"
Team:UNIPV-Pavia/Calendar/October/settimana3
From 2010.igem.org
(Difference between revisions)
(→October, 18th) |
(→October, 19th) |
||
| (20 intermediate revisions not shown) | |||
| Line 34: | Line 34: | ||
==October, 18th== | ==October, 18th== | ||
| - | We have finally completed the parts | + | We have finally completed the parts transfer to <partinfo>pSB1C3</partinfo> plasmid. |
All the parts that will be sent to the registry were previously screened by UNIPV-Pavia TEAM, as reported in this notebook and all of them were sequenced and sequencing results were correct. | All the parts that will be sent to the registry were previously screened by UNIPV-Pavia TEAM, as reported in this notebook and all of them were sequenced and sequencing results were correct. | ||
After parts have been moved to the shipping plasmid and checked by agarose gel electrophoresis. | After parts have been moved to the shipping plasmid and checked by agarose gel electrophoresis. | ||
| - | The parts we sent to MIT are: | + | The parts we sent to MIT and submitted to the registry are: |
<table border='1' align='center'> | <table border='1' align='center'> | ||
<tr><th>Part name</th><th>Wiki name</th><th>Plasmid</th><th>Number</th></tr> | <tr><th>Part name</th><th>Wiki name</th><th>Plasmid</th><th>Number</th></tr> | ||
| - | <tr><td><partinfo> | + | <tr><td><partinfo>BBa_K300005</partinfo></td><td>I0</td><td>pSB1C3</td><td>1</td></tr> |
| - | <tr><td><partinfo> | + | <tr><td><partinfo>BBa_K300006</partinfo></td><td>I1</td><td>pSB1C3</td><td>2</td></tr> |
| - | <tr><td><partinfo> | + | <tr><td><partinfo>BBa_K300009</partinfo></td><td>I3</td><td>pSB1C3</td><td>3</td></tr> |
| - | <tr><td><partinfo> | + | <tr><td><partinfo>BBa_K300008</partinfo></td><td>I5</td><td>pSB1C3</td><td>4</td></tr> |
| - | <tr><td><partinfo> | + | <tr><td><partinfo>BBa_K300024</partinfo></td><td>I7</td><td>pSB1C3</td><td>5</td></tr> |
| - | <tr><td><partinfo> | + | <tr><td><partinfo>BBa_K300019</partinfo></td><td>I12</td><td>pSB1C3</td><td>6</td></tr> |
| - | <tr><td><partinfo> | + | <tr><td><partinfo>BBa_K300030</partinfo></td><td>I14</td><td>pSB1C3</td><td>7</td></tr> |
| - | <tr><td><partinfo> | + | <tr><td><partinfo>BBa_K300028</partinfo></td><td>I15</td><td>pSB1C3</td><td>8</td></tr> |
| - | <tr><td><partinfo> | + | <tr><td><partinfo>BBa_K300029</partinfo></td><td>I16</td><td>pSB1C3</td><td>9</td></tr> |
| - | <tr><td><partinfo> | + | <tr><td><partinfo>BBa_K300025</partinfo></td><td>I17</td><td>pSB1C3</td><td>10</td></tr> |
| - | <tr><td><partinfo> | + | <tr><td><partinfo>BBa_K300026</partinfo></td><td>I18</td><td>pSB1C3</td><td>11</td></tr> |
| - | <tr><td><partinfo> | + | <tr><td><partinfo>BBa_K300027</partinfo></td><td>I19</td><td>pSB1C3</td><td>12</td></tr> |
| - | <tr><td><partinfo> | + | <tr><td><partinfo>BBa_K300002</partinfo></td><td>I20</td><td>pSB1C3</td><td>13</td></tr> |
| - | <tr><td><partinfo> | + | <tr><td><partinfo>BBa_K300003</partinfo></td><td>I21</td><td>pSB1C3</td><td>14</td></tr> |
