Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/14

From 2010.igem.org

(Difference between revisions)
(→1:PCR)
 
(16 intermediate revisions not shown)
Line 1: Line 1:
 +
{{:Team:Tokyo_Metropolitan/Notebook/Pattern}}
 +
=2010/10/14=
=2010/10/14=
-
 
===1:Electrophoresis===
===1:Electrophoresis===
Line 7: Line 8:
<Sample><br />
<Sample><br />
-
*1-2-7
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2-7]
-
*1-2
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2]
-
*10-2-7
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2-7]
-
*9-7
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7]
-
*4-5
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5]
-
 
+
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
-
 
<Result><br />
<Result><br />
[[Image:Matsuura_2010-10-14_14hr_29min.JPG]]
[[Image:Matsuura_2010-10-14_14hr_29min.JPG]]
 +
===2：DNA extraction===
===2：DNA extraction===
Line 26: Line 26:
＜Sample＞<br />
＜Sample＞<br />
-
*1-2-7
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2-7]
-
*10-2-7
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2-7]
-
*9-7
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7]
＜Protocol＞<br />
＜Protocol＞<br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
 +
===3：DNA Ligation ===
===3：DNA Ligation ===
Line 38: Line 39:
＜Sample＞<br />
＜Sample＞<br />
-
*4-5
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5]
-
*1-2-3
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2-3]
-
*10-2-3  
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2-3]
-
*10-2-9
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2-9]
-
*7-8-10  
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8-10]
(after digestion)
(after digestion)
＜Protocol＞<br />
＜Protocol＞<br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ]
 +
===4：DNA Digestion===
===4：DNA Digestion===
Line 62: Line 64:
＜Protocol＞<br />
＜Protocol＞<br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]
 +
===5:PCR===
===5:PCR===
Line 69: Line 72:
<Sample><br />
<Sample><br />
*vector
*vector
-
*4-5
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5]
-
*1-2-3
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2-3]
-
*10-2-3
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2-3]
-
*7-8-10
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8-10]
-
*10-2-9
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2-9]
-
 
+
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ]
 +
===6：DNA extraction from gels===
===6：DNA extraction from gels===
Line 84: Line 87:
＜Sample＞<br />
＜Sample＞<br />
-
*9-7
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7]
-
 
+
(After electrophoresis)
(After electrophoresis)
-
 
＜Protocol＞<br />
＜Protocol＞<br />
See[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
See[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
 +
===7：DNA Digestion===
===7：DNA Digestion===
Line 98: Line 100:
＜Sample, Materials＞<br />
＜Sample, Materials＞<br />
・PCR productions
・PCR productions
-
*1-2
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2]
-
*1-2-7
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2-7]
-
*10-2-7
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2-7]
・Digest enzyme
・Digest enzyme
Line 110: Line 112:
＜Protocol＞<br />
＜Protocol＞<br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]
 +
===8:PCR===
===8:PCR===
Line 116: Line 119:
<Sample><br />
<Sample><br />
-
*9
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9]
-
*9-7
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7]
-
 
+
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ]
-
===7：DNA extraction from gels===
+
 
 +
===9：DNA extraction from gels===
＜Member＞<br />
＜Member＞<br />
-
 
+
nito
-
 
+
＜Sample＞<br />
＜Sample＞<br />
-
*1+2(8/31) 
+
*9-7
-
*4+5(8/31)
+
-
 
+
(After electrophoresis)
(After electrophoresis)
Line 139: Line 139:
-
===3:Electrophoresis===
+
===10:Electrophoresis===
<Member><br />
<Member><br />
-
mio,mizuki
+
nito
<Sample><br />
<Sample><br />
-
PCR products (9/16)
+
*vector
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7]
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5]
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
-
 
<Result><br />
<Result><br />
-
[[Image:Matsuura_2010-09-16_18hr_19min.JPG]]
+
[[Image:Matsuura_2010-10-14_22hr_56min.JPG]]
 +
 
 +
 
 +
===11：DNA extraction from gels===
 +
＜Member＞<br />
 +
nito
 +
 
 +
＜Sample＞<br />
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7] 
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5]
 +
(After electrophoresis)
 +
 
 +
＜Protocol＞<br />
 +
See[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]

Latest revision as of 02:59, 25 October 2010


E.coli Pattern Formation Project Notebook



August 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
September 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October 2010
SUNMONTUEWEDTHUFRISAT
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

Contents

2010/10/14

1:Electrophoresis

<Member>
nito

<Sample>

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-10-14 14hr 29min.JPG


2：DNA extraction

＜Member＞
nito

＜Sample＞

＜Protocol＞
See Protocol 4


3：DNA Ligation

＜Member＞
nito

＜Sample＞

(after digestion)

＜Protocol＞
See Protocol 10


4：DNA Digestion

＜Member＞
nito

＜Sample, Materials＞
・PCR productions

  • plasmid

・Digest enzyme

  • XbaⅠ
  • SpeⅠ

＜Protocol＞
See Prptpcpl 9


5:PCR

<Member>
nito

<Sample>

<Protocol>
See Protocol 1


6：DNA extraction from gels

＜Member＞
nito

＜Sample＞

(After electrophoresis)

＜Protocol＞
SeeProtocol 4


7：DNA Digestion

＜Member＞
nito

＜Sample, Materials＞
・PCR productions

・Digest enzyme

  • AvrⅡ
  • NheⅠ
  • XbaⅠ
  • SpeⅠ

＜Protocol＞
See Prptpcpl 9


8:PCR

<Member>
nito

<Sample>

<Protocol>
See Protocol 1


9：DNA extraction from gels

＜Member＞
nito

＜Sample＞

  • 9-7

(After electrophoresis)


＜Protocol＞
SeeProtocol 4


10:Electrophoresis

<Member>
nito

<Sample>

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-10-14 22hr 56min.JPG


11：DNA extraction from gels

＜Member＞
nito

＜Sample＞

(After electrophoresis)

＜Protocol＞
SeeProtocol 4

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Pattern/2010/10/14"