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| <br> | | <br> |
| <b>Exp 1</b> | | <b>Exp 1</b> |
| + | <center> |
| <table border=1> | | <table border=1> |
| <tr align=center> | | <tr align=center> |
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| </tr> | | </tr> |
| </table> | | </table> |
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| <br> | | <br> |
| [[Image:KAIST-exp1.png|560px]] | | [[Image:KAIST-exp1.png|560px]] |
- | <br> | + | </center> |
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- | <br>
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| <br> | | <br> |
| <br> | | <br> |
| <b>Exp 2</b> | | <b>Exp 2</b> |
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| <table border=1> | | <table border=1> |
| <tr align=center> | | <tr align=center> |
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| </tr> | | </tr> |
| </table> | | </table> |
- | <br>
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| <br> | | <br> |
| [[Image:KAIST-exp2.png|260px]] | | [[Image:KAIST-exp2.png|260px]] |
- | <br> | + | </center> |
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| <br> | | <br> |
| <br> | | <br> |
| <b>Exp 3</b> | | <b>Exp 3</b> |
| + | <center> |
| <table border=1> | | <table border=1> |
| <tr align=center> | | <tr align=center> |
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| </tr> | | </tr> |
| </table> | | </table> |
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| <br> | | <br> |
| [[Image:KAIST-exp3.png|260px]] | | [[Image:KAIST-exp3.png|260px]] |
- | <br> | + | </center> |
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| <br> | | <br> |
| <br> | | <br> |
| <b>Exp 4</b> | | <b>Exp 4</b> |
| + | <center> |
| <table border=1> | | <table border=1> |
| <tr align=center> | | <tr align=center> |
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| </tr> | | </tr> |
| </table> | | </table> |
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| <br> | | <br> |
| [[Image:KAIST-exp4.png|300px]] | | [[Image:KAIST-exp4.png|300px]] |
- | <br> | + | </center> |
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| <br> | | <br> |
| <br> | | <br> |
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| <br> | | <br> |
| <b>Exp 1</b> | | <b>Exp 1</b> |
| + | <center> |
| <table border=1> | | <table border=1> |
| <tr align=center> | | <tr align=center> |
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| </tr> | | </tr> |
| </table> | | </table> |
- | <br>
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| <br> | | <br> |
| [[Image:KAIST-exp5.png|700px]] | | [[Image:KAIST-exp5.png|700px]] |
- | <br> | + | </center> |
- | <br>
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- | <br>
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- | <br>
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| <br> | | <br> |
| <br> | | <br> |
| <b>Exp 2</b> | | <b>Exp 2</b> |
| + | <center> |
| <table border=1> | | <table border=1> |
| <tr align=center> | | <tr align=center> |
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| </tr> | | </tr> |
| </table> | | </table> |
- | <br>
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| <br> | | <br> |
| [[Image:KAIST-exp6.png|700px]] | | [[Image:KAIST-exp6.png|700px]] |
- | <br> | + | </center> |
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- | <br>
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| <br> | | <br> |
| <br> | | <br> |
| <b>Exp 3</b> | | <b>Exp 3</b> |
| + | <center> |
| <table border=1> | | <table border=1> |
| <tr align=center> | | <tr align=center> |
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| </tr> | | </tr> |
| </table> | | </table> |
- | <br>
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| <br> | | <br> |
| [[Image:KAIST-exp7.png|640px]] | | [[Image:KAIST-exp7.png|640px]] |
- | <br> | + | </center> |
- | <br>
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- | <br>
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| <br> | | <br> |
| <br> | | <br> |
| <b>Exp 4</b> | | <b>Exp 4</b> |
| + | <center> |
| <table border=1> | | <table border=1> |
| <tr align=center> | | <tr align=center> |
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| </tr> | | </tr> |
| </table> | | </table> |
- | <br>
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| <br> | | <br> |
| [[Image:KAIST-exp8.png|640px]] | | [[Image:KAIST-exp8.png|640px]] |
- | <br> | + | </center> |
- | <br>
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- | <br>
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| <br> | | <br> |
| <br> | | <br> |
| <b>Exp 5</b> | | <b>Exp 5</b> |
| + | <center> |
| <table border=1> | | <table border=1> |
| <tr align=center> | | <tr align=center> |
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| </tr> | | </tr> |
| </table> | | </table> |
- | <br>
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| <br> | | <br> |
| [[Image:KAIST-exp9.png|320px]] | | [[Image:KAIST-exp9.png|320px]] |
- | <br> | + | </center> |
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- | <br>
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| <br> | | <br> |
| <br> | | <br> |
| <b>Exp 6</b> | | <b>Exp 6</b> |
| + | <center> |
| <table border=1> | | <table border=1> |
| <tr align=center> | | <tr align=center> |
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| </tr> | | </tr> |
| </table> | | </table> |
- | <br>
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| <br> | | <br> |
| [[Image:KAIST-exp10.png|320px]] | | [[Image:KAIST-exp10.png|320px]] |
- | <br> | + | </center> |
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- | <br>
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- | <br>
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| <br> | | <br> |
| <br> | | <br> |
| <b>Exp 7</b> | | <b>Exp 7</b> |
- | <table border=1> | + | <center> |
- | <tr align=center>
| + | |
- | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | <table border=1>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">a</td><td width = "255px">3'UTR</td><td width = "200px">62*C</td><td width = "223px">-</td><td width = "70px">-</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">b</td><td width = "255px">5'UTR</td><td width = "200px">62*C</td><td width = "223px">-</td><td width = "70px">-</td></tr>
| + | |
- | </table>
| + | |
- | <br>
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- | <br>
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- | <br>
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- | <b>Exp 8</b>
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| <table border=1> | | <table border=1> |
| <tr align=center> | | <tr align=center> |
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Line 459: |
| </tr> | | </tr> |
| </table> | | </table> |
- | <br>
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| <br> | | <br> |
| [[Image:KAIST-exp12.png|400px]] | | [[Image:KAIST-exp12.png|400px]] |
| + | </center> |
| <br> | | <br> |
| <br> | | <br> |
- | <br>
| + | <b>Exp 8</b> |
- | <br>
| + | <center> |
- | <br>
| + | |
- | <br>
| + | |
- | <b>Exp 9</b> | + | |
| <table border=1> | | <table border=1> |
| <tr align=center> | | <tr align=center> |
Line 536: |
Line 488: |
| </tr> | | </tr> |
| </table> | | </table> |
- | <br>
| |
| <br> | | <br> |
| [[Image:KAIST-exp13.png|400px]] | | [[Image:KAIST-exp13.png|400px]] |
| + | </center> |
| <br> | | <br> |
| <br> | | <br> |
- | <br>
| + | <b>Exp 9</b> |
- | <br>
| + | <center> |
- | <br>
| + | |
- | <br>
| + | |
- | <b>Exp 10</b> | + | |
| <table border=1> | | <table border=1> |
| <tr align=center> | | <tr align=center> |
Line 562: |
Line 511: |
| </tr> | | </tr> |
| </table> | | </table> |
- | <br>
| |
| <br> | | <br> |
| [[Image:KAIST-exp14.png|320px]] | | [[Image:KAIST-exp14.png|320px]] |
