Team:Wisconsin-Madison/parts

From 2010.igem.org

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==New Standards==
==New Standards==
Wisconsin-Madison Team has taken the initiative to improve maintenance of the Registry of Standard Parts with more user experience in the form of zip files! Each fill will be labeled by its corresponding part number in the registry (ie. BBa_K318501) and will contain the following:
Wisconsin-Madison Team has taken the initiative to improve maintenance of the Registry of Standard Parts with more user experience in the form of zip files! Each fill will be labeled by its corresponding part number in the registry (ie. BBa_K318501) and will contain the following:
-
* Genbank including features and plasmid (.gbk???)
+
* Genbank including features and plasmid (.gbk)
* Fwd and Reverse sequencing files (.ab1)
* Fwd and Reverse sequencing files (.ab1)
* Gels from cloning and confirmation (.pdf)
* Gels from cloning and confirmation (.pdf)
* All associated testing files and Results (.xls, .pdf, etc.)
* All associated testing files and Results (.xls, .pdf, etc.)
 +
 +
Here is a copy of our submitted standard, BBF-RFC-64: [[Media:BBF_RFC_64.pdf]]
==Enzyme Delivery==
==Enzyme Delivery==
Line 48: Line 50:
|Regulatory - pH inducible promoter
|Regulatory - pH inducible promoter
|<partinfo>BBa_k318513</partinfo>
|<partinfo>BBa_k318513</partinfo>
-
|512
+
|[[media:BBa_K318512_pSB1C3.zip|here]]
|-
|-
|<partinfo>BBa_k318514</partinfo>
|<partinfo>BBa_k318514</partinfo>
|Gene - RamA protein can bind to cholic acid (bile salts)
|Gene - RamA protein can bind to cholic acid (bile salts)
|<partinfo>BBa_k318515</partinfo>
|<partinfo>BBa_k318515</partinfo>
-
|514
+
|[[Media:Zip_file_ramA.zip]]
|-
|-
|<partinfo>BBa_k318516</partinfo>
|<partinfo>BBa_k318516</partinfo>
|Regulatory - RamA/Bile-salt conjugate inducible promoter
|Regulatory - RamA/Bile-salt conjugate inducible promoter
|<partinfo>BBa_k318517</partinfo>
|<partinfo>BBa_k318517</partinfo>
-
|516
+
|[[Media:Zip_file_acrRA.zip]]
|}
|}
<br>
<br>
 +
===<center>Testing Constructs</center>===
===<center>Testing Constructs</center>===
{| border="1" cellpadding="5" cellspacing="0" align="center"
{| border="1" cellpadding="5" cellspacing="0" align="center"
Line 73: Line 76:
|Produces Trascription Factor RcsB
|Produces Trascription Factor RcsB
|Inducible - IPTG
|Inducible - IPTG
-
|500
+
|[[media:BBa_K318500_pSB1C3.zip|here]]
|-
|-
|<partinfo>BBa_k318501</partinfo>
|<partinfo>BBa_k318501</partinfo>
|Produces Trascription Factor RcsA
|Produces Trascription Factor RcsA
|Inducible - IPTG
|Inducible - IPTG
-
|501
+
|[[media:BBa_K318501_pSB1C3.zip|here]]
|-
|-
|<partinfo>BBa_k318502</partinfo>
|<partinfo>BBa_k318502</partinfo>
|Produces Trascription Factor RcsA & RcsB
|Produces Trascription Factor RcsA & RcsB
|Inducible - IPTG
|Inducible - IPTG
-
|502
+
|[[media:BBa_K318502_pSB1C3.zip|here]]
|-
|-
|<partinfo>BBa_k318503</partinfo>
|<partinfo>BBa_k318503</partinfo>
|Produces Trascription Factor YgiV - Blocks Biofilm
|Produces Trascription Factor YgiV - Blocks Biofilm
|Inducible - IPTG
|Inducible - IPTG
-
|503
+
|DNA Not Sent
|-
|-
|<partinfo>BBa_k318504</partinfo>
|<partinfo>BBa_k318504</partinfo>
|Produces RfaI gene - WaaI Ligase
|Produces RfaI gene - WaaI Ligase
|Inducible - IPTG
|Inducible - IPTG
-
|504
+
|DNA Not Sent
|-
|-
|<partinfo>BBa_k318505</partinfo>
|<partinfo>BBa_k318505</partinfo>
|Produces T.F. YgiV & RfaI gene
|Produces T.F. YgiV & RfaI gene
|Inducible - IPTG
|Inducible - IPTG
-
|505
+
|DNA Not Sent
|-
|-
|<partinfo>BBa_k318506</partinfo>
