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Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/12
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=2010/10/12= | =2010/10/12= | ||
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===1：DNA extraction=== | ===1：DNA extraction=== | ||
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＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ] | ||
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===2：DNA Digestion=== | ===2：DNA Digestion=== | ||
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＜Sample, Materials＞<br /> | ＜Sample, Materials＞<br /> | ||
・PCR product | ・PCR product | ||
| - | *1-2 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2] |
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＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ] | ||
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===3:PCR=== | ===3:PCR=== | ||
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<Sample><br /> | <Sample><br /> | ||
| - | *9 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9] |
| - | *1-2-3 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2-3] |
| - | *4-5 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5] |
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ] | ||
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===4:Plasmid extraction from E-coli=== | ===4:Plasmid extraction from E-coli=== | ||
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<Sample><br /> | <Sample><br /> | ||
| - | *1-2 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2] |
| - | *1-3 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-3] |
| - | *1-5 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-5] |
| - | *1-6 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-6] |
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Plasmid_extraction_from_E-coli/ Protocol 6 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Plasmid_extraction_from_E-coli/ Protocol 6 ] | ||
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===5：DNA Ligation === | ===5：DNA Ligation === | ||
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＜Sample＞<br /> | ＜Sample＞<br /> | ||
| - | *1-2 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2] |
| - | *10-2 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2] |
| - | + | ||
＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ] | ||
| - | === | + | |
| + | ===6：DNA Digestion=== | ||
＜Member＞<br /> | ＜Member＞<br /> | ||
nito | nito | ||
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＜Sample, Materials＞<br /> | ＜Sample, Materials＞<br /> | ||
・PCR productions | ・PCR productions | ||
| - | *9H | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9H] |
| - | *1-2 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2] |
| - | *10-2 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2] |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Protocol 9 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Protocol 9 ] | ||
Latest revision as of 02:59, 25 October 2010
E.coli Pattern Formation Project Notebook
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Contents |
2010/10/12
1：DNA extraction
＜Member＞
Mariko
＜Sample＞
＜Protocol＞
See Protocol 4
2：DNA Digestion
＜Member＞
Mariko
＜Sample, Materials＞
・PCR product
＜Protocol＞
See Prptpcpl 9
3:PCR
<Member>
nito
<Sample>
<Protocol>
See Protocol 2
4:Plasmid extraction from E-coli
<Member>
Takahiro
<Sample>
<Protocol>
See Protocol 6
5：DNA Ligation
＜Member＞
nito
＜Sample＞
＜Protocol＞
See Protocol 10
6：DNA Digestion
＜Member＞
nito
＜Sample, Materials＞
・PCR productions
＜Protocol＞
See Protocol 9
