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Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test2settembre
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<table border="0" align="center" width="100%"><tr><td align="justify" valign="top" style="padding:20px"> | <table border="0" align="center" width="100%"><tr><td align="justify" valign="top" style="padding:20px"> | ||
| - | <html><p align="center"><font size="5"><b> | + | <html><p align="center"><font size="5"><b>SEPTEMBER, 2ND</b></font></p></html><hr><br> |
| - | + | ||
==PLATE== | ==PLATE== | ||
<br> | <br> | ||
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==EXPERIMENT DESCRIPTION== | ==EXPERIMENT DESCRIPTION== | ||
| - | ==RESULTS ( | + | |
| + | ===Motivation=== | ||
| + | This experiment was performed in M9 to check bacterial growth and RFP rate synthesis in order to evaluate the R.P.U. ([[Team:UNIPV-Pavia/Parts/Characterization#Data analysis for RPU evaluation|see Protocol]]) of the promoters from Anderson's collection in high copy and low copy number plasmids. | ||
| + | |||
| + | ===Methods=== | ||
| + | Accordign to [[Team:UNIPV-Pavia/Parts/Characterization#Microplate reader experiments for constitutive promoters (R.P.U. evaluation) - Protocol #2|Protocol 2]] the experiment was performed in M9 on the following parts: | ||
| + | |||
| + | * <partinfo>BBa_J23100</partinfo> | ||
| + | * <partinfo>BBa_J23101</partinfo> | ||
| + | * <partinfo>BBa_J23105</partinfo> | ||
| + | * <partinfo>BBa_J23106</partinfo> | ||
| + | * <partinfo>BBa_J23110</partinfo> | ||
| + | * <partinfo>BBa_J23114</partinfo> | ||
| + | * <partinfo>BBa_J23116</partinfo> | ||
| + | * <partinfo>BBa_J23118</partinfo> | ||
| + | * <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23100</partinfo> (wiki name: BBa_J23100_4C5) | ||
| + | * <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23101</partinfo> (wiki name: BBa_J23101_4C5) | ||
| + | * <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23105</partinfo> (wiki name: BBa_J23105_4C5) | ||
| + | * <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23106</partinfo> (wiki name: BBa_J23106_4C5) | ||
| + | * <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23110</partinfo> (wiki name: BBa_J23110_4C5) | ||
| + | * <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23114</partinfo> (wiki name: BBa_J23114_4C5) | ||
| + | * <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23116</partinfo> (wiki name: BBa_J23116_4C5) | ||
| + | * <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23118</partinfo> (wiki name: BBa_J23118_4C5) | ||
| + | * <partinfo>BBa_B0031</partinfo> (negative control) | ||
| + | |||
| + | ==RESULTS== | ||
| + | |||
| + | [[Image:UNIPV_Pavia_HCpromotersM9.png| 620px | center ]] | ||
| + | |||
| + | In high copy number plasmids it is possible to identify 3 groups: strong promoters (<partinfo>Ba_J23100</partinfo> and <partinfo>BBa_J23101</partinfo>), medium strength promoters (in order <partinfo>BBa_J23110</partinfo> and <partinfo>BBa_J23118</partinfo>) and a group of weak promoters. | ||
| + | |||
| + | {| border='1' align='center' | ||
| + | || Promoter || R.P.U. (average) || std | ||
| + | |- | ||
| + | || <partinfo>BBa_J23100</partinfo> || 0.946 || 0.182 | ||
| + | |- | ||
| + | || <partinfo>BBa_J23101</partinfo> || 1 || - | ||
| + | |- | ||
| + | || <partinfo>BBa_J23105</partinfo> || 0.196 || 0.038 | ||
| + | |- | ||
| + | || <partinfo>BBa_J23106</partinfo> || 0.326 || 0.015 | ||
| + | |- | ||
| + | || <partinfo>BBa_J23110</partinfo> || 0.678 || 0.096 | ||
| + | |- | ||
| + | || <partinfo>BBa_J23114</partinfo> || 0.091 || 0.022 | ||
| + | |- | ||
| + | || <partinfo>BBa_J23116</partinfo> || 0.101 || 0.004 | ||
| + | |- | ||
| + | || <partinfo>BBa_J23118</partinfo> || 0.655 || 0.035 | ||
| + | |} | ||
| + | |||
| + | [[Image:UNIPV_Pavia_LCpromotersM9.png| 620px | center ]] | ||
| + | |||
| + | In low copy number plasmids there are the same 3 groups but <partinfo>BBa_J23118</partinfo> resulted stronger than <partinfo>BBa_J23110</partinfo>. | ||
| + | |||
| + | {| border='1' align='center' | ||
| + | || Promoter || R.P.U. (average) || std | ||
| + | |- | ||
| + | || <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23100</partinfo> || 0.838 || 0.101 | ||
| + | |- | ||
| + | || <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23101</partinfo> || 1 || - | ||
| + | |- | ||
| + | || <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23105</partinfo> || 0.149 || 0.045 | ||
| + | |- | ||
| + | || <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23106</partinfo> || 0.286 || 0.033 | ||
| + | |- | ||
| + | || <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23110</partinfo> || 0.466 || 0.101 | ||
| + | |- | ||
| + | || <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23114</partinfo> || 0.053 || 0.048 | ||
| + | |- | ||
| + | || <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23116</partinfo> || 0.110 || 0.007 | ||
| + | |- | ||
| + | || <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23118</partinfo> || 0.714 || 0.154 | ||
| + | |} | ||
<!-- table previous next test --> | <!-- table previous next test --> | ||
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<tr> | <tr> | ||
<td align="left" width="50%">[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test1settembre|Previous Test]]</td> | <td align="left" width="50%">[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test1settembre|Previous Test]]</td> | ||
| - | <td align="right" width="50%">[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/ | + | <td align="right" width="50%">[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test8settembre| Next Test]]</td> |
</tr> | </tr> | ||
</table> | </table> | ||
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<td align="left" valign="top" width="15%"> | <td align="left" valign="top" width="15%"> | ||
| - | + | {{UNIPV-Pavia/Menu_measurments}} | |
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</table> | </table> | ||
Latest revision as of 14:10, 27 October 2010
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