Team:DTU-Denmark

The aim of this project is to engineer a genetic bistable switch that produces two different, mutually exclusive outputs when given two different inputs. The switch is based on the repressor-anti-repressor system of the Salmonella phages Gifsy1 and Gifsy2 and the λ-phage anti-termination system. The latest induced output will remain stable through generations, even once the input ceases, due to the phage regulatory systems.

We present the framework for this development and we characterize the regulatory mechanisms by using fluorescent proteins as outputs. The dynamics of the system will be modeled and we will also attempt to characterize and submit the promoters, repressors and anti-repressors from the Salmonella phages, as well as the two anti-terminator proteins from the lambda phage, as BioBricks.

There are multiple potential applications for a biological "switch" like ours in the world of biotechnology i.e. within the medical and environmental field.

**Figure 1** Fun in the lab!!

Quick Update

October 24th, 2010

The structure of our wiki has been changed slightly... Our Modeling section can now be found under The Project.

October 15th, 2010

Maya, Thomas and Juliet took the BioLector for a ride! And it was cool way to spend our Friday evening ;D

October 7th, 2010

The DTU-iGEM team will be meeting up with the SDU-iGEM team this Saturday (9th Oct) give each other feedback on our projects and to have a social event!

October 1st, 2010

Check out our active blog: now with picture of the day!

August 14th, 2010

Pictures have been posted so check out our picture gallery!

