Team:IIT Delhi 1

From 2010.igem.org

(Difference between revisions)
 
(21 intermediate revisions not shown)
Line 1: Line 1:
<html>
<html>
-
 
-
<head>
 
<style>
<style>
Line 557: Line 555:
<div style='mso-element:para-border-div;border:none;border-bottom:solid #4F81BD 1.0pt;
<div style='mso-element:para-border-div;border:none;border-bottom:solid #4F81BD 1.0pt;
-
mso-border-bottom-themecolor:accent1;padding:0cm 0cm 4.0pt 0cm'>
+
mso-border-bottom-themecolor:accent1;padding:0cm 1cm 4.0pt 0cm'>
-
<p class=MsoTitle align=center style='text-align:center'><span lang=EN-US>IIT
+
<p align=center><embed src="https://static.igem.org/mediawiki/igem.org/5/51/Nish1.swf" width=900 height=500></p>
-
Delhi iGEM Wiki</span></p>
+
-
 
+
-
</div>
+
-
 
+
-
<p class=MsoNormal><span lang=EN-US><o:p>&nbsp;</o:p></span></p>
+
-
 
+
-
<h2><u><span lang=EN-US>Renin Angiotensin Aldosterone System (RAAS)<o:p></o:p></span></u></h2>
+
-
 
+
-
<p class=MsoNormal><span lang=EN-US><o:p>&nbsp;</o:p></span></p>
+
-
 
+
-
<h3><span lang=EN-US>Introduction</span></h3>
+
-
 
+
-
<p class=MsoNormal style='text-align:justify'><span lang=EN-US>The Renin
+
-
Angiotensin Aldosterone System is a hormone system in the human body that is
+
-
responsible for the regulation of blood pressure and fluid balance.</span></p>
+
-
 
+
-
<h3 style='text-align:justify'><span lang=EN-US>Functioning</span></h3>
+
-
 
+
-
<p class=MsoNormal style='text-align:justify'><span lang=EN-US>A decrease in
+
-
renal perfusion causes the release of the enzyme renin which subsequently
+
-
cleaves angiotensinogen to angiotensin I. This is further converted to
+
-
angiotensin II by angiotensin-converting enzyme (ACE). The bioactive product,
+
-
angiotensin II, causes the constriction of blood vessels, leading to an
+
-
increased blood pressure. It is also responsible for the secretion of
+
-
aldosterone by the adrenal cortex, which acts by increasing the re-absorption
+
-
of sodium and water into the blood. Hence, the fluid volume as well as the
+
-
blood pressure is maintained in the body.</span></p>
+
-
 
+
-
<h2><u><span lang=EN-US>The &#945;-hemolysin System in <i style='mso-bidi-font-style:
+
-
normal'>E.coli<o:p></o:p></i></span></u></h2>
+
-
 
+
-
<p class=MsoNormal><span lang=EN-US><o:p>&nbsp;</o:p></span></p>
+
-
 
+
-
<h3><span lang=EN-US>Introduction</span></h3>
+
-
 
+
-
<p class=MsoNormal style='text-align:justify'><span lang=EN-US>The
+
-
alpha-hemolysin system is a type I secretion system found in wild type <i
+
-
style='mso-bidi-font-style:normal'>E.coli </i>which enable them to cause
+
-
infection in the urinary tract and is an important virulence factor because of
+
-
its cytotoxic and cytolytic activity against a variety of mammalian cells. </span></p>
+
-
 
+
-
<h3 style='text-align:justify'><span lang=EN-US>Functioning</span></h3>
+
-
 
+
-
<p class=MsoNormal style='text-align:justify'><span lang=EN-US>The hemolysin
+
-
system originates from a series of hly genes. These genes are present in the
+
-
series <i style='mso-bidi-font-style:normal'>hlyC – hlyA – hlyB – hlyD</i>. Of
+
-
these, <i style='mso-bidi-font-style:normal'>hlyA</i> codes for the protein
+
-
that is responsible for the infection. Hly B and D are membrane proteins that
+
-
work in coordination for the secretion of HlyA. The synthesis, activation and
+
-
secretion of HlyA in <i style='mso-bidi-font-style:normal'>E.coli </i>is
+
-
determined by the <i style='mso-bidi-font-style:normal'>hlyCABD</i> operon. This
+
-
operon is either located either on the chromosome or on transmissible plasmids.
+
-
 
