Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test8settembre
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- | <html><p align="center"><font size="5"><b> | + | <html><p align="center"><font size="5"><b>SEPTEMBER, 8TH</b></font></p></html><hr><br> |
==PLATE== | ==PLATE== | ||
<br> | <br> | ||
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===Methods=== | ===Methods=== | ||
+ | According to [[Team:UNIPV-Pavia/Parts/Characterization#Microplate reader experiments for 3OC6-HSL quantification by means of BBa_T9002 sensor - Protocol #3|Protocol 3]] the experiment was performed in LB on the following signalling parts: | ||
- | ==RESULTS | + | * <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300030</partinfo> (wiki name: I14) |
+ | * <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300028</partinfo> (wiki name: I15) | ||
+ | * <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300029</partinfo> (wiki name: I16) | ||
+ | * <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300025</partinfo> (wiki name: I17) | ||
+ | * <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300026</partinfo> (wiki name: I18) | ||
+ | * <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300027</partinfo> (wiki name: I19) | ||
+ | * <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> (wiki name: I14-4C5) | ||
+ | * <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> (wiki name: I15-4C5) | ||
+ | * <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> (wiki name: I16-4C5) | ||
+ | * <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> (wiki name: I17-4C5) | ||
+ | * <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> (wiki name: I18-4C5) | ||
+ | * <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> (wiki name: I19-4C5) | ||
+ | * <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0031</partinfo> (negative control) | ||
+ | * <partinfo>pSB1AK3</partinfo> - <partinfo>BBa_K300009</partinfo> (negative control) | ||
+ | |||
+ | The light-blue wells refer to the same cultures grown over night; as the results were the same of the cultures grown for about six hours, from now on we have followed only the steps shown in [[Team:UNIPV-Pavia/Parts/Characterization#Microplate reader experiments for self-inducible promoters - Protocol #3|Protocol 3]]. All supernatants were diluted 1:100. | ||
+ | |||
+ | ==RESULTS== | ||
+ | |||
+ | [[Image:UNIPV_Pavia_Serie18.png| 620px | center ]] | ||
+ | |||
+ | It was possible to evaluate some of the 3OC6-HSL concentrations: | ||
+ | |||
+ | {| border='1' align='center' | ||
+ | || Part || 3OC6-HSL concentration [uM] | ||
+ | |- | ||
+ | || <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300030</partinfo> || ~0.7 | ||
+ | |- | ||
+ | || <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300028</partinfo> || ~0.04 | ||
+ | |- | ||
+ | || <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300025</partinfo> || ~0.09 | ||
+ | |} | ||
+ | |||
+ | Because supernatants were not properly diluted it was not possible to measure some of the 3OC6-HSL concentrations; a more accurate evaluation of 3OC6-HSL was performed in the [[Team:UNIPV-Pavia/Material_Methods/Measurements/Tecan/test10settembre_bis|next test]]. | ||
<!-- table previous next test --> | <!-- table previous next test --> | ||
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Latest revision as of 09:55, 23 October 2010
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