Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test14settembre

From 2010.igem.org

(Difference between revisions)

Latest revision as of 21:26, 27 October 2010

SEPTEMBER, 14TH

PLATE

EXPERIMENT DESCRIPTION

Motivation

This experiment was performed in LB to check bacterial growth and RFP rate synthesis in order to evaluate the R.P.U. (see Protocol) of the promoters from Anderson's collection in low copy number plasmids.

Methods

Accordign to Protocol 2 the experiment was performed in LB on the following parts:

<partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23100</partinfo> (wiki name: BBa_J23100_4C5)
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23101</partinfo> (wiki name: BBa_J23101_4C5)
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23105</partinfo> (wiki name: BBa_J23105_4C5)
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23106</partinfo> (wiki name: BBa_J23106_4C5)
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23110</partinfo> (wiki name: BBa_J23110_4C5)
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23114</partinfo> (wiki name: BBa_J23114_4C5)
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23116</partinfo> (wiki name: BBa_J23116_4C5)
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23118</partinfo> (wiki name: BBa_J23118_4C5)
<partinfo>BBa_B0031</partinfo> (negative control)

RESULTS

Unfortunately it was not possbile to read properly the fluorescence that resulted too weak.

Tecan Test

@@ Line 11: / Line 11: @@
 <td valign="top">
 <table border="0" align="center" width="100%"><tr><td align="justify" valign="top" style="padding:20px">
-<html><p align="center"><font size="5"><b>18° TEST</b></font><br><br><font size="4">[SEPTEMBER, 14TH]</font></p></html><hr><br>
+<html><p align="center"><font size="5"><b>SEPTEMBER, 14TH</b></font></p></html><hr><br>
 ==PLATE==
 <br>
@@ Line 35: / Line 35: @@
 * <partinfo>BBa_B0031</partinfo> (negative control)
-==RESULTS (?)==
+==RESULTS==
+Unfortunately it was not possbile to read properly the fluorescence that resulted too weak.
+[[Image:UNIPV_Pavia_fluoLC.png| 620px | center ]]
 <!-- table previous next test -->
@@ Line 41: / Line 44: @@
 <table border="0" width="100%" class="menu">
 <tr>
-<td align="left" width="50%">[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test2settembre|Previous Test]]</td>
+<td align="left" width="50%">[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test10settembre_bis|Previous Test]]</td>
-<td align="right" width="50%">[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test15settembre| Next Test]]</td>
+<td align="right" width="50%">[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test16settembre| Next Test]]</td>
 </tr>
 </table>