+
-
</span></p>
+
-
 
+
-
<p class=MsoNormal style='text-align:justify'><span lang=EN-US>It has been
+
-
shown that this system can be used to produce and secrete a protein of our
+
-
choice if the protein contains 60 amino acid residues from the carboxy terminal
+
-
end of the HlyA protein. </span></p>
+
-
 
+
-
<h2><u><span lang=EN-US>Our iGEM 2010 Project<o:p></o:p></span></u></h2>
+
-
 
+
-
<p class=MsoNormal><span lang=EN-US><o:p>&nbsp;</o:p></span></p>
+
-
 
+
-
<h3><span lang=EN-US>Introduction</span></h3>
+
-
 
+
-
<p class=MsoNormal style='text-align:justify'><span lang=EN-US>Our aim is to
+
-
create an online elicitor detection and response in a flow system, which can
+
-
function in the detection of high blood pressure and help reduce it by
+
-
releasing renin inhibitors into the circulatory system. This kind of a system
+
-
would be helpful to patients with a hypertensive condition. </span></p>
+
-
 
+
-
<h3><span lang=EN-US>Approach</span></h3>
+
-
 
+
-
<p class=MsoNormal><span lang=EN-US>Since the blood pressure system is not
+
-
really a chemical signal, what we have assumed is that the blood pressure can
+
-
be easily converted into a chemical signal. Having assumed this, we will use a
+
-
chemical signal such as IPTG or AHL as the elicitor to produce appropriate
+
-
amounts of renin inhibitor. For easy detection and verification, we are
+
-
planning to use GFP in place of renin inhibitor which can easily be replaced
+
-
later.</span></p>
+
-
 
+
-
<p class=MsoNormal><span lang=EN-US>For this we will be introducing two vectors
+
-
into <i style='mso-bidi-font-style:normal'>E. coli </i>as shown below.</span></p>
+
-
 
+
-
<p class=MsoNormal><span style='mso-ansi-language:EN-IN;mso-fareast-language:
+
-
EN-IN;mso-bidi-language:HI;mso-no-proof:yes'><!--[if gte vml 1]><v:shapetype
+
-
id="_x0000_t75" coordsize="21600,21600" o:spt="75" o:preferrelative="t"
+
-
path="m@4@5l@4@11@9@11@9@5xe" filled="f" stroked="f">
+
-
<v:stroke joinstyle="miter"/>
+
-
<v:formulas>
+
-
  <v:f eqn="if lineDrawn pixelLineWidth 0"/>
+
-
  <v:f eqn="sum @0 1 0"/>
+
-
  <v:f eqn="sum 0 0 @1"/>
+
-
  <v:f eqn="prod @2 1 2"/>
+
-
  <v:f eqn="prod @3 21600 pixelWidth"/>
+
-
  <v:f eqn="prod @3 21600 pixelHeight"/>
+
-
  <v:f eqn="sum @0 0 1"/>
+
-
  <v:f eqn="prod @6 1 2"/>
+
-
  <v:f eqn="prod @7 21600 pixelWidth"/>
+
-
  <v:f eqn="sum @8 21600 0"/>
+
-
  <v:f eqn="prod @7 21600 pixelHeight"/>
+
-
  <v:f eqn="sum @10 21600 0"/>
+
-
</v:formulas>
+
-
<v:path o:extrusionok="f" gradientshapeok="t" o:connecttype="rect"/>
+
-
<o:lock v:ext="edit" aspectratio="t"/>
+
-
</v:shapetype><v:shape id="Picture_x0020_0" o:spid="_x0000_i1026" type="#_x0000_t75"
+
-
alt="Vector1.png" style='width:195pt;height:209.25pt;visibility:visible;
+
-
mso-wrap-style:square'>
+
-
<v:imagedata src="IIT+Delhi+iGEM+Wiki_files/image001.png" o:title="Vector1"/>
+
-
</v:shape><![endif]--><![if !vml]><img width=260 height=279
+
-
src="IIT+Delhi+iGEM+Wiki_files/image002.gif" alt=Vector1.png v:shapes="Picture_x0020_0"><![endif]></span><span
+
-
lang=EN-US><span style='mso-spacerun:yes'>              </span><span
+
-
style='mso-tab-count:1'>          </span></span><span style='mso-ansi-language:
+
-
EN-IN;mso-fareast-language:EN-IN;mso-bidi-language:HI;mso-no-proof:yes'><!--[if gte vml 1]><v:shape
+
-
id="Picture_x0020_1" o:spid="_x0000_i1025" type="#_x0000_t75" alt="Vector2.png"
+
-
style='width:195pt;height:212.25pt;visibility:visible;mso-wrap-style:square'>
+
-
<v:imagedata src="IIT+Delhi+iGEM+Wiki_files/image003.png" o:title="Vector2"/>
+
-
</v:shape><![endif]--><![if !vml]><img width=260 height=283
+
-
src="IIT+Delhi+iGEM+Wiki_files/image004.gif" alt=Vector2.png v:shapes="Picture_x0020_1"><![endif]></span></p>
+
-
 