- | <br> | + | </center> |
- | <br>
| + | |
- | <br>
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- | <br>
| + | |
| <br> | | <br> |
| <br> | | <br> |
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Line 545: |
| <br> | | <br> |
| <b>Exp 1</b> | | <b>Exp 1</b> |
| + | <center> |
| <table border=1> | | <table border=1> |
| <tr align=center> | | <tr align=center> |
Line 642: |
Line 588: |
| <td width = "40px">l</td><td width = "255px"><font color=red>V_STAT1</font></td><td width = "200px">60*C</td><td width = "223px">100%</td><td width = "70px">2151bp</td> | | <td width = "40px">l</td><td width = "255px"><font color=red>V_STAT1</font></td><td width = "200px">60*C</td><td width = "223px">100%</td><td width = "70px">2151bp</td> |
| </tr> | | </tr> |
- |
| |
| </table> | | </table> |
- | <br>
| |
| <br> | | <br> |
| [[Image:KAIST-exp21.png|700px]] | | [[Image:KAIST-exp21.png|700px]] |
- | <br> | + | </center> |
- | <br>
| + | |
- | <br>
| + | |
| <br> | | <br> |
| <br> | | <br> |
| <b>Exp 2</b> | | <b>Exp 2</b> |
| + | <center> |
| <table border=1> | | <table border=1> |
| <tr align=center> | | <tr align=center> |
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| <td width = "40px">l</td><td width = "255px"><font color=red>VB_FGFR</font></td><td width = "200px">60*C</td><td width = "223px">100%</td><td width = "70px">1420bp</td> | | <td width = "40px">l</td><td width = "255px"><font color=red>VB_FGFR</font></td><td width = "200px">60*C</td><td width = "223px">100%</td><td width = "70px">1420bp</td> |
| </tr> | | </tr> |
- |
| |
| </table> | | </table> |
- | <br>
| |
| <br> | | <br> |
| [[Image:KAIST-exp22.png|700px]] | | [[Image:KAIST-exp22.png|700px]] |
- | <br> | + | </center> |
- | <br>
| + | |
- | <br>
| + | |
| <br> | | <br> |
| <br> | | <br> |
| <b>Exp 3</b> | | <b>Exp 3</b> |
| + | <center> |
| <table border=1> | | <table border=1> |
| <tr align=center> | | <tr align=center> |
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Line 701: |
| </tr> | | </tr> |
| </table> | | </table> |
- | <br>
| |
| <br> | | <br> |
| [[Image:KAIST-exp23.png|700px]] | | [[Image:KAIST-exp23.png|700px]] |
- | <br> | + | </center> |
- | <br>
| + | |
- | <br>
| + | |
| <br> | | <br> |
| <br> | | <br> |
| <b>Exp 4</b> | | <b>Exp 4</b> |
| + | <center> |
| <table border=1> | | <table border=1> |
| <tr align=center> | | <tr align=center> |
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| | | |
| </table> | | </table> |
- | <br>
| |
| <br> | | <br> |
| [[Image:KAIST-exp24.png|360px]] | | [[Image:KAIST-exp24.png|360px]] |
- | <br> | + | </center> |
- | <br>
| + | |
- | <br>
| + | |
| <br> | | <br> |
| <br> | | <br> |
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| <br> | | <br> |
| <b>Exp 1</b> | | <b>Exp 1</b> |
| + | <center> |
| <table border=1> | | <table border=1> |
| <tr align=center> | | <tr align=center> |
Line 838: |
Line 774: |
| </tr> | | </tr> |
| </table> | | </table> |
- |
| |
| <table border=1> | | <table border=1> |
| <tr align=center> | | <tr align=center> |
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Line 800: |
| </tr> | | </tr> |
| <tr align=center> | | <tr align=center> |
- | <td width = "40px">i</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">30%</td><td width = "70px">1107bp</td> | + | <td width = "40px">i</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">50*C</td><td width = "223px">50%</td><td width = "70px">1107bp</td> |
| </tr> | | </tr> |
| <tr align=center> | | <tr align=center> |
- | <td width = "40px">j</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">50%</td><td width = "70px">1107bp</td> | + | <td width = "40px">j</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">52*C</td><td width = "223px">80%</td><td width = "70px">1107bp</td> |
| </tr> | | </tr> |
| <tr align=center> | | <tr align=center> |
- | <td width = "40px">k</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>4 | + | <td width = "40px">k</td><td width = "255px"><font color=red>B_Fusion antibody receptor</font></td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td> |
| </tr> | | </tr> |
| <tr align=center> | | <tr align=center> |
- | <td width = "40px">l</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td> | + | <td width = "40px">l</td><td width = "255px"><font color=red>B_Fusion antibody receptor</font></td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td> |
| </tr> | | </tr> |
| </table> | | </table> |
- | <br>
| |
| <br> | | <br> |
| [[Image:KAIST-exp25.png|360px]] | | [[Image:KAIST-exp25.png|360px]] |
| + | </center> |
| <br> | | <br> |
| <br> | | <br> |
| <b>Exp 2</b> | | <b>Exp 2</b> |
| + | <center> |
| <table border=1> | | <table border=1> |
| <tr align=center> | | <tr align=center> |
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| <table border=1> | | <table border=1> |
| <tr align=center> | | <tr align=center> |
- | <td width = "40px">a</td><td width = "255px">HA1</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | + | <td width = "40px">a</td><td width = "255px"><font color=red>HA1</font></td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> |
| </tr> | | </tr> |
| <tr align=center> | | <tr align=center> |
- | <td width = "40px">b</td><td width = "255px">HA1</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | + | <td width = "40px">b</td><td width = "255px"><font color=red>HA1</font></td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> |
| </tr> | | </tr> |
| <tr align=center> | | <tr align=center> |
- | <td width = "40px">c</td><td width = "255px">HA1</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | + | <td width = "40px">c</td><td width = "255px"><font color=red>HA1</font></td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> |
| </tr> | | </tr> |
| <tr align=center> | | <tr align=center> |
- | <td width = "40px">d</td><td width = "255px">HA1</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | + | <td width = "40px">d</td><td width = "255px"><font color=red>HA1</font></td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> |
| </tr> | | </tr> |
| <tr align=center> | | <tr align=center> |
- | <td width = "40px">e</td><td width = "255px">HA2</td><td width = "200px">50*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | + | <td width = "40px">e</td><td width = "255px"><font color=red>HA2</font></td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> |
| </tr> | | </tr> |
| <tr align=center> | | <tr align=center> |
- | <td width = "40px">f</td><td width = "255px">HA2</td><td width = "200px">52*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | + | <td width = "40px">f</td><td width = "255px"><font color=red>HA2</font></td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> |
| </tr> | | </tr> |
| <tr align=center> | | <tr align=center> |
- | <td width = "40px">g</td><td width = "255px">HA2</td><td width = "200px">54*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | + | <td width = "40px">g</td><td width = "255px"><font color=red>HA2</font></td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> |
| </tr> | | </tr> |
| <tr align=center> | | <tr align=center> |
- | <td width = "40px">h</td><td width = "255px">HA2</td><td width = "200px">56*C</td><td width = "223px">80%</td><td width = "70px">87bp</td> | + | <td width = "40px">h</td><td width = "255px"><font color=red>HA2</font></td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> |
| </tr> | | </tr> |
| <tr align=center> | | <tr align=center> |
- | <td width = "40px">i</td><td width = "255px">Proximal promoter</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | + | <td width = "40px">i</td><td width = "255px"><font color=red>Proximal promoter</font></td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> |
| </tr> | | </tr> |