|<partinfo>BBa_k318506</partinfo>
|Produces RFP
|Produces RFP
|(+)LuxR, (-)cILVA
|(+)LuxR, (-)cILVA
-
|506
+
|[[media:BBa_K318506_pSB1C3.zip|here]]
|-
|-
|<partinfo>BBa_k318509</partinfo>
|<partinfo>BBa_k318509</partinfo>
|Produces Repressor cI-LVA (degredation tag)
|Produces Repressor cI-LVA (degredation tag)
|Inducible - IPTG
|Inducible - IPTG
-
|509
+
|DNA Not Sent
|-
|-
|<partinfo>BBa_k318510</partinfo>
|<partinfo>BBa_k318510</partinfo>
|Produces Repressor cI (no degredation tag)
|Produces Repressor cI (no degredation tag)
|Inducible - IPTG
|Inducible - IPTG
-
|510
+
|DNA Not Sent
|-
|-
|<partinfo>BBa_k318511</partinfo>
|<partinfo>BBa_k318511</partinfo>
|Produces Activator LuxR
|Produces Activator LuxR
|Anhydrous Tetrycycline
|Anhydrous Tetrycycline
-
|511
+
|[[media:BBa_K318511_pSB1C3.zip|here]]
|-
|-
|<partinfo>BBa_k318513</partinfo>
|<partinfo>BBa_k318513</partinfo>
|Produces RFP
|Produces RFP
|pH
|pH
-
|513
+
|[[media:BBa_K318513_pSB1C3.zip|here]]
|-
|-
|<partinfo>BBa_k318515</partinfo>
|<partinfo>BBa_k318515</partinfo>
-
|Produces Trascription Factor RcsA & RcsB
+
|Produces RFP
|RamA/Bile-Salt Complex
|RamA/Bile-Salt Complex
-
|515
+
|[[Media:Zip_file_acrRA.zip]]
|-
|-
|<partinfo>BBa_k318517</partinfo>
|<partinfo>BBa_k318517</partinfo>
|Produces RamA gene
|Produces RamA gene
|Bile Salt
|Bile Salt
-
|517
+
|[[Media:Zip_file_ramA.zip]]
|}
|}
<br>
<br>
 +
===<center>Final Constructs</center>===
===<center>Final Constructs</center>===
{| border="1" cellpadding="5" cellspacing="0" align="center"
{| border="1" cellpadding="5" cellspacing="0" align="center"
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|'''Expression Type'''
|'''Expression Type'''
|-
|-
-
|<partinfo>BBa_k3185##</partinfo>
+
|Not Cloned
|Produces Trascription Factors RcsA & RcsB, and B-Gal
|Produces Trascription Factors RcsA & RcsB, and B-Gal
|Constitutive
|Constitutive
|-
|-
-
|<partinfo>BBa_k3185##</partinfo>
+
|Not Cloned
|A) Produces equivalent ratio of Activator(LuxR) and Repressor(cI-LVA)
|A) Produces equivalent ratio of Activator(LuxR) and Repressor(cI-LVA)
|pH 2
|pH 2
|-
|-
|<partinfo>BBa_k318507</partinfo>
|<partinfo>BBa_k318507</partinfo>
-
|A) Activates Lysis once in the small intestine
+
|A) Activates Lysis once in the small intestine [[media:BBa_K318507_pSB1C3.zip|here]]
|(+)-LuxR, (-)-cILVA
|(+)-LuxR, (-)-cILVA
|-
|-
Line 169: Line 173:
|'''Part Number'''
|'''Part Number'''
|'''Description'''
|'''Description'''
-
|'''Testing in...'''
+
|'''Notes'''
|'''Zip File'''
|'''Zip File'''
|-
|-
|<partinfo>BBa_K318000</partinfo>
|<partinfo>BBa_K318000</partinfo>
|Key Cassette - produces Cre and Hin recombinases
|Key Cassette - produces Cre and Hin recombinases
-
|<partinfo>BBa_k318000</partinfo>
+
|Arabinose induces Cre expression, IPTG induces Hin expression
-
|[[Image:BBa_K318000_pSB1C3.zip]]
+
|[[Media:BBa_K318000_pSB1C3.zip]]
|-
|-
|<partinfo>BBa_K318030</partinfo>
|<partinfo>BBa_K318030</partinfo>
|Lock Cassette - prevents transcription unless "unlocked"
|Lock Cassette - prevents transcription unless "unlocked"
-
|<partinfo>BBa_k318515</partinfo>
+
|Requires successive Hin and Cre recombination for constitutive expression of downstream gene(s)
-
|030
+
|[[Media:BBa_K318030_pSB1C3.zip]]
|-
|-
-
|<partinfo>BBa_k318516</partinfo>
+
|<partinfo>BBa_K318521</partinfo>
-
|Regulatory - RamA/Bile-salt conjugate inducible promoter
+
|Lock Lysis Cassette - for use with iDIET
-
|<partinfo>BBa_k318517</partinfo>
+
|Requires successive pH then bile sensing by the organism to initiate cellular lysis.
-
|516
+
|Design phase only
|}
|}
<br>
<br>

