Testing usu.html

From 2010.igem.org

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<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en" dir="ltr">
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{{:Team:Utah_State/usu_bg}}
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{{:Team:Utah_State/usu_header}}
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{{:Team:Utah_State/usu_menu}}
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<body              class="mediawiki ns-0 ltr page-Team_Utah_State">
 
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  <div id="globalWrapper">
 
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<div id="HomeCenterCenterBB">
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<!-- start content -->
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<div id="Tittle">
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<p>
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Testing
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<!a href="https://2009.igem.org/Team:Utah_State">
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<!img alt="USU iGem" src="https://static.igem.org/mediawiki/2010/5/5e/Utah_State_logo.png"/> <!/a>
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<!--- SIDEBAR LINKS--->
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<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
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<html xmlns="http://www.w3.org/1999/xhtml">
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<head>
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<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
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<title>Utah State University</title>
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</head>
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<!--------------------->
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<script type="text/javascript"><!--
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var delay=2000
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var ie4=document.all
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var curindex=0
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var totalcontent=0
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function get_total(){
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if (ie4){
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while (eval("document.all.content"+totalcontent))
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totalcontent++
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}
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else{
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while (document.getElementById("content"+totalcontent))
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totalcontent++
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}
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}
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function contract_all(){
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for (y=0;y<totalcontent;y++){
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if (ie4)
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eval("document.all.content"+y).style.display="none"
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else
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document.getElementById("content"+y).style.display="none"
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}
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}
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function expand_one(which){
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contract_all()
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if (ie4)
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eval("document.all.content"+which).style.display=""
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else
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document.getElementById("content"+which).style.display=""
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}
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function rotate_content(){
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get_total()
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contract_all()
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expand_one(curindex)
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curindex=(curindex<totalcontent-1)? curindex+1: 0
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setTimeout("rotate_content()",delay)
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}
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window.onload=rotate_content
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//--></script>
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<div class="header">
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<div class="titlebg">
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</div>
</div>
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Lorem ipsum dolor sit amet, consectetur adipiscing elit. Pellentesque vel sapien lectus, in dignissim leo. Duis diam tortor, facilisis id tincidunt in, fringilla a est. Ut elit libero, venenatis porttitor ultrices eget, rutrum sed diam. Duis semper libero posuere lorem pharetra pretium. Nulla a lacus eu ante elementum rutrum et a neque. Aenean volutpat tellus non est tempus rhoncus in nec lacus. Vivamus ac nibh at eros porta iaculis. Duis non magna ligula. Vivamus nec ipsum quis magna faucibus eleifend pretium sed lectus. Pellentesque sit amet augue non dolor tempor pharetra. Vivamus rhoncus turpis sed risus mollis sit amet ullamcorper tellus molestie. Vivamus eget lorem eget purus scelerisque sollicitudin vel ut ipsum. Nam aliquet urna odio. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Etiam semper condimentum felis, eget mollis neque pulvinar et. Cras a augue enim, a facilisis nisi. Suspendisse potenti. Sed sit amet est augue. Quisque non tortor enim, quis pretium ante.
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<div id="content0" style="display:none">
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=USU TEAM=
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<img src="https://static.igem.org/mediawiki/2010/b/b6/USU2010banner.jpg" width="965px" height="158px" border="0"></div>
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Lorem ipsum dolor sit amet, consectetur adipiscing elit. Pellentesque vel sapien lectus, in dignissim leo. Duis diam tortor, facilisis id tincidunt in, fringilla a est. Ut elit libero, venenatis porttitor ultrices eget, rutrum sed diam. Duis semper libero posuere lorem pharetra pretium. Nulla a lacus eu ante elementum rutrum et a neque. Aenean volutpat tellus non est tempus rhoncus in nec lacus. Vivamus ac nibh at eros porta iaculis. Duis non magna ligula. Vivamus nec ipsum quis magna faucibus eleifend pretium sed lectus. Pellentesque sit amet augue non dolor tempor pharetra. Vivamus rhoncus turpis sed risus mollis sit amet ullamcorper tellus molestie. Vivamus eget lorem eget purus scelerisque sollicitudin vel ut ipsum. Nam aliquet urna odio. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Etiam semper condimentum felis, eget mollis neque pulvinar et. Cras a augue enim, a facilisis nisi. Suspendisse potenti. Sed sit amet est augue. Quisque non tortor enim, quis pretium ante.
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<div id="content1" style="display:none">
