Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/12

From 2010.igem.org

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{{:Team:Tokyo_Metropolitan/Notebook/Pattern}}
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=2010/10/12=
=2010/10/12=
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===1:DNA extraction===
===1:DNA extraction===
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<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
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===2:DNA Digestion===
===2:DNA Digestion===
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<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]
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===3:PCR===
===3:PCR===
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<Sample><br />
<Sample><br />
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*9
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9]
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*1-2-3
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2-3]
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*4-5
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5]
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ]
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===4:Plasmid extraction from E-coli===
===4:Plasmid extraction from E-coli===
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<Sample><br />
<Sample><br />
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*1-2
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2]
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*1-3
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-3]
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*1-5
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-5]
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*1-6
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-6]
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Plasmid_extraction_from_E-coli/ Protocol 6 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Plasmid_extraction_from_E-coli/ Protocol 6 ]
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===5:DNA Ligation ===
===5:DNA Ligation ===
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<Sample><br />
<Sample><br />
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*1-2   
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2]  
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*10-2  
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2]
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<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ]
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===3:DNA Digestion===
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===6:DNA Digestion===
<Member><br />
<Member><br />
nito
nito
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<Sample, Materials><br />
<Sample, Materials><br />
・PCR productions
・PCR productions
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*9H
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9H]
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*1-2
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2]
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*10-2
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2]
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・Digest enzyme
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*AvrⅡ
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*NheⅠ
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*SpeⅠ
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<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Protocol 9 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Protocol 9 ]

Latest revision as of 02:59, 25 October 2010


E.coli Pattern Formation Project Notebook



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Contents

2010/10/12

1:DNA extraction

<Member>
Mariko

<Sample>

<Protocol>
See Protocol 4


2:DNA Digestion

<Member>
Mariko

<Sample, Materials>
・PCR product

<Protocol>
See Prptpcpl 9


3:PCR

<Member>
nito

<Sample>

<Protocol>
See Protocol 2


4:Plasmid extraction from E-coli

<Member>
Takahiro

<Sample>

<Protocol>
See Protocol 6


5:DNA Ligation

<Member>
nito

<Sample>

<Protocol>
See Protocol 10


6:DNA Digestion

<Member>
nito

<Sample, Materials>
・PCR productions

<Protocol>
See Protocol 9