| <tr align=center> | | <tr align=center> |
- | <td width = "40px">j</td><td width = "255px">Proximal promoter</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | + | <td width = "40px">j</td><td width = "255px"><font color=red>Proximal promoter</font></td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> |
| </tr> | | </tr> |
| <tr align=center> | | <tr align=center> |
- | <td width = "40px">k</td><td width = "255px">Proximal promoter</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | + | <td width = "40px">k</td><td width = "255px"><font color=red>Proximal promoter</font></td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> |
| </tr> | | </tr> |
| <tr align=center> | | <tr align=center> |
- | <td width = "40px">l</td><td width = "255px">Proximal promoter</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> | + | <td width = "40px">l</td><td width = "255px"><font color=red>Proximal promoter</font></td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">120bp</td> |
| </tr> | | </tr> |
| <tr align=center> | | <tr align=center> |
- | <td width = "40px">m</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td> | + | <td width = "40px">m</td><td width = "255px"><font color=red>5' UTR</font></td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td> |
| </tr> | | </tr> |
| <tr align=center> | | <tr align=center> |
- | <td width = "40px">n</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td> | + | <td width = "40px">n</td><td width = "255px"><font color=red>5' UTR</font></td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td> |
| </tr> | | </tr> |
| <tr align=center> | | <tr align=center> |
- | <td width = "40px">o</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td> | + | <td width = "40px">o</td><td width = "255px"><font color=red>3' UTR</font></td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td> |
| </tr> | | </tr> |
| <tr align=center> | | <tr align=center> |
- | <td width = "40px">p</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td> | + | <td width = "40px">p</td><td width = "255px"><font color=red>3' UTR</font></td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td> |
| </tr> | | </tr> |
| </table> | | </table> |
- | <br>
| |
| <br> | | <br> |
| [[Image:KAIST-exp26.png|360px]] | | [[Image:KAIST-exp26.png|360px]] |
| + | </center> |
| <br> | | <br> |
| <br> | | <br> |
Line 972: |
Line 908: |
| <br> | | <br> |
| <b>Exp 1</b> | | <b>Exp 1</b> |
| + | <center> |
| <table border=1> | | <table border=1> |
| <tr align=center> | | <tr align=center> |
Line 977: |
Line 914: |
| </tr> | | </tr> |
| </table> | | </table> |
- |
| |
| <table border=1> | | <table border=1> |
| <tr align=center> | | <tr align=center> |
- | <td width = "40px">a</td><td width = "255px">Selection marker(41)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td> | + | <td width = "40px">a</td><td width = "255px"><font color=red>HA2+3'UTR</font></td><td width = "200px">58*C</td><td width = "223px">100%</td><td width = "70px">531bp</td> |
| </tr> | | </tr> |
| <tr align=center> | | <tr align=center> |
- | <td width = "40px">b</td><td width = "255px">Selection marker(41)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td> | + | <td width = "40px">b</td><td width = "255px"><font color=red>F_Fusion antibody receptor</font></td><td width = "200px">58*C</td><td width = "223px">100%</td><td width = "70px">1107bp</td> |
| </tr> | | </tr> |
| <tr align=center> | | <tr align=center> |
- | <td width = "40px">c</td><td width = "255px">Selection marker(41)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td> | + | <td width = "40px">c</td><td width = "255px"><font color=red>F_Fusion antibody receptor</font></td><td width = "200px">58*C</td><td width = "223px">100%</td><td width = "70px">1107bp</td> |
| </tr> | | </tr> |
| <tr align=center> | | <tr align=center> |
- | <td width = "40px">d</td><td width = "255px">Selection marker(41)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td> | + | <td width = "40px">d</td><td width = "255px"><font color=red>B_Fusion antibody receptor</font></td><td width = "200px">58*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td> |
| </tr> | | </tr> |
| <tr align=center> | | <tr align=center> |
- | <td width = "40px">e</td><td width = "255px">Selection marker(42)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td> | + | <td width = "40px">e</td><td width = "255px"><font color=red>B_Fusion antibody receptor</font></td><td width = "200px">58*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td> |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">f</td><td width = "255px">Selection marker(42)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">g</td><td width = "255px">Selection marker(42)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">h</td><td width = "255px">Selection marker(42)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">i</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">30%</td><td width = "70px">1107bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">j</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">50%</td><td width = "70px">1107bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">k</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>4
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">l</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>
| + | |
| </tr> | | </tr> |
| </table> | | </table> |
| <br> | | <br> |
- | <br>
| + | [[Image:KAIST-exp27.png|360px]] |
- | [[Image:KAIST-exp25.png|360px]] | + | </center> |
- | <br>
| + | |
- | <br>
| + | |
- | <b>Exp 2</b>
| + | |
- | <table border=1>
| + | |
- | <tr align=center>
| + | |
- | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | <table border=1>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">a</td><td width = "255px">HA1</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">b</td><td width = "255px">HA1</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">c</td><td width = "255px">HA1</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">d</td><td width = "255px">HA1</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">e</td><td width = "255px">HA2</td><td width = "200px">50*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">f</td><td width = "255px">HA2</td><td width = "200px">52*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">g</td><td width = "255px">HA2</td><td width = "200px">54*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">h</td><td width = "255px">HA2</td><td width = "200px">56*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">i</td><td width = "255px">Proximal promoter</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">j</td><td width = "255px">Proximal promoter</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">k</td><td width = "255px">Proximal promoter</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">l</td><td width = "255px">Proximal promoter</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">m</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">n</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">o</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">p</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | [[Image:KAIST-exp26.png|360px]]
| + | |
| <br> | | <br> |
| <br> | | <br> |
Line 1,111: |
Line 965: |
| <br> | | <br> |
| <b>Exp 1</b> | | <b>Exp 1</b> |
| + | <center> |
| <table border=1> | | <table border=1> |
| <tr align=center> | | <tr align=center> |
Line 1,116: |
Line 971: |
| </tr> | | </tr> |
| </table> | | </table> |
- |
| |
| <table border=1> | | <table border=1> |
| <tr align=center> | | <tr align=center> |
