Team:Michigan

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Welcome to the homepage of the University of Michigan Synthetic Biology Team!
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==Project Abstract==
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'''Algae Bioflocculation for Biofuel Production and Bioremediation of Oil Sands Tailings Water'''
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==Project Background==
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Our team worked on two projects this year. Our first project aims to improve the economics of algal biofuel production by creating a cost efficient microalgae bioflocculant out of E. coli. To achieve this, we over-express Type I pili to increase the cell’s adhesiveness, and also express a chlorovirus protein on the cell surface which specifically binds Chlorella species, a promising algal feedstock for the biofuel industry.
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The project the team has decided on for this year is in association with the Chemical Engineering department’s joint project to produce hydrocarbons from algae biomass.  This consists of hydrothermal processing of the algae followed by catalytic upgrading of the “bio crude” oil.  Before the hydrothermal processing can take place the algae needs to be concentrated from 1 to 10 g/L to over 250 g/L. Traditional methods that are used like centrifugation, filtration and chemical flocculation are energy intensive. We propose creating a bioflocculation circuit in E. coli to flocculate the algae as an alternative option. This consists of a “trigger” for flocculation which would then express proteins that cause the surface of the E. coli cells to become sticky and flocculate with the algae.
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We are also participating in the Oil Sands Initiative and seeking to improve the biodegradation rate of naphthenic acids (NAs), a toxic by-product of the oil extraction process which can linger in the environment for decades. Two Pseudomonas strains have been found to synergistically degrade 95% of NAs. Our project focuses on engineering these Pseudomonas strains to form biofilms in the harsh tailings water environment, which can potentially increase degradation rates by two orders of magnitude, by expressing a self-associating E. coli protein.
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Latest revision as of 17:50, 27 October 2010


Michigan Header




Welcome to the homepage of the University of Michigan Synthetic Biology Team!

Project Abstract

Algae Bioflocculation for Biofuel Production and Bioremediation of Oil Sands Tailings Water

Our team worked on two projects this year. Our first project aims to improve the economics of algal biofuel production by creating a cost efficient microalgae bioflocculant out of E. coli. To achieve this, we over-express Type I pili to increase the cell’s adhesiveness, and also express a chlorovirus protein on the cell surface which specifically binds Chlorella species, a promising algal feedstock for the biofuel industry.

We are also participating in the Oil Sands Initiative and seeking to improve the biodegradation rate of naphthenic acids (NAs), a toxic by-product of the oil extraction process which can linger in the environment for decades. Two Pseudomonas strains have been found to synergistically degrade 95% of NAs. Our project focuses on engineering these Pseudomonas strains to form biofilms in the harsh tailings water environment, which can potentially increase degradation rates by two orders of magnitude, by expressing a self-associating E. coli protein.

Michigan-plate.jpg Locations of visitors to this page

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