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<img src="https://static.igem.org/mediawiki/2010/1/15/Image2.jpg" width="965px" height="158px" border="0"></div>
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<div id="content2" style="display:none">
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<img src="https://static.igem.org/mediawiki/2010/f/f1/Image1.jpg" width="965px" height="158px" border="0"></div>
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<div id="content3" style="display:none">
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<img src="https://static.igem.org/mediawiki/2010/b/b6/USU2010banner.jpg" width="965px" height="158px" border="0"></div>
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<div id="content4" style="display:none">
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<img src="https://static.igem.org/mediawiki/2010/d/da/Image4.jpg" width="965px" height="158px" border="0"></div>
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<div id="content5" style="display:none">
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<img src="https://static.igem.org/mediawiki/2010/5/52/Image5.jpg" width="965px" height="158px" border="0"></div>
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<div id="content6" style="display:none">
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<img src="https://static.igem.org/mediawiki/2010/b/b6/USU2010banner.jpg" width="965px" height="158px" border="0"></div>
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<div id="content7" style="display:none">
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<img src="https://static.igem.org/mediawiki/2010/0/0b/Image6.jpg" width="965px" height="158px" border="0"></div>
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<div id="content8" style="display:none">
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<img src="https://static.igem.org/mediawiki/2010/f/f6/Image7.jpg" width="965px" height="158px" border="0"></div>
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[[Image:UsuLogo_BLWboard-02.png|180px|center]]
</div>
</div>
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{{:Team:Utah_State/usu_footer}}
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<!--------- End ---->
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</head>
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<style type="text/css">
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#ana {
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width: 11em;
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  display : block;
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color : #999;
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font-weight : none;
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font-family:Century Gothic, Arial, sans-serif;
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text-decoration : none;
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background-color : #231f20;
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}
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#ana .currentPage {
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padding-left:9px;
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        border-top-style: none;
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color : #56b8df;
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width: 6em;
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display : block;
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font-weight : none;
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font-family:Century Gothic, Arial, sans-serif;
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text-decoration : none;
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}
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#ana a {
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font-size:x-small;
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text-decoration : none;
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padding-left:17px;
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color : #E0FFFF;
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font-family: Verdana, Arial, sans-serif;
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}
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#ana a:hover{
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text-decoration:underline;
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}
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#nav a {
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padding-left: 9px;
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width:10em;
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display : block;
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color : #999;
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font-weight : none;
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font-family:Century Gothic, Arial, sans-serif;
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        font size="3";
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text-decoration : none;
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        text-align : left;
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background-color : #231f20;
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}
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#nav a:hover {
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visibility:visible;
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              background-color : #474e54;
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color : #56b8df;}
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</style>
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<style>
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.ideasList{
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    margin-bottom:30px
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}
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ul#sub {
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    margin:0px;
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    padding:0px;
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    font-size:12px;
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    list-style-image:none;
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    list-style-type:none;
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}
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#sub li { margin-bottom:20px;}
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#sub li a {
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    color:#3300FF;
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    text-decoration:underline;
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}
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#sub li a:hover {
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    text-decoration:none;
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}
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table.links {
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    width:90%;
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    background-color:#000000;
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    margin-bottom:30px;
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}
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-
 