- | <td width = "40px">a</td><td width = "255px">Selection marker(41)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td> | + | <td width = "40px">a</td><td width = "255px">Fusion antibody receptor</td><td width = "200px">58*C</td><td width = "223px">50%</td><td width = "70px">2493bp</td> |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">b</td><td width = "255px">Selection marker(41)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">c</td><td width = "255px">Selection marker(41)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">d</td><td width = "255px">Selection marker(41)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">e</td><td width = "255px">Selection marker(42)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">f</td><td width = "255px">Selection marker(42)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">g</td><td width = "255px">Selection marker(42)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">h</td><td width = "255px">Selection marker(42)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">i</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">30%</td><td width = "70px">1107bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">j</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">50%</td><td width = "70px">1107bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">k</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>4
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">l</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>
| + | |
| </tr> | | </tr> |
| </table> | | </table> |
| <br> | | <br> |
- | <br>
| + | [[Image:KAIST-exp28.png|360px]] |
- | [[Image:KAIST-exp25.png|360px]] | + | </center> |
- | <br>
| + | |
- | <br>
| + | |
- | <b>Exp 2</b>
| + | |
- | <table border=1>
| + | |
- | <tr align=center>
| + | |
- | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | <table border=1>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">a</td><td width = "255px">HA1</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">b</td><td width = "255px">HA1</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">c</td><td width = "255px">HA1</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">d</td><td width = "255px">HA1</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">e</td><td width = "255px">HA2</td><td width = "200px">50*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">f</td><td width = "255px">HA2</td><td width = "200px">52*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">g</td><td width = "255px">HA2</td><td width = "200px">54*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">h</td><td width = "255px">HA2</td><td width = "200px">56*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">i</td><td width = "255px">Proximal promoter</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">j</td><td width = "255px">Proximal promoter</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">k</td><td width = "255px">Proximal promoter</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">l</td><td width = "255px">Proximal promoter</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">m</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">n</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">o</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">p</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | [[Image:KAIST-exp26.png|360px]]
| + | |
| <br> | | <br> |
| <br> | | <br> |
| | | |
- | == 9/17 PCR result == | + | == 9/21 PCR result == |
| | | |
| <span style=font-size:12px><b>PCR composition </b></span> | | <span style=font-size:12px><b>PCR composition </b></span> |
Line 1,250: |
Line 1,010: |
| <br> | | <br> |
| <b>Exp 1</b> | | <b>Exp 1</b> |
| + | <center> |
| <table border=1> | | <table border=1> |
| <tr align=center> | | <tr align=center> |
Line 1,255: |
Line 1,016: |
| </tr> | | </tr> |
| </table> | | </table> |
- |
| |
| <table border=1> | | <table border=1> |
| <tr align=center> | | <tr align=center> |
- | <td width = "40px">a</td><td width = "255px">Selection marker(41)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td> | + | <td width = "40px">a</td><td width = "255px"><font color=red>F_Fusion antibody receptor</font></td><td width = "200px">58*C</td><td width = "223px">100%</td><td width = "70px">1107bp</td> |
| </tr> | | </tr> |
| <tr align=center> | | <tr align=center> |
- | <td width = "40px">b</td><td width = "255px">Selection marker(41)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td> | + | <td width = "40px">b</td><td width = "255px"><font color=red>B_Fusion antibody receptor</font></td><td width = "200px">58*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td> |
| </tr> | | </tr> |
| <tr align=center> | | <tr align=center> |
- | <td width = "40px">c</td><td width = "255px">Selection marker(41)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td> | + | <td width = "40px">c</td><td width = "255px">Core promoter</td><td width = "200px">58*C</td><td width = "223px">0%</td><td width = "70px">--</td> |
| </tr> | | </tr> |
| <tr align=center> | | <tr align=center> |
- | <td width = "40px">d</td><td width = "255px">Selection marker(41)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td> | + | <td width = "40px">d</td><td width = "255px">Selection marker(42)</td><td width = "200px">58*C</td><td width = "223px">0%</td><td width = "70px">--</td> |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">e</td><td width = "255px">Selection marker(42)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">f</td><td width = "255px">Selection marker(42)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">g</td><td width = "255px">Selection marker(42)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">h</td><td width = "255px">Selection marker(42)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">i</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">30%</td><td width = "70px">1107bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">j</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">50%</td><td width = "70px">1107bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">k</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>4
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">l</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>
| + | |
| </tr> | | </tr> |
| </table> | | </table> |
| <br> | | <br> |
- | <br>
| + | [[Image:KAIST-exp29.png|360px]] |
- | [[Image:KAIST-exp25.png|360px]] | + | </center> |
| <br> | | <br> |
| <br> | | <br> |
| <b>Exp 2</b> | | <b>Exp 2</b> |
| + | <center> |
| <table border=1> | | <table border=1> |
| <tr align=center> | | <tr align=center> |
Line 1,307: |
Line 1,044: |
| <table border=1> | | <table border=1> |
| <tr align=center> | | <tr align=center> |
- | <td width = "40px">a</td><td width = "255px">HA1</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | + | <td width = "40px">a</td><td width = "255px">Selection marker(41)</td><td width = "200px">58*C</td><td width = "223px">0%</td><td width = "70px">--</td> |
| </tr> | | </tr> |
| <tr align=center> | | <tr align=center> |
- | <td width = "40px">b</td><td width = "255px">HA1</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">87bp</td> | + | <td width = "40px">b</td><td width = "255px">Selection marker(42)</td><td width = "200px">58*C</td><td width = "223px">0%</td><td width = "70px">--</td> |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">c</td><td width = "255px">HA1</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">d</td><td width = "255px">HA1</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">e</td><td width = "255px">HA2</td><td width = "200px">50*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">f</td><td width = "255px">HA2</td><td width = "200px">52*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">g</td><td width = "255px">HA2</td><td width = "200px">54*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">h</td><td width = "255px">HA2</td><td width = "200px">56*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">i</td><td width = "255px">Proximal promoter</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">j</td><td width = "255px">Proximal promoter</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">k</td><td width = "255px">Proximal promoter</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">l</td><td width = "255px">Proximal promoter</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">m</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">n</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">o</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">p</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td>
