Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test2settembre

From 2010.igem.org

(Difference between revisions)
m
m (→RESULTS)
 
(2 intermediate revisions not shown)
Line 43: Line 43:
* <partinfo>BBa_B0031</partinfo> (negative control)
* <partinfo>BBa_B0031</partinfo> (negative control)
-
==RESULTS (?)==
+
==RESULTS==
 +
 
 +
[[Image:UNIPV_Pavia_HCpromotersM9.png| 620px | center ]]
 +
 
 +
In high copy number plasmids it is possible to identify 3 groups: strong promoters (<partinfo>Ba_J23100</partinfo> and <partinfo>BBa_J23101</partinfo>), medium strength promoters (in order <partinfo>BBa_J23110</partinfo> and <partinfo>BBa_J23118</partinfo>) and a group of weak promoters.
 +
 
 +
{| border='1' align='center'
 +
|| Promoter || R.P.U. (average) || std
 +
|-
 +
|| <partinfo>BBa_J23100</partinfo> || 0.946 || 0.182
 +
|-
 +
|| <partinfo>BBa_J23101</partinfo> || 1 || -
 +
|-
 +
|| <partinfo>BBa_J23105</partinfo> || 0.196 || 0.038
 +
|-
 +
|| <partinfo>BBa_J23106</partinfo> || 0.326 || 0.015
 +
|-
 +
|| <partinfo>BBa_J23110</partinfo> || 0.678 || 0.096
 +
|-
 +
|| <partinfo>BBa_J23114</partinfo> || 0.091 || 0.022
 +
|-
 +
|| <partinfo>BBa_J23116</partinfo> || 0.101 || 0.004
 +
|-
 +
|| <partinfo>BBa_J23118</partinfo> || 0.655 || 0.035
 +
|}
 +
 
 +
[[Image:UNIPV_Pavia_LCpromotersM9.png| 620px | center ]]
 +
 
 +
In low copy number plasmids there are the same 3 groups but <partinfo>BBa_J23118</partinfo> resulted stronger than <partinfo>BBa_J23110</partinfo>.
 +
 
 +
{| border='1' align='center'
 +
|| Promoter || R.P.U. (average) || std
 +
|-
 +
|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23100</partinfo> || 0.838 || 0.101
 +
|-
 +
|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23101</partinfo> || 1 || -
 +
|-
 +
|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23105</partinfo> || 0.149 || 0.045
 +
|-
 +
|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23106</partinfo> || 0.286 || 0.033
 +
|-
 +
|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23110</partinfo> || 0.466 || 0.101
 +
|-
 +
|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23114</partinfo> || 0.053 || 0.048
 +
|-
 +
|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23116</partinfo> || 0.110 || 0.007
 +
|-
 +
|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23118</partinfo> || 0.714 || 0.154
 +
|}
<!-- table previous next test -->
<!-- table previous next test -->

Latest revision as of 14:10, 27 October 2010
Home
The Project
Materials & Methods
Parts
The Team
Notebook
Biological Safety
Pavia
Gallery
Sponsors

SEPTEMBER, 2ND


PLATE


UNIPV Pavia piastra2settembre.png


EXPERIMENT DESCRIPTION

Motivation

This experiment was performed in M9 to check bacterial growth and RFP rate synthesis in order to evaluate the R.P.U. (see Protocol) of the promoters from Anderson's collection in high copy and low copy number plasmids.

Methods

Accordign to Protocol 2 the experiment was performed in M9 on the following parts:

  • <partinfo>BBa_J23100</partinfo>
  • <partinfo>BBa_J23101</partinfo>
  • <partinfo>BBa_J23105</partinfo>
  • <partinfo>BBa_J23106</partinfo>
  • <partinfo>BBa_J23110</partinfo>
  • <partinfo>BBa_J23114</partinfo>
  • <partinfo>BBa_J23116</partinfo>
  • <partinfo>BBa_J23118</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23100</partinfo> (wiki name: BBa_J23100_4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23101</partinfo> (wiki name: BBa_J23101_4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23105</partinfo> (wiki name: BBa_J23105_4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23106</partinfo> (wiki name: BBa_J23106_4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23110</partinfo> (wiki name: BBa_J23110_4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23114</partinfo> (wiki name: BBa_J23114_4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23116</partinfo> (wiki name: BBa_J23116_4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23118</partinfo> (wiki name: BBa_J23118_4C5)
  • <partinfo>BBa_B0031</partinfo> (negative control)

RESULTS

UNIPV Pavia HCpromotersM9.png

In high copy number plasmids it is possible to identify 3 groups: strong promoters (<partinfo>Ba_J23100</partinfo> and <partinfo>BBa_J23101</partinfo>), medium strength promoters (in order <partinfo>BBa_J23110</partinfo> and <partinfo>BBa_J23118</partinfo>) and a group of weak promoters.

Promoter R.P.U. (average) std
<partinfo>BBa_J23100</partinfo> 0.946 0.182
<partinfo>BBa_J23101</partinfo> 1 -
<partinfo>BBa_J23105</partinfo> 0.196 0.038
<partinfo>BBa_J23106</partinfo> 0.326 0.015
<partinfo>BBa_J23110</partinfo> 0.678 0.096
<partinfo>BBa_J23114</partinfo> 0.091 0.022
<partinfo>BBa_J23116</partinfo> 0.101 0.004
<partinfo>BBa_J23118</partinfo> 0.655 0.035
UNIPV Pavia LCpromotersM9.png

In low copy number plasmids there are the same 3 groups but <partinfo>BBa_J23118</partinfo> resulted stronger than <partinfo>BBa_J23110</partinfo>.

Promoter R.P.U. (average) std
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23100</partinfo> 0.838 0.101
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23101</partinfo> 1 -
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23105</partinfo> 0.149 0.045
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23106</partinfo> 0.286 0.033
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23110</partinfo> 0.466 0.101
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23114</partinfo> 0.053 0.048
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23116</partinfo> 0.110 0.007
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23118</partinfo> 0.714 0.154



Previous Test Next Test

Tecan Test

Retrieved from "http://2010.igem.org/Team:UNIPV-Pavia/Material_Methods/Measurements/Tecan/test2settembre"