Latest revision as of 03:16, 28 October 2010

Contents

New Standards

Wisconsin-Madison Team has taken the initiative to improve maintenance of the Registry of Standard Parts with more user experience in the form of zip files! Each fill will be labeled by its corresponding part number in the registry (ie. BBa_K318501) and will contain the following:

  • Genbank including features and plasmid (.gbk)
  • Fwd and Reverse sequencing files (.ab1)
  • Gels from cloning and confirmation (.pdf)
  • All associated testing files and Results (.xls, .pdf, etc.)

Here is a copy of our submitted standard, BBF-RFC-64: Media:BBF_RFC_64.pdf

Enzyme Delivery

New DNA

Enzyme Delivery Testing Constructs
Part Number About Testing in... Zip File
<partinfo>BBa_k318512</partinfo> Regulatory - pH inducible promoter <partinfo>BBa_k318513</partinfo> here
<partinfo>BBa_k318514</partinfo> Gene - RamA protein can bind to cholic acid (bile salts) <partinfo>BBa_k318515</partinfo> Media:Zip_file_ramA.zip
<partinfo>BBa_k318516</partinfo> Regulatory - RamA/Bile-salt conjugate inducible promoter <partinfo>BBa_k318517</partinfo> Media:Zip_file_acrRA.zip


Testing Constructs

Enzyme Delivery Testing Constructs
Part Number Function Expression Type Zip File
<partinfo>BBa_k318500</partinfo> Produces Trascription Factor RcsB Inducible - IPTG here
<partinfo>BBa_k318501</partinfo> Produces Trascription Factor RcsA Inducible - IPTG here
<partinfo>BBa_k318502</partinfo> Produces Trascription Factor RcsA & RcsB Inducible - IPTG here
<partinfo>BBa_k318503</partinfo> Produces Trascription Factor YgiV - Blocks Biofilm Inducible - IPTG DNA Not Sent
<partinfo>BBa_k318504</partinfo> Produces RfaI gene - WaaI Ligase Inducible - IPTG DNA Not Sent
<partinfo>BBa_k318505</partinfo> Produces T.F. YgiV & RfaI gene Inducible - IPTG DNA Not Sent
<partinfo>BBa_k318506</partinfo> Produces RFP (+)LuxR, (-)cILVA here
<partinfo>BBa_k318509</partinfo> Produces Repressor cI-LVA (degredation tag) Inducible - IPTG DNA Not Sent
<partinfo>BBa_k318510</partinfo> Produces Repressor cI (no degredation tag) Inducible - IPTG DNA Not Sent
<partinfo>BBa_k318511</partinfo> Produces Activator LuxR Anhydrous Tetrycycline here
<partinfo>BBa_k318513</partinfo> Produces RFP pH here
<partinfo>BBa_k318515</partinfo> Produces RFP RamA/Bile-Salt Complex Media:Zip_file_acrRA.zip
<partinfo>BBa_k318517</partinfo> Produces RamA gene Bile Salt Media:Zip_file_ramA.zip


Final Constructs

Enzyme Delivery Final Constructs
Part Number Function Expression Type
Not Cloned Produces Trascription Factors RcsA & RcsB, and B-Gal Constitutive
Not Cloned A) Produces equivalent ratio of Activator(LuxR) and Repressor(cI-LVA) pH 2
<partinfo>BBa_k318507</partinfo> A) Activates Lysis once in the small intestine here (+)-LuxR, (-)-cILVA
<partinfo>BBa_k318519</partinfo> B) Activates lysis once in the small intestine Bile Salts


Encryption

Encryption Constructs
Part Number Description Notes Zip File
<partinfo>BBa_K318000</partinfo> Key Cassette - produces Cre and Hin recombinases Arabinose induces Cre expression, IPTG induces Hin expression Media:BBa_K318000_pSB1C3.zip
<partinfo>BBa_K318030</partinfo> Lock Cassette - prevents transcription unless "unlocked" Requires successive Hin and Cre recombination for constitutive expression of downstream gene(s) Media:BBa_K318030_pSB1C3.zip
<partinfo>BBa_K318521</partinfo> Lock Lysis Cassette - for use with iDIET Requires successive pH then bile sensing by the organism to initiate cellular lysis. Design phase only


Other Parts

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K318010 BBa_K318010]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K318011 BBa_K318011]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K318012 BBa_K318012]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K318013 BBa_K318013]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K318014 BBa_K318014]

Retrieved from "http://2010.igem.org/Team:Wisconsin-Madison/parts"