Comments or questions to the team? Please Email us

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      <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2010.igem.org/Team:DTU-Denmark/Project" >The Project</a> </font></td>
      <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2010.igem.org/Team:DTU-Denmark/Parts" >Parts submitted</a> </font></td>
-     <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2010.igem.org/Team:DTU-Denmark/Modelling">Modelling</a></font> </td>
+     <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2010.igem.org/Team:DTU-Denmark/Results">Results</a></font> </td>
      <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2010.igem.org/Team:DTU-Denmark/Notebook" title="Day to day lab activity">Notebook</a>
     <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2010.igem.org/Team:DTU-Denmark/Blog">Blog</a></font> </td>
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      Sponsors and acknowledgements<br><br>
     <font color="DDDDDD" face="arial" size="2">
-     <a href="https://2010.igem.org/Team:DTU-Denmark/sponsors" CLASS=leftbar>- List of sponsors</a><br>
+<ul>
-     <a href="https://2010.igem.org/Team:DTU-Denmark/Acknowledgement" CLASS=leftbar>- Acknowledgements</a><br>
+     <li><a href="https://2010.igem.org/Team:DTU-Denmark/sponsors" CLASS=leftbar>List of sponsors</a></li>
+     <li><a href="https://2010.igem.org/Team:DTU-Denmark/Acknowledgement" CLASS=leftbar>Acknowledgements and Contributions</a></li>
+    <li><a href="https://2010.igem.org/Team:DTU-Denmark/Collaborations" CLASS=leftbar>Collaborations</a></li>
+</ul>
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     </font>
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-     <a href="http://www.bio.dtu.dk/igem.aspx" target="_blank" CLASS=leftbar>- DTU iGEM 2010</a><br>
+<ul>
+     <li><a href="http://www.bio.dtu.dk/igem.aspx" target="_blank" CLASS=leftbar>DTU iGEM 2010</a></li>
+</ul>
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-     <a href="http://www.facebook.com/pages/iGEM-2010-DTU/134540923224394?ref=ts" target="_blank" CLASS=leftbar>- Facebook</a><br>
+<ul>
-   </font>
+     <li><a href="http://www.facebook.com/pages/iGEM-2010-DTU/134540923224394?ref=ts" target="_blank" CLASS=leftbar>Facebook</a></li>
-   <font color="DDDDDD" face="arial" size="2">
+    <li><a href="http://twitter.com/igemdtu2010" target="_blank" CLASS=leftbar>Twitter</a></li>
-    <a href="http://twitter.com/igemdtu2010" target="_blank" CLASS=leftbar>- Twitter</a><br>
+</ul>
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     </font>
     <font color="DDDDDD" face="arial" size="2">
-     <a href="https://2010.igem.org/Team:DTU-Denmark/Gallery">Picture Gallery</a>
+<ul>
+     <li><a href="https://2010.igem.org/Team:DTU-Denmark/Gallery">Photo Gallery</a></li>
+</ul>
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 <b>DTU DENMARK</b><br><br>
    </font>
-  <font color="#333333" face="arial" size="2.5">
+<p align="justify">The aim of this project is to engineer a genetic bistable switch that produces two different, mutually exclusive outputs when given two different inputs. The switch is based on the repressor-anti-repressor system of the Salmonella phages Gifsy1 and Gifsy2 and the λ-phage anti-termination system. The latest induced output will remain stable through generations, even once the input ceases, due to the phage regulatory systems.</p>
+<p align="justify">We present the framework for this development and we characterize the regulatory mechanisms by using fluorescent proteins as outputs. The dynamics of the system will be modeled and we will also attempt to characterize and submit the promoters, repressors and anti-repressors from the Salmonella phages, as well as the two anti-terminator proteins from the lambda phage, as BioBricks.</p>
+<p align="justify">There are multiple potential applications for a biological "switch" like ours in the world of biotechnology i.e. within the medical and environmental field.</p>
-<!-- INSERT MAIN TEXT HERE! (formatting: <b>bold</> <i>italic> <h4>header</h4>) -->
+<table class="https://static.igem.org/mediawiki/2010/e/ec/DTU_2biostable.png" align="center">
+ <caption align="bottom"><p align="justify"><b>Figure 1</b> Fun in the lab!!</p></caption>
-<p align="justify">The aim of this project is to engineer a genetic bi-stable switch that produces two different, mutually exclusive outputs when given two different inputs. The switch is based on the repressor-anti-repressor system of the salmonella phages Gifsy1 and Gifsy2 and the λ-phage anti-termination system. The latest induced output will remain stable through generations, even once the input ceases, due to the phage regulatory systems.</p>
+<tr><td><img src="https://static.igem.org/mediawiki/2010/e/ec/DTU_2biostable.png"  width="300px"></td></tr>
-<p align="justify">We present the framework for this development and characterize the regulatory mechanisms by using fluorescent proteins as the reporter (outputs). The dynamics of the system will be modeled and we will also attempt to characterize and submit the promoters, repressors and anti-repressors from the salmonella phages, as well as the two anti-terminator proteins from the lambda phage, as BioBricks.</p>
+</table><br>
-<h3>Basic Concept</h3>
+<table align="center">
-<img src="https://static.igem.org/mediawiki/2010/f/ff/DTU_Project_illustration_1.png" width="570px"  align="center"> </img>
+<tr>
-<p align="left"><font size="1.5"><b>Figure 1</b>: The basic concept of our project is illustrated:<br><i>Inducer 1</i> will activate <i>Promoter 1</i> thereby start up the production of <i>Repressor 1</i>, switching off the other part of our switch by repressing <i>Promoter 2</i>. This will essentially make the cells change from <font color="#990000">red</font color> to <font color="#33FF00">green</font color>! This means that when the cells are exposed to <i>Inducer 2</i>, the cell culture will turn from <font color="#33FF00">green</font color> to <font color="#990000">red</font color>.</font></p><br>
+<td align="center">
-  </font>
+<table class="https://static.igem.org/mediawiki/2010/4/47/Lundbeckfonden.gif" align="center">
+<tr><td><img src="https://static.igem.org/mediawiki/2010/4/47/Lundbeckfonden.gif"  width="150px"></td></tr>
+</table><br>
+</td>
+<td align="center">
+<table class="https://static.igem.org/mediawiki/2010/9/93/DTU_Corrit_fonden.png" align="center">
+<tr><td><img src="https://static.igem.org/mediawiki/2010/9/93/DTU_Corrit_fonden.png"  width="150px"></td></tr>
+</table><br>
+</td>
+<td align="center">
+<table class="https://static.igem.org/mediawiki/2010/8/80/DTUlogo.png" align="center">
+<tr><td><img src="https://static.igem.org/mediawiki/2010/8/80/DTUlogo.png" ></td></tr>
+</table><br>
+</tr>
+</table>
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 <font size="3"><strong>Quick Update</strong></font><br>
 <br>
+<strong>October 24th, 2010</strong><br>
+<p>The structure of our wiki has been changed slightly... Our <b><a href="https://2010.igem.org/Team:DTU-Denmark/Modelling"  target="_blank">Modeling</a></b> section can now be found under <b><a href="https://2010.igem.org/Team:DTU-Denmark/Project"  target="_blank">The Project</a></b>.<br>
+<br>
+</p>
 <strong>October 15th, 2010</strong><br>