Team:UNIPV-Pavia/Calendar/October
From 2010.igem.org
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<tr><th align="center" width="12%"><b>DATE</b></th><th align="center"><b>ACTIVITY</b></th></tr> | <tr><th align="center" width="12%"><b>DATE</b></th><th align="center"><b>ACTIVITY</b></th></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana5 #October, 1st|October, 1st]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana5 #October, 1st|October, 1st]]</td><td align="left"> | ||
- | + | 99° Bio-Lab - Trasformed plates were chacked(I70, I71, I72 and I73). | |
Transformation of I55 into E. coli DH5-alpha. It was plated on LB+Amp 100 agar plate and let grow ON at +37°C. | Transformation of I55 into E. coli DH5-alpha. It was plated on LB+Amp 100 agar plate and let grow ON at +37°C. | ||
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</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana5 #October, 2nd|October, 2nd]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana5 #October, 2nd|October, 2nd]]</td><td align="left"> | ||
- | + | 100° Bio-Lab - PCR from colony for I55 (eleven colonies were picked).We decided to make glycerol stock for I55-1. Inoculum into 5 ml LB+Amp of the positive culture that was let grow ON at 37°C, 220 rpm. | |
Miniprep and quantification for I73-1/2/3/4/5/6. 1,5 hours E-P digest (for screening) and gel run. | Miniprep and quantification for I73-1/2/3/4/5/6. 1,5 hours E-P digest (for screening) and gel run. | ||
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</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana5 #October, 3rd|October, 3rd]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana5 #October, 3rd|October, 3rd]]</td><td align="left"> | ||
- | + | 101° Bio-Lab - Glycerol stock for:I55-1, IC-1/2/3/4-4C5. | |
Pick of a single non-red colony of INTEIN_C3 and inoculum into 5 ml LB+Cm34. Sample was let grow ON at +37°C, 220 rpm. | Pick of a single non-red colony of INTEIN_C3 and inoculum into 5 ml LB+Cm34. Sample was let grow ON at +37°C, 220 rpm. | ||
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<tr><th align="center" width="12%" colspan="2"><b>OCTOBER: WEEK 1</b></th></tr> | <tr><th align="center" width="12%" colspan="2"><b>OCTOBER: WEEK 1</b></th></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 4th|October, 4th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 4th|October, 4th]]</td><td align="left"> | ||
- | + | 102° Bio-Lab - Miniprep and quantification of: INTEIN_C3, I74-8, I54, I35. Digest and gel run/cut/quantification of these samples. | |
Ligations of I78=I74+INTEIN_C3. | Ligations of I78=I74+INTEIN_C3. | ||
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</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 5th|October, 5th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 5th|October, 5th]]</td><td align="left"> | ||
- | + | 103° Bio-Lab - Digestions were performed again for I55, I37, I57, I31 and I54. Digestions were let at +37°C ON. | |
Transformation (1ul) of I78 into E. coli TOP10. In order to check our E. coli TOP10 competent cells we plated them on LB+Amp and Cm agar plates. | Transformation (1ul) of I78 into E. coli TOP10. In order to check our E. coli TOP10 competent cells we plated them on LB+Amp and Cm agar plates. | ||
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</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 6th|October, 6th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 6th|October, 6th]]</td><td align="left"> | ||
- | + | 104° Bio-Lab - E. coli TOP10 plated on LB+Amp agar plate showed red colonies!!! | |
Transformation of available ligations I75 and I77 (1ul) into E. coli TOP10. They were plated on LB+Amp agar plates and let grow ON at +37°C, 220 rpm. | Transformation of available ligations I75 and I77 (1ul) into E. coli TOP10. They were plated on LB+Amp agar plates and let grow ON at +37°C, 220 rpm. | ||
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</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 7th|October, 7th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 7th|October, 7th]]</td><td align="left"> | ||
- | + | 105° Bio-Lab - I78 screening gel run. Glycerol stock and miniprep for I78-3. | |
Colony PCR for screening of I79 (we pick eleven colonies). | Colony PCR for screening of I79 (we pick eleven colonies). | ||
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</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 8th|October, 8th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 8th|October, 8th]]</td><td align="left"> | ||
- | + | 106° Bio-Lab - Glycerol stock, miniprep and Nanodrop quantification of I79-2.This DNA was sent to BMR Genomics for sequencing. | |
<b>We are now moving all our parts to pSB1C3 plasmid</b>. | <b>We are now moving all our parts to pSB1C3 plasmid</b>. | ||
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 9th|October, 9th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 9th|October, 9th]]</td><td align="left"> | ||
- | + | 107° Bio-Lab -A new part was cloned with these ones, named I80. It is the basic part to build self-inducible promoters. | |
<b>We are now moving all our parts to pSB1C3 plasmid</b>. | <b>We are now moving all our parts to pSB1C3 plasmid</b>. | ||