+
-
<p class=MsoNormal><span lang=EN-US><o:p>&nbsp;</o:p></span></p>
+
-
 
+
-
<p class=MsoNormal style='text-align:justify'><span lang=EN-US>The first vector
+
-
which contains the GFP-hlyA chimeric sequence with pLac promoter is used to
+
-
produce the chimera GFP protein which contains 62 amino acid residues from the
+
-
C terminal of hlyA protein, a prerequisite for the secretion of the protein
+
-
outside the cell body. Forming a chimera will not in any way change the
+
-
structure or function as per literature.</span></p>
+
-
 
+
-
<p class=MsoNormal><span lang=EN-US><o:p>&nbsp;</o:p></span></p>
+
-
 
+
-
<p class=MsoNormal><span lang=EN-US>The other vector contains the hlyB and hlyD
+
-
sequences with ptet promoter sequence. These two proteins will be
+
-
constitutively expressed. These proteins are present in the membrane and
+
-
provide for an outlet of hlyA or chimeras with 62 amino acid residues of the C
+
-
terminal of hlyA.</span></p>
+
-
 
+
-
<p class=MsoNormal><span lang=EN-US>After we are successful in obtaining
+
-
GFP-hlyA chimera outside the cell body, our next task will be to immobilize
+
-
these cells in a flow system and characterize the flow-expression profile. We
+
-
would want to keep the cells in G0 phase so as to have continuous expression of
+
-
the protein without cell death</span></p>
+
-
 
+
-
<p class=MsoNormal><span lang=EN-US>We will also be working towards minimizing
+
-
the toxins and other products so that such a system can finally be thought fit
+
-
for incorporating and integrating with the circulatory system in our body.</span></p>
+
-
 
+
-
<p class=MsoNormal><span lang=EN-US><o:p>&nbsp;</o:p></span></p>
+
-
 
+
-
<p class=MsoNormal style='text-align:justify'><span lang=EN-US><o:p>&nbsp;</o:p></span></p>
+
 +
<p><h2>Special comments:</h2>
 +
1.Plasmids acquired from Karolinska Institutet, Solna, Sweden. <br>
 +
2.Safety page in 'Work' link.
</div>
</div>
Line 717: Line 567:
</html>
</html>
-
 
-
 
-
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
 
-
!align="center"|[[Team:IIT_Delhi_1|Home]]
 
-
!align="center"|[[Team:IIT_Delhi_1/Team|Team]]
 
-
!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=IIT_Delhi_1 Official Team Profile]
 
-
!align="center"|[[Team:IIT_Delhi_1/Project|Project]]
 
-
!align="center"|[[Team:IIT_Delhi_1/Parts|Parts Submitted to the Registry]]
 
-
!align="center"|[[Team:IIT_Delhi_1/Modeling|Modeling]]
 
-
!align="center"|[[Team:IIT_Delhi_1/Notebook|Notebook]]
 
-
!align="center"|[[Team:IIT_Delhi_1/Safety|Safety]]
 
-
|}
 

Latest revision as of 01:05, 28 October 2010

Special comments:

1.Plasmids acquired from Karolinska Institutet, Solna, Sweden.
2.Safety page in 'Work' link.

Retrieved from "http://2010.igem.org/Team:IIT_Delhi_1"