Latest revision as of 19:20, 27 October 2010

Promoters

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The Nevada team has created environmental stress sensors by using rd29A and DREB1C promoters to express red fluorescent protein and other bio-fluorescent markers. When induced by environmental stress, plants carrying these genes can easily be detected by the farmer walking through his field or by a plane flying over acres of farmland. Promoter elements are short and easy to work with. They also allow for modification and specialization. DREB1C and rd29A both have multiple binding motifs in their promoter regions allowing for variation in expression levels and the particular stresses that induce them. 35S is a constitutive promoter that can be valuable for control groups in stress and other plant response research.

Fluorescent Plant Image Taken From: http://www.edinformatics.com/inventions_inventors/genetic_engineering.htm

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-{{nevadaparts}}
+{{Nevada_css}}
+[[Image:Promoters UNR Final.png|border|left|950px]]
+{{Nevada_topbar}}
+<div style="padding: 10px 10px 30px 10px;">
-== Promoters ==
+<p>&nbsp;</p>
+== Promoters ==
-{|align="justify"
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 <h1>Subpages</h1>
 <ul>
-<li><a href="https://2010.igem.org/Team:Nevada/DREB1C" tabindex="1">DREB 1C</a></li>
+<li><a href="https://2010.igem.org/Team:Nevada/DREB1C" tabindex="1">DREB1C</a></li>
-<li><a href="https://2010.igem.org/Team:Nevada/RD29A" tabindex="2">RD29A</a></li>
+<li><a href="https://2010.igem.org/Team:Nevada/RD29A" tabindex="2">rd29A</a></li>
 <li><a href="https://2010.igem.org/Team:Nevada/35S" tabindex="3">35S</a></li>
-<li><a href="https://2010.igem.org/Team:Nevada/CD2+Inducible" tabindex="4">CD2+ Inducible</a></li>
+<li><a href="https://2010.igem.org/Team:Nevada/CD2Inducible" tabindex="4">CD2+ Inducible</a></li>
 </ul>
 </div>
 </html>
-<p>The 2010 Nevada iGEM team has three goals for this year’s competition.  First, we are going to test the validity of utilizing Nicotiana tabacum protoplasts (NT cells), plant cells without the cell