| - | <tr><td><partinfo> | + | <tr><td><partinfo>BBa_K300031</partinfo></td><td>I22</td><td>pSB1C3</td><td>15</td></tr> |
| - | <tr><td><partinfo> | + | <tr><td><partinfo>BBa_K300081</partinfo></td><td>I26</td><td>pSB1C3</td><td>16</td></tr> |
| - | <tr><td><partinfo> | + | <tr><td><partinfo>BBa_K300079</partinfo></td><td>I31</td><td>pSB1C3</td><td>17</td></tr> |
| - | <tr><td><partinfo> | + | <tr><td><partinfo>BBa_K300080</partinfo></td><td>I35</td><td>pSB1C3</td><td>18</td></tr> |
| - | <tr><td><partinfo> | + | <tr><td><partinfo>BBa_K300086</partinfo></td><td>I47</td><td>pSB1C3</td><td>19</td></tr> |
| - | <tr><td><partinfo> | + | <tr><td><partinfo>BBa_K300088</partinfo></td><td>I48</td><td>pSB1C3</td><td>20</td></tr> |
| - | <tr><td><partinfo> | + | <tr><td><partinfo>BBa_K300090</partinfo></td><td>I49</td><td>pSB1C3</td><td>21</td></tr> |
| - | <tr><td><partinfo> | + | <tr><td><partinfo>BBa_K300082</partinfo></td><td>I55</td><td>pSB1C3</td><td>22</td></tr> |
| - | <tr><td><partinfo> | + | <tr><td><partinfo>BBa_K300084</partinfo></td><td>I78</td><td>pSB1C3</td><td>23</td></tr> |
| - | <tr><td><partinfo> | + | <tr><td><partinfo>BBa_K300083</partinfo></td><td>I79</td><td>pSB1C3</td><td>24</td></tr> |
| - | <tr><td><partinfo> | + | <tr><td><partinfo>BBa_K300010</partinfo></td><td>I80</td><td>pSB1C3</td><td>25</td></tr> |
| - | <tr><td><partinfo> | + | <tr><td><partinfo>BBa_K300007</partinfo></td><td>I70 - BioBrick</td><td><partinfo>BBa_K300001</partinfo></td><td>26</td></tr> |
| - | <tr><td><partinfo> | + | <tr><td><partinfo>BBa_K300007</partinfo></td><td>I70 - vector</td><td><partinfo>BBa_K300001</partinfo></td><td>27</td></tr> |
| - | <tr><td><partinfo> | + | <tr><td><partinfo>BBa_K300004</partinfo></td><td>INTEIN</td><td>pSB1C3</td><td>28</td></tr> |
| - | <tr><td><partinfo> | + | <tr><td><partinfo>BBa_I52002</partinfo></td><td>I51</td><td><partinfo>BBa_K300000</partinfo></td><td>29</td></tr> |
| + | </table> | ||
| + | |||
| + | The parts we have in freezer and we only submitted to the Registry are: | ||
| + | <table border='1' align='center'> | ||
| + | <tr><th>Part name</th><th>Wiki name</th><th>Plasmid</th></tr> | ||
| + | <tr><td><partinfo>BBa_K300021</partinfo></td><td>I8</td><td><partinfo>BBa_J61002</partinfo></td></tr> | ||
| + | <tr><td><partinfo>BBa_K300022</partinfo></td><td>I9</td><td><partinfo>BBa_J61002</partinfo></td></tr> | ||
| + | <tr><td><partinfo>BBa_K300023</partinfo></td><td>I10</td><td><partinfo>BBa_J61002</partinfo></td></tr> | ||
| + | <tr><td><partinfo>BBa_K300091</partinfo></td><td>I63</td><td><partinfo>pSB1AK3</partinfo></td></tr> | ||
| + | <tr><td><partinfo>BBa_K300092</partinfo></td><td>I64</td><td><partinfo>pSB1A2</partinfo></td></tr> | ||
| + | <tr><td><partinfo>BBa_K300099</partinfo></td><td>I65</td><td><partinfo>pSB1A2</partinfo></td></tr> | ||
| + | <tr><td><partinfo>BBa_K300980</partinfo></td><td>YEAST MR.G</td><td>pMA commercial vector</td></tr> | ||
| + | <tr><td><partinfo>BBa_K300982</partinfo></td><td>CREAM</td><td>-</td></tr> | ||