| + | |
| </tr> | | </tr> |
| </table> | | </table> |
| <br> | | <br> |
- | <br>
| + | [[Image:KAIST-exp30.png|360px]] |
- | [[Image:KAIST-exp26.png|360px]] | + | </center> |
| <br> | | <br> |
| <br> | | <br> |
| | | |
- | | + | == 10/5 PCR result == |
- | == 9/17 PCR result == | + | |
| | | |
| <span style=font-size:12px><b>PCR composition </b></span> | | <span style=font-size:12px><b>PCR composition </b></span> |
Line 1,390: |
Line 1,084: |
| <br> | | <br> |
| <b>Exp 1</b> | | <b>Exp 1</b> |
| + | <center> |
| <table border=1> | | <table border=1> |
| <tr align=center> | | <tr align=center> |
Line 1,395: |
Line 1,090: |
| </tr> | | </tr> |
| </table> | | </table> |
- |
| |
| <table border=1> | | <table border=1> |
| <tr align=center> | | <tr align=center> |
- | <td width = "40px">a</td><td width = "255px">Selection marker(41)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td> | + | <td width = "40px">a</td><td width = "255px"><font color=blue>Ig-like</font></td><td width = "200px">58*C</td><td width = "223px">100%</td><td width = "70px">339bp</td> |
| </tr> | | </tr> |
| <tr align=center> | | <tr align=center> |
- | <td width = "40px">b</td><td width = "255px">Selection marker(41)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td> | + | <td width = "40px">b</td><td width = "255px"><font color=blue>Sig + Ig-like</font></td><td width = "200px">58*C</td><td width = "223px">100%</td><td width = "70px">426bp</td> |
| </tr> | | </tr> |
| <tr align=center> | | <tr align=center> |
- | <td width = "40px">c</td><td width = "255px">Selection marker(41)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td> | + | <td width = "40px">c</td><td width = "255px"><font color=blue>Antibody</font></td><td width = "200px">58*C</td><td width = "223px">90%</td><td width = "70px">669bp</td> |
| </tr> | | </tr> |
| <tr align=center> | | <tr align=center> |
- | <td width = "40px">d</td><td width = "255px">Selection marker(41)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td> | + | <td width = "40px">d</td><td width = "255px"><font color=blue>F_FGFR</font></td><td width = "200px">58*C</td><td width = "223px">50%</td><td width = "70px">1095bp</td> |
| </tr> | | </tr> |
| <tr align=center> | | <tr align=center> |
- | <td width = "40px">e</td><td width = "255px">Selection marker(42)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td> | + | <td width = "40px">e</td><td width = "255px"><font color=blue>B_FGFR</font></td><td width = "200px">58*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td> |
| </tr> | | </tr> |
| <tr align=center> | | <tr align=center> |
- | <td width = "40px">f</td><td width = "255px">Selection marker(42)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td> | + | <td width = "40px">f</td><td width = "255px"><font color=blue>STAT1</font></td><td width = "200px">58*C</td><td width = "223px">100%</td><td width = "70px">2139bp</td> |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">g</td><td width = "255px">Selection marker(42)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">h</td><td width = "255px">Selection marker(42)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">i</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">30%</td><td width = "70px">1107bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">j</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">50%</td><td width = "70px">1107bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">k</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>4
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">l</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>
| + | |
| </tr> | | </tr> |
| </table> | | </table> |
- | <br>
| + | [[Image:KAIST-exp31.png|360px]] |
- | <br>
| + | </center> |
- | [[Image:KAIST-exp25.png|360px]] | + | |
- | <br>
| + | |
- | <br>
| + | |
- | <b>Exp 2</b>
| + | |
- | <table border=1>
| + | |
- | <tr align=center>
| + | |
- | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | <table border=1>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">a</td><td width = "255px">HA1</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">b</td><td width = "255px">HA1</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">c</td><td width = "255px">HA1</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">d</td><td width = "255px">HA1</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">e</td><td width = "255px">HA2</td><td width = "200px">50*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">f</td><td width = "255px">HA2</td><td width = "200px">52*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">g</td><td width = "255px">HA2</td><td width = "200px">54*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">h</td><td width = "255px">HA2</td><td width = "200px">56*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">i</td><td width = "255px">Proximal promoter</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">j</td><td width = "255px">Proximal promoter</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">k</td><td width = "255px">Proximal promoter</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">l</td><td width = "255px">Proximal promoter</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">m</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">n</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">o</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">p</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | [[Image:KAIST-exp26.png|360px]]
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | | + | |
- | | + | |
- | == 9/17 PCR result ==
| + | |
- | | + | |
- | <span style=font-size:12px><b>PCR composition </b></span>
| + | |
- | <table border=1>
| + | |
- | <tr align=center>
| + | |
- | <td><b>Materials</b></td><td><b>Amount</b></td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td>Template</td><td>100pmole</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td>Primer A</td><td>50pmole</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td>Primer B</td><td>50pmole</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td>Bioneer Accupower HF PCR premix </td><td> < 1ul</td>
| + | |
- | </tr>
| + | |
- | <tr align=center><td>DW (3rd sterile water)</td><td>rest</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td>Total</td><td>50ul</td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | <b>Exp 1</b>
| + | |
- | <table border=1>
| + | |
- | <tr align=center>
| + | |
- | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | | + | |
- | <table border=1>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">a</td><td width = "255px">Selection marker(41)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">b</td><td width = "255px">Selection marker(41)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">c</td><td width = "255px">Selection marker(41)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">d</td><td width = "255px">Selection marker(41)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">e</td><td width = "255px">Selection marker(42)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">f</td><td width = "255px">Selection marker(42)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">g</td><td width = "255px">Selection marker(42)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">h</td><td width = "255px">Selection marker(42)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">i</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">30%</td><td width = "70px">1107bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">j</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">50%</td><td width = "70px">1107bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">k</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>4
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">l</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | [[Image:KAIST-exp25.png|360px]]
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | <b>Exp 2</b>
| + | |
- | <table border=1>
| + | |
- | <tr align=center>
| + | |
- | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | <table border=1>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">a</td><td width = "255px">HA1</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">b</td><td width = "255px">HA1</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">c</td><td width = "255px">HA1</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">d</td><td width = "255px">HA1</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">e</td><td width = "255px">HA2</td><td width = "200px">50*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">f</td><td width = "255px">HA2</td><td width = "200px">52*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">g</td><td width = "255px">HA2</td><td width = "200px">54*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">h</td><td width = "255px">HA2</td><td width = "200px">56*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">i</td><td width = "255px">Proximal promoter</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">j</td><td width = "255px">Proximal promoter</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">k</td><td width = "255px">Proximal promoter</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">l</td><td width = "255px">Proximal promoter</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">m</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">n</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">o</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">p</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | [[Image:KAIST-exp26.png|360px]]
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | | + | |
- | | + | |
- | == 9/17 PCR result ==
| + | |
- | | + | |
- | <span style=font-size:12px><b>PCR composition </b></span>
| + | |
- | <table border=1>
| + | |
- | <tr align=center>
| + | |
- | <td><b>Materials</b></td><td><b>Amount</b></td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td>Template</td><td>100pmole</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td>Primer A</td><td>50pmole</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td>Primer B</td><td>50pmole</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td>Bioneer Accupower HF PCR premix </td><td> < 1ul</td>
| + | |
- | </tr>
| + | |
- | <tr align=center><td>DW (3rd sterile water)</td><td>rest</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td>Total</td><td>50ul</td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | <b>Exp 1</b>
| + | |
- | <table border=1>
| + | |
- | <tr align=center>
| + | |
- | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | | + | |
- | <table border=1>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">a</td><td width = "255px">Selection marker(41)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">b</td><td width = "255px">Selection marker(41)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">c</td><td width = "255px">Selection marker(41)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">d</td><td width = "255px">Selection marker(41)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">e</td><td width = "255px">Selection marker(42)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">f</td><td width = "255px">Selection marker(42)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">g</td><td width = "255px">Selection marker(42)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">h</td><td width = "255px">Selection marker(42)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">i</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">30%</td><td width = "70px">1107bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">j</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">50%</td><td width = "70px">1107bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">k</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>4
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">l</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | [[Image:KAIST-exp25.png|360px]]
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | <b>Exp 2</b>
| + | |
- | <table border=1>
| + | |
- | <tr align=center>
| + | |
- | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | <table border=1>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">a</td><td width = "255px">HA1</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">b</td><td width = "255px">HA1</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">c</td><td width = "255px">HA1</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">d</td><td width = "255px">HA1</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">e</td><td width = "255px">HA2</td><td width = "200px">50*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">f</td><td width = "255px">HA2</td><td width = "200px">52*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">g</td><td width = "255px">HA2</td><td width = "200px">54*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">h</td><td width = "255px">HA2</td><td width = "200px">56*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">i</td><td width = "255px">Proximal promoter</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">j</td><td width = "255px">Proximal promoter</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">k</td><td width = "255px">Proximal promoter</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">l</td><td width = "255px">Proximal promoter</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">m</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">n</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">o</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">p</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | [[Image:KAIST-exp26.png|360px]]
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | | + | |
- | | + | |
- | == 9/17 PCR result ==
| + | |
- | | + | |
- | <span style=font-size:12px><b>PCR composition </b></span>
| + | |
- | <table border=1>
| + | |
- | <tr align=center>
| + | |
- | <td><b>Materials</b></td><td><b>Amount</b></td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td>Template</td><td>100pmole</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td>Primer A</td><td>50pmole</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td>Primer B</td><td>50pmole</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td>Bioneer Accupower HF PCR premix </td><td> < 1ul</td>
| + | |
- | </tr>
| + | |
- | <tr align=center><td>DW (3rd sterile water)</td><td>rest</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td>Total</td><td>50ul</td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | <b>Exp 1</b>
| + | |
- | <table border=1>
| + | |
- | <tr align=center>
| + | |
- | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | | + | |
