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Week13 9/5/10-9/11/10
From 2010.igem.org
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Kevin did some reorganizing to get the lab back in order for Prof. Mordacq's class this fall. He consolidated the bench space, the -20 refrigerator and the cold room. | Kevin did some reorganizing to get the lab back in order for Prof. Mordacq's class this fall. He consolidated the bench space, the -20 refrigerator and the cold room. | ||
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===9/7/10=== | ===9/7/10=== | ||
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===9/8/10=== | ===9/8/10=== | ||
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===9/9/10=== | ===9/9/10=== | ||
Latest revision as of 02:10, 27 October 2010
Week13 Highlights
We began reorganizing our lab space and equipment in preparation for a fall quarter class that would be utilizing the majority of the lab. We attempted standard assembly of LacP-RBS-CHS3 because the presence of a P site in CHS3 prevented 3A assembly.
9/6/10
Kevin did some reorganizing to get the lab back in order for Prof. Mordacq's class this fall. He consolidated the bench space, the -20 refrigerator and the cold room.
9/7/10
Day Off
9/8/10
Day Off
9/9/10
Assembled LacP-RBS1-CHS3 in a Chlor backbone plasmid using standard assembly methods Cut LacP-RBS1 using S Cut CHS3 using X and S (50% chance of inserting backwards --> must select for this after)
9/10/10
Inoculated 20 LacP-RBS-CHS3 cells.
Assembled CP-LacPI-RFP parts into Tet backbone.