| + | <tr><td><partinfo>BBa_K300983</partinfo></td><td>CRIM MR.G</td><td>pMK-RQ commercial vector</td></tr> | ||
| + | <!--tr><td><partinfo></partinfo></td><td></td><td><partinfo></partinfo></td></tr--> | ||
</table> | </table> | ||
| + | |||
| + | ---- | ||
| Line 80: | Line 97: | ||
==October, 19th== | ==October, 19th== | ||
| + | <b>Wiki update and definition of results.</b> | ||
| + | |||
| + | ---- | ||
| + | Every clones of E. coli genome integration project were amplified with PCR to verify their lengths and their positions into the genome. | ||
| + | In order to obtain better results we perform all the amplifications using an annealing temperature of 63°C. | ||
| + | Screening PCR was performed on MC42(A-B-C), MC43(A-B-C), MG42(B-C), MG43(A-B-C), MC1061 and MG1655. Primers used were BBa_K300975-BBa_K300976 (P1-P2), BBa_K300976-BBa_K300977 (P2-P3), BBa_K300977-BBa_K300978 (P3-P4). | ||
| + | |||
| + | [[Image:UNIPV10_19_10_10_MC42ABC_MC43ABC_MG42BC_MG43ABC_BLANK_MC1061_MG1655(TUTTI P1-P2).jpg|thumb|300px|center|The order of the samples was the follow: MC42(A,B,C), MC43(A,B,C), MG42(B,C), MG43(A,B,C), blank, MC1061, MG1655. Primers were BBa_K300975-BBa_K300976 (P1-P2).]] | ||
| + | |||
| + | [[Image:UNIPV10_19_10_10_MC42ABC_MC43ABC_MG42BC_MG43ABC_BLANK_MC1061_MG1655(TUTTI P2-P3).jpg|thumb|300px|center|The order of the samples was the follow: MC42(A,B,C), MC43(A,B,C), MG42(B,C), MG43(A,B,C), blank, MC1061, MG1655. Primers were BBa_K300976-BBa_K300977 (P2-P3).]] | ||
| + | |||
| + | [[Image:UNIPV10_19_10_10_MC42ABC_MC43ABC_MG42BC_MG43ABC_BLANK_MC1061_MG1655(TUTTI P3-P4).jpg|thumb|300px|center|The order of the samples was the follow: MC42(A,B,C), MC43(A,B,C), MG42(B,C), MG43(A,B,C), blank, MC1061, MG1655. Primers were BBa_K300977-BBa_K300978 (P3-P4).]] | ||
| + | |||
| + | |||
| + | |||
<div align="right"><small>[[#indice|^top]]</small></div> | <div align="right"><small>[[#indice|^top]]</small></div> | ||
==October, 20th== | ==October, 20th== | ||
| - | + | <b>Wiki update and definition of results.</b> | |
<div align="right"><small>[[#indice|^top]]</small></div> | <div align="right"><small>[[#indice|^top]]</small></div> | ||
==October, 21st== | ==October, 21st== | ||
| - | + | <b>Wiki update and definition of results.</b> | |
<div align="right"><small>[[#indice|^top]]</small></div> | <div align="right"><small>[[#indice|^top]]</small></div> | ||
==October, 22nd== | ==October, 22nd== | ||
| - | + | <b>Wiki update and definition of results.</b> | |
<div align="right"><small>[[#indice|^top]]</small></div> | <div align="right"><small>[[#indice|^top]]</small></div> | ||
==October, 23rd== | ==October, 23rd== | ||
| - | + | <b>Wiki update and definition of results.</b> | |
<div align="right"><small>[[#indice|^top]]</small></div> | <div align="right"><small>[[#indice|^top]]</small></div> | ||
Latest revision as of 10:09, 25 October 2010
|
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
.jpg){kind=spacer}
.jpg){kind=spacer}
.jpg){kind=spacer}