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.links tr {
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    background-color:#008E00;
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-
}
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.links td:hover {
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    background-color:#00CC00;
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}
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table.sidelinks1 {
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    width:90%;
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    background-color:#000000;
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    border-top:#262626 0.25px outset;
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    border-left:#262626 0.25px thin outset;
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    border-right:# 0.25px outset;
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-
    text-align:center;
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-
}
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-
 
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.sidelinks1 tr {
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-
    background-color: #262626;
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-
    color:#FFFFFF;
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-
}
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-
 
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.sidelinks1 td {
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-
    height:35px;
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-
}
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-
 
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.sidelinks1 td:hover {
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    background-color:#262626; <!----- Mouse over background it was #006666 -->
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}   
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table.sidelinks2 {
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-
    width:90%;
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-
    background-color:#000000;
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-
    margin-bottom:30px;
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-
    border-bottom:#12127D 0.25px outset;
+
-
    border-left:#12127D 0.25px thin outset;
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-
    border-right:#12127D 0.25px outset; -->
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    text-align:center;
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-
}
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-
 
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.sidelinks2 tr {
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-
    background-color:#009cc;
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-
    color:#FFFFFF;
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-
}
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.sidelinks2 td {
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    height:35px;
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}
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-
 
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.sidelinks2 td:hover {
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    background-color:#0000CC;
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}   
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-
 
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a.sideLinks {
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    text-decoration:none;
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    color:#FFFFFF;
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}
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a.mainLinks{
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    text-decoration:none;
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    font-size:12px;
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    color:#FFFFFF;
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}
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a.mainLinks:hover {
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}
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a.sideLinks:hover {
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}
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table.header {
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    width:100%;
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    margin-bottom:10px;
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}
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.header td {
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    width:33%;
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}   
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img
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{  border-style: none;
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}
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</style>
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<! -- END of style sheet -->
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<body bgcolor="#262626">
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<!--
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  Navigation menu
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-->
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<p><p>
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<table border="0" style="border-collapse:collapse; border-style:none;" cellspacing="0">
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  <tr>
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    <td valign="top" width="15%"><table class="sidelinks1">
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        <tr>
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          <td bgcolor="#262626"><p align="center" style="font-size:20px; color:#FFFFFF;"> <a class="sideLinks" href="https://2010.igem.org/Team:Utah_State"> <em>CyanoBricks</em></a></p></td>
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        </tr>
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        <tr>
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          <td><a class="sideLinks" href="Project_usu.html" title="choosing our project"><img src="https://static.igem.org/mediawiki/2010/2/23/Project_usu.png" height="60"></a></td>
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        </tr>
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        <tr>
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          <td><a class="sideLinks" href="Design_usu.html" title="Design"><img src="https://static.igem.org/mediawiki/2010/0/0a/Design.png" height="60"></a></td>
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        </tr>
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        <tr>
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          <td><a class="sideLinks" href="Construction_usu.html" title="Construction"><img src="https://static.igem.org/mediawiki/2010/3/37/Construction.png" height="60"></a></td>
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        </tr>
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        <!tr>
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          <!td><!a class="sideLinks" href="Modeling_usu.html" title="Software Modeling"><!img src="https://static.igem.org/mediawiki/2010/3/3c/Modeling.png" height="60"></a></td>
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        <!/tr>
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        <tr>
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          <td><a class="sideLinks" href="Testing_usu.html" title="Testing"><img src="https://static.igem.org/mediawiki/2010/7/7f/Testing.png" height="60"></a></td>
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        </tr>
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        <tr>
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          <td><a class="sideLinks" href="https://2010.igem.org/USU_protocol" title="Protocols"><img src="https://static.igem.org/mediawiki/2010/1/1d/Protocols.png" height="60"></a></td>
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        </tr>
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        <tr>
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          <td><a class="sideLinks" href="Results_usu.html" title="Results"><img src="https://static.igem.org/mediawiki/2010/1/17/Results.png" height="60"></a></td>
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        </tr>
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      </table></td>
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<!---TEXT BOXES--->
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    <td><table width=100% style="background:#CCCCCC; padding:7px; border-style:none">
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  <tr>
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    <td>
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      <font size="5" face="Century Gothic, Arial, San Serif" color =#000033>
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              <b><i>Testing</b></i></font>
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              <HR>
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<p class="class">
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Promoter Expression Testing
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<hr>
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Promoter activation was tested by constructing a protein expression device using the BioBrick promoter, the Synechocystis RBS (BBa_M11402), the GFP(mut3b) (BBa_E0040), and terminator sequences (BBa_B0015) in the vector (BBa_M11400) in E. coli. Once the device was constructed, plasmid DNA was extracted and Synechocystis was naturally transformed, using the protocol described in Zang, et al. (2007). Baseline expression readings were taken by diluting a sample of cells to the same O.D. using a spectrophotometer, and reading the GFP fluorescence with a spectrofluorometer under normal, non-stressing conditions. Stress was then induced, and fluorescence was re-measured. All fluorescence readings were converted to relative fluorescence compared to GFP expression driven by the sigA promoter under the same treatment conditions.
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    </td>
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    </tr>
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  </tr>
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<tr>
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<td>
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</td>
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</tr>
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</table>
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  </td>
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  </tr>
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</table>
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</body>
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</p><p><br />
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<head>
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<style>
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table {
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  background-color: #231f20;
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  font-color: white;
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  color:white;
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}
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a.menu {
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  background-color: #231f20;
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  color: white;
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  width: 12em;
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}
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-
 