- | <table border=1>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">a</td><td width = "255px">Selection marker(41)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">b</td><td width = "255px">Selection marker(41)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">c</td><td width = "255px">Selection marker(41)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">d</td><td width = "255px">Selection marker(41)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">e</td><td width = "255px">Selection marker(42)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">f</td><td width = "255px">Selection marker(42)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">g</td><td width = "255px">Selection marker(42)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">h</td><td width = "255px">Selection marker(42)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">i</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">30%</td><td width = "70px">1107bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">j</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">50%</td><td width = "70px">1107bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">k</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>4
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">l</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | [[Image:KAIST-exp25.png|360px]]
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | <b>Exp 2</b>
| + | |
- | <table border=1>
| + | |
- | <tr align=center>
| + | |
- | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | <table border=1>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">a</td><td width = "255px">HA1</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">b</td><td width = "255px">HA1</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">c</td><td width = "255px">HA1</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">d</td><td width = "255px">HA1</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">e</td><td width = "255px">HA2</td><td width = "200px">50*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">f</td><td width = "255px">HA2</td><td width = "200px">52*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">g</td><td width = "255px">HA2</td><td width = "200px">54*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">h</td><td width = "255px">HA2</td><td width = "200px">56*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">i</td><td width = "255px">Proximal promoter</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">j</td><td width = "255px">Proximal promoter</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">k</td><td width = "255px">Proximal promoter</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">l</td><td width = "255px">Proximal promoter</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">m</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">n</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">o</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">p</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | [[Image:KAIST-exp26.png|360px]]
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | | + | |
- | | + | |
- | == 9/17 PCR result ==
| + | |
- | | + | |
- | <span style=font-size:12px><b>PCR composition </b></span>
| + | |
- | <table border=1>
| + | |
- | <tr align=center>
| + | |
- | <td><b>Materials</b></td><td><b>Amount</b></td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td>Template</td><td>100pmole</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td>Primer A</td><td>50pmole</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td>Primer B</td><td>50pmole</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td>Bioneer Accupower HF PCR premix </td><td> < 1ul</td>
| + | |
- | </tr>
| + | |
- | <tr align=center><td>DW (3rd sterile water)</td><td>rest</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td>Total</td><td>50ul</td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | <b>Exp 1</b>
| + | |
- | <table border=1>
| + | |
- | <tr align=center>
| + | |
- | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | | + | |
- | <table border=1>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">a</td><td width = "255px">Selection marker(41)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">b</td><td width = "255px">Selection marker(41)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">c</td><td width = "255px">Selection marker(41)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">d</td><td width = "255px">Selection marker(41)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">e</td><td width = "255px">Selection marker(42)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">f</td><td width = "255px">Selection marker(42)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">g</td><td width = "255px">Selection marker(42)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">h</td><td width = "255px">Selection marker(42)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">i</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">30%</td><td width = "70px">1107bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">j</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">50%</td><td width = "70px">1107bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">k</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>4
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">l</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | [[Image:KAIST-exp25.png|360px]]
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | <b>Exp 2</b>
| + | |
- | <table border=1>
| + | |
- | <tr align=center>
| + | |
- | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | <table border=1>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">a</td><td width = "255px">HA1</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">b</td><td width = "255px">HA1</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">c</td><td width = "255px">HA1</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">d</td><td width = "255px">HA1</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">e</td><td width = "255px">HA2</td><td width = "200px">50*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">f</td><td width = "255px">HA2</td><td width = "200px">52*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">g</td><td width = "255px">HA2</td><td width = "200px">54*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">h</td><td width = "255px">HA2</td><td width = "200px">56*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">i</td><td width = "255px">Proximal promoter</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">j</td><td width = "255px">Proximal promoter</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">k</td><td width = "255px">Proximal promoter</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">l</td><td width = "255px">Proximal promoter</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">m</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">n</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">o</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">p</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | [[Image:KAIST-exp26.png|360px]]
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | | + | |
- | | + | |
- | == 9/17 PCR result ==
| + | |
- | | + | |
- | <span style=font-size:12px><b>PCR composition </b></span>
| + | |
- | <table border=1>
| + | |
- | <tr align=center>
| + | |
- | <td><b>Materials</b></td><td><b>Amount</b></td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td>Template</td><td>100pmole</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td>Primer A</td><td>50pmole</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td>Primer B</td><td>50pmole</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td>Bioneer Accupower HF PCR premix </td><td> < 1ul</td>