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 10th|October, 10th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 10th|October, 10th]]</td><td align="left"> | ||
- | + | 108° Bio-Lab - Inoculum into 4 ml LB+Amp of: I75-1/2/3, I76-1/2, I77-1/2/3, I47, I48, I49. They were let grow at +37°C, 220 rpm. | |
</td></tr> | </td></tr> | ||
<tr><th align="center" width="12%" colspan="2"><b>OCTOBER: WEEK 2</b></th></tr> | <tr><th align="center" width="12%" colspan="2"><b>OCTOBER: WEEK 2</b></th></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 11th|October, 11th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 11th|October, 11th]]</td><td align="left"> | ||
- | + | 109° Bio-Lab - <b>We are now moving all our parts to pSB1C3 plasmid</b>.<br>Inoculum of MC42-A, MC43-A, MG42-B, MG43-A, MC43_flip-a,b,c, MG43_flip-a,b,c, MG1655.<br> | |
15° Meeting - Updating about Bio-Lab activity. Decisions about poster and presentation. | 15° Meeting - Updating about Bio-Lab activity. Decisions about poster and presentation. | ||
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 12th|October, 12th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 12th|October, 12th]]</td><td align="left"> | ||
- | + | 110° Bio-Lab - <b>We are now moving all our parts to pSB1C3 plasmid</b>. <br>Screening PCR. | |
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 13th|October, 13th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 13th|October, 13th]]</td><td align="left"> | ||
- | + | 111° Bio-Lab - <b>We are now moving all our parts to pSB1C3 plasmid</b>. | |
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 14th|October, 14th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 14th|October, 14th]]</td><td align="left"> | ||
- | + | 112° Bio-Lab - <b>We are now moving all our parts to pSB1C3 plasmid</b>. | |
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 15th|October, 15th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 15th|October, 15th]]</td><td align="left"> | ||
- | + | 113° Bio-Lab - <b>We are now moving all our parts to pSB1C3 plasmid</b>. | |
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 16th|October, 16th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 16th|October, 16th]]</td><td align="left"> | ||
- | + | 114° Bio-Lab - <b>We are now moving all our parts to pSB1C3 plasmid</b>. | |
</td></tr> | </td></tr> | ||
<tr><th align="center" width="12%" colspan="2"><b>OCTOBER: WEEK 3</b></th></tr> | <tr><th align="center" width="12%" colspan="2"><b>OCTOBER: WEEK 3</b></th></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 18th|October, 18th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 18th|October, 18th]]</td><td align="left"> | ||
- | + | 115° Bio-Lab - We have finally completed the parts transfer to pSB1C3 plasmid. We sent the parts to MIT. | |
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 19th|October, 19th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 19th|October, 19th]]</td><td align="left"> | ||
- | + | 116° Bio-Lab - Wiki update and definition of results. | |
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 20th|October, 20th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 20th|October, 20th]]</td><td align="left"> | ||
- | + | 117° Bio-Lab - Wiki update and definition of results. | |
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 21st|October, 21st]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 21st|October, 21st]]</td><td align="left"> | ||
- | + | 118° Bio-Lab - Wiki update and definition of results. | |
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 22nd|October, 22nd]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 22nd|October, 22nd]]</td><td align="left"> | ||
- | + | 119° Bio-Lab - Wiki update and definition of results. | |
+ | <br>16°Meeting - Updating about biolab activity and definition of last details. | ||
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 23rd|October, 23rd]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 23rd|October, 23rd]]</td><td align="left"> | ||
- | + | 120° Bio-Lab - Wiki update and definition of results. | |
</td></tr> | </td></tr> | ||
<tr><th align="center" width="12%" colspan="2"><b>OCTOBER: WEEK 4</b></th></tr> | <tr><th align="center" width="12%" colspan="2"><b>OCTOBER: WEEK 4</b></th></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana4 #October, 25th|October, 25th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana4 #October, 25th|October, 25th]]</td><td align="left"> | ||
- | + | 121° Bio-Lab - Wiki update and definition of results | |
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana4 #October, 26th|October, 26th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana4 #October, 26th|October, 26th]]</td><td align="left"> | ||
- | + | 122° Bio-Lab - Wiki update and definition of results | |
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana4 #October, 27th|October, 27th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana4 #October, 27th|October, 27th]]</td><td align="left"> | ||
- | + | 123° Bio-Lab - Wiki update and definition of results | |
</td></tr> | </td></tr> | ||
Latest revision as of 18:18, 27 October 2010
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