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Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/08
From 2010.igem.org
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| - | {{:Team:Tokyo_Metropolitan/ | + | {{:Team:Tokyo_Metropolitan/Notebook/Pattern}} |
| - | =2010/10/= | + | |
| + | =2010/10/08= | ||
===1:PCR①=== | ===1:PCR①=== | ||
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<Sample><br /> | <Sample><br /> | ||
PCR products | PCR products | ||
| - | *9 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9] |
| - | * | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2] |
| - | * | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2-10] |
<Protocol><br /> | <Protocol><br /> | ||
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＜Sample＞<br /> | ＜Sample＞<br /> | ||
| - | * | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2] |
| - | * | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5] |
| - | * | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2-10] |
(after digestion) | (after digestion) | ||
＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ] | ||
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===4:PCR②=== | ===4:PCR②=== | ||
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<Sample>(temperature in annealing:℃)<br /> | <Sample>(temperature in annealing:℃)<br /> | ||
| - | *7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7] |
| - | *9 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9] |
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ] | ||
| - | |||
===5：DNA extraction from gels=== | ===5：DNA extraction from gels=== | ||
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＜Sample＞<br /> | ＜Sample＞<br /> | ||
Digestion products | Digestion products | ||
| - | * | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2] |
| - | * | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2-10] |
(After electrophoresis) | (After electrophoresis) | ||
＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ] | ||
Latest revision as of 02:57, 25 October 2010
E.coli Pattern Formation Project Notebook
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Contents |
2010/10/08
1:PCR①
<Member>
Hitomi
<Sample>(temperature in annealing:℃)
<Protocol>
See Protocol 1
2:Electrophoresis
<Member>
nito
<Sample>
PCR products
<Protocol>
See Protocol 8
<Result>
3：DNA Ligation
＜Member＞
Hitomi
＜Sample＞
(after digestion)
＜Protocol＞
See Protocol 10
4:PCR②
<Member>
nito
<Sample>(temperature in annealing:℃)
<Protocol>
See Protocol 1
5：DNA extraction from gels
＜Member＞
nito
＜Sample＞
Digestion products
(After electrophoresis)
＜Protocol＞
See Protocol 4
