFF" cellpadding="1" cellspacing="1" bordercolor="#000000" width="75%" align="center"
-
!align="center"|[[Team:Northwestern|<font color="#FFFFFF">'''Home'''</font>]]
+
!align="center"|[[Team:Northwestern|<font color="#2B3856">'''Home'''</font>]]
-
!align="center"|[[Team:Northwestern/Brainstorm|<font color="#FFFFFF">'''Brainstorm'''</font>]]
+
!align="center"|[[Team:Northwestern/Brainstorm|<font color="#000000">'''Brainstorm'''</font>]]
-
!align="center"|[[Team:Northwestern/Team|<font color="#FFFFFF">'''Team'''</font>]]
+
!align="center"|[[Team:Northwestern/Team|<font color="#000000">'''Team'''</font>]]
-
!align="center"|[[Team:Northwestern/Acknowledgements|<font color="#FFFFFF">'''Acknowledgements'''</font>]]
+
!align="center"|[[Team:Northwestern/Acknowledgements|<font color="#000000">'''Acknowledgements'''</font>]]
-
!align="center"|[[Team:Northwestern/Project|<font color="#FFFFFF">'''Project'''</font>]]
+
!align="center"|[[Team:Northwestern/Project|<font color="#000000">'''Project'''</font>]]
-
!align="center"|[[Team:Northwestern/SideProject|<font color="#FFFFFF">'''Human Practices'''</font>]]
+
!align="center"|[[Team:Northwestern/SideProject|<font color="#000000">'''Human Practices'''</font>]]
-
!align="center"|[[Team:Northwestern/Parts|<font color="#FFFFFF">'''Parts'''</font>]]
+
!align="center"|[[Team:Northwestern/Parts|<font color="#000000">'''Parts'''</font>]]
-
!align="center"|[[Team:Northwestern/Notebook|<font color="#FFFFFF">'''Notebook'''</font>]]
+
!align="center"|[[Team:Northwestern/Notebook|<font color="#000000">'''Notebook'''</font>]]
-
!align="center"|[[Team:Northwestern/Calendar|<font color="#FFFFFF">'''Calendar'''</font>]]
+
!align="center"|[[Team:Northwestern/Calendar|<font color="#000000">'''Calendar'''</font>]]
-
!align="center"|[[Team:Northwestern/Protocol|<font color="#FFFFFF">'''Protocol'''</font>]]
+
!align="center"|[[Team:Northwestern/Protocol|<font color="#000000">'''Protocol'''</font>]]
-
!align="center"|[[Team:Northwestern/Safety|<font color="#FFFFFF">'''Safety'''</font>]]
+
!align="center"|[[Team:Northwestern/Safety|<font color="#000000">'''Safety'''</font>]]
-
!align="center"|[[Team:Northwestern/Links|<font color="#FFFFFF">'''Links'''</font>]]
+
!align="center"|[[Team:Northwestern/Links|<font color="#000000">'''Links'''</font>]]
-
!align="center"|[[Team:Northwestern/References|<font color="#2B3856">'''References'''</font>]]
+
!align="center"|[[Team:Northwestern/References|<font color="#000000">'''References'''</font>]]
-
!align="center"|[[Team:Northwestern/Media|<font color="#FFFFFF">'''Media'''</font>]]
+
!align="center"|[[Team:Northwestern/Media|<font color="#000000">'''Media'''</font>]]
-
!align="center"|[[Team:Northwestern/Contact|<font color="#FFFFFF">'''Contact'''</font>]]
+
!align="center"|[[Team:Northwestern/Contact|<font color="#000000">'''Contact'''</font>]]
|}
|}
{| align="center" border="0" width="75%"
{| align="center" border="0" width="75%"
|
|
 +
=='''References'''==
=== Lac Operon Control ===
=== Lac Operon Control ===

Latest revision as of 01:18, 27 October 2010


Tech Institute
Home Brainstorm Team Acknowledgements Project Human Practices Parts Notebook Calendar Protocol Safety Links References Media Contact

References

Lac Operon Control

Lac Operon induction and IPTG: http://openwetware.org/wiki/IPTG

Chitin

Adding Glucosamine in medium: http://ec.asm.org/cgi/reprint/2/5/886

Chitin synthesis Pathway (image): [http://www.mekarn.org/msc2005-07/thesis07/latslrimage002.jpg]

Chitin Precursor (UDP-N-Acetyl-Glucosamine) is used for Endotoxin (Lipid A) production: http://www.jbc.org/content/268/26/19858.long

Uses for Chitin: http://www.gmp-chitosan.com/en/products-services/chitin.html

Chitin Synthesis

Chitin Synthase Gene (CHS3): http://www.ncbi.nlm.nih.gov/gene/852311

CHS3 polymerizes: http://www.ebi.ac.uk/interpro/IEntry?ac=IPR004835, http://www.uniprot.org/uniprot/P29465

Chitin Staining (Calcofluor)

Effect on e. coli: http://www.sigmaaldrich.com/etc/medialib/docs/Fluka/Datasheet/18909dat.pdf

Staining of Biofilm Polysaccharides- http://tinyurl.com/36pn4za

Inhibition of Chitin Synthase - http://www.ncbi.nlm.nih.gov/pmc/articles/instance/40653/

Data sheet: http://tinyurl.com/283wax2

Excitation/Emission: http://www.olympusmicro.com/primer/techniques/fluorescence/fluorotable2.html

Apoptosis

partsregistry: http://partsregistry.org/Cell_death

Live/Dead Staining aka Viability/Cytotoxicity Assay

DMAO(Live), EtD-III(Dead)

  • http://www.promokine.info/products/cell-analysis/cell-staining-reagents-related-products/
  • http://www.biotium.com/product/product_info/Protocol/30027.pdf

Keio Collection - KnockOuts

Keio Website: http://ecoli.naist.jp/gb6/Resources/deletion/deletion.html

Ordering: http://www.shigen.nig.ac.jp/ecoli/strain/top/top.jsp

Method: http://www.pnas.org/content/97/12/6640.full.pdf+html

Journal Paper: http://www.nature.com/msb/journal/v2/n1/full/msb4100050.html

Primer Extensions: http://www.biomedsearch.com/attachments/00/16/73/85/16738554/msb4100050-s4.xls

ChiA (Chitinase) Knockout

Summary of e. coli knockouts: http://openwetware.org/wiki/Escherichia_coli/Knockouts

Chitinase gene (chiA): http://www.ncbi.nlm.nih.gov/gene/947837

chiA-knockout strain e coli: http://ecoli.naist.jp/GB6/info.jsp?id=JW3300

ydgg/tqsA (AI2-Transporter) Knockout

Knockout biofilm formation: http://jb.asm.org/cgi/content/short/188/2/587, http://ecoli.aist-nara.ac.jp/GB5/info.jsp?id=JW1593

more info: http://www.open-access-biology.com/biofilms/biofilmsch7.pdf

BIF (Biological Imaging Facility)

List of Equipment: http://www.northwestern.edu/bioimaging/equip.html
Methods: http://www.northwestern.edu/bioimaging/methods.html


Retrieved from "http://2010.igem.org/Team:Northwestern/References"