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.firstHeading {
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-
  color:white;
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-
}
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#bodyContent {
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background-color: #231f20;
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-
}
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#content {
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background-color: #231f20;
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}
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#footer-box {
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  background-color: #231f20;
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-
}
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p {
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  color:white;
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-
}
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body {
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-
  background-color:#231f20;
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-
}
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.firstHeading {
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  display:none;
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}
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</style>
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</head>
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</p><p><br />
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</p><p>
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<style type="text/css">
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body {background-color:#231f20; }
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#content{
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border:0px;
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}
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</style>
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</p><p>
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<head>
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<style type="text/css">
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p.class{
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text-indent:1.0em;
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color: #231f20;
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font-weight : none;
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font-family:Tahoma, Arial, Sans-serif;
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font size="2.5";
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}
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p.margin
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-
{
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margin-top:0px;
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margin-bottom:0px;
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margin-right:50px;
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margin-left:50px;
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color: #231f20;
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font-weight : none;
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font-family:Tahoma, Arial, Sans-serif;
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font size="2.5";
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font-style: italic;
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}
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ul.circle {list-style-type:circle; color: #231f20;}
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</style>
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</head>
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</html>
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Latest revision as of 09:00, 26 October 2010

USU_IGEM

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Testing

Lorem ipsum dolor sit amet, consectetur adipiscing elit. Pellentesque vel sapien lectus, in dignissim leo. Duis diam tortor, facilisis id tincidunt in, fringilla a est. Ut elit libero, venenatis porttitor ultrices eget, rutrum sed diam. Duis semper libero posuere lorem pharetra pretium. Nulla a lacus eu ante elementum rutrum et a neque. Aenean volutpat tellus non est tempus rhoncus in nec lacus. Vivamus ac nibh at eros porta iaculis. Duis non magna ligula. Vivamus nec ipsum quis magna faucibus eleifend pretium sed lectus. Pellentesque sit amet augue non dolor tempor pharetra. Vivamus rhoncus turpis sed risus mollis sit amet ullamcorper tellus molestie. Vivamus eget lorem eget purus scelerisque sollicitudin vel ut ipsum. Nam aliquet urna odio. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Etiam semper condimentum felis, eget mollis neque pulvinar et. Cras a augue enim, a facilisis nisi. Suspendisse potenti. Sed sit amet est augue. Quisque non tortor enim, quis pretium ante.

USU TEAM

Lorem ipsum dolor sit amet, consectetur adipiscing elit. Pellentesque vel sapien lectus, in dignissim leo. Duis diam tortor, facilisis id tincidunt in, fringilla a est. Ut elit libero, venenatis porttitor ultrices eget, rutrum sed diam. Duis semper libero posuere lorem pharetra pretium. Nulla a lacus eu ante elementum rutrum et a neque. Aenean volutpat tellus non est tempus rhoncus in nec lacus. Vivamus ac nibh at eros porta iaculis. Duis non magna ligula. Vivamus nec ipsum quis magna faucibus eleifend pretium sed lectus. Pellentesque sit amet augue non dolor tempor pharetra. Vivamus rhoncus turpis sed risus mollis sit amet ullamcorper tellus molestie. Vivamus eget lorem eget purus scelerisque sollicitudin vel ut ipsum. Nam aliquet urna odio. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Etiam semper condimentum felis, eget mollis neque pulvinar et. Cras a augue enim, a facilisis nisi. Suspendisse potenti. Sed sit amet est augue. Quisque non tortor enim, quis pretium ante.

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