| + | |
- | </tr>
| + | |
- | <tr align=center><td>DW (3rd sterile water)</td><td>rest</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td>Total</td><td>50ul</td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | <b>Exp 1</b>
| + | |
- | <table border=1>
| + | |
- | <tr align=center>
| + | |
- | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | | + | |
- | <table border=1>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">a</td><td width = "255px">Selection marker(41)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">b</td><td width = "255px">Selection marker(41)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">c</td><td width = "255px">Selection marker(41)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">d</td><td width = "255px">Selection marker(41)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">e</td><td width = "255px">Selection marker(42)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">f</td><td width = "255px">Selection marker(42)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">g</td><td width = "255px">Selection marker(42)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">h</td><td width = "255px">Selection marker(42)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">i</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">30%</td><td width = "70px">1107bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">j</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">50%</td><td width = "70px">1107bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">k</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>4
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">l</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | [[Image:KAIST-exp25.png|360px]]
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | <b>Exp 2</b>
| + | |
- | <table border=1>
| + | |
- | <tr align=center>
| + | |
- | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | <table border=1>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">a</td><td width = "255px">HA1</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">b</td><td width = "255px">HA1</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">c</td><td width = "255px">HA1</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">d</td><td width = "255px">HA1</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">e</td><td width = "255px">HA2</td><td width = "200px">50*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">f</td><td width = "255px">HA2</td><td width = "200px">52*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">g</td><td width = "255px">HA2</td><td width = "200px">54*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">h</td><td width = "255px">HA2</td><td width = "200px">56*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">i</td><td width = "255px">Proximal promoter</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">j</td><td width = "255px">Proximal promoter</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">k</td><td width = "255px">Proximal promoter</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">l</td><td width = "255px">Proximal promoter</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">m</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">n</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">o</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">p</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | [[Image:KAIST-exp26.png|360px]]
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | | + | |
- | | + | |
- | == 9/17 PCR result ==
| + | |
- | | + | |
- | <span style=font-size:12px><b>PCR composition </b></span>
| + | |
- | <table border=1>
| + | |
- | <tr align=center>
| + | |
- | <td><b>Materials</b></td><td><b>Amount</b></td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td>Template</td><td>100pmole</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td>Primer A</td><td>50pmole</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td>Primer B</td><td>50pmole</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td>Bioneer Accupower HF PCR premix </td><td> < 1ul</td>
| + | |
- | </tr>
| + | |
- | <tr align=center><td>DW (3rd sterile water)</td><td>rest</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td>Total</td><td>50ul</td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | <b>Exp 1</b>
| + | |
- | <table border=1>
| + | |
- | <tr align=center>
| + | |
- | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | | + | |
- | <table border=1>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">a</td><td width = "255px">Selection marker(41)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">b</td><td width = "255px">Selection marker(41)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">c</td><td width = "255px">Selection marker(41)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">d</td><td width = "255px">Selection marker(41)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">e</td><td width = "255px">Selection marker(42)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">f</td><td width = "255px">Selection marker(42)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">g</td><td width = "255px">Selection marker(42)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">h</td><td width = "255px">Selection marker(42)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">i</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">30%</td><td width = "70px">1107bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">j</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">50%</td><td width = "70px">1107bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">k</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>4
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">l</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | [[Image:KAIST-exp25.png|360px]]
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | <b>Exp 2</b>
| + | |
- | <table border=1>
| + | |
- | <tr align=center>
| + | |
- | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | <table border=1>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">a</td><td width = "255px">HA1</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">b</td><td width = "255px">HA1</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">c</td><td width = "255px">HA1</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">d</td><td width = "255px">HA1</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">e</td><td width = "255px">HA2</td><td width = "200px">50*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">f</td><td width = "255px">HA2</td><td width = "200px">52*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">g</td><td width = "255px">HA2</td><td width = "200px">54*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">h</td><td width = "255px">HA2</td><td width = "200px">56*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">i</td><td width = "255px">Proximal promoter</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">j</td><td width = "255px">Proximal promoter</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">k</td><td width = "255px">Proximal promoter</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">l</td><td width = "255px">Proximal promoter</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">m</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">n</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">o</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td>
| + | |
- | </tr>
| + | |
- | <tr align=center>
| + | |
- | <td width = "40px">p</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | [[Image:KAIST-exp26.png|360px]]
| + | |
| <br> | | <br> |
| <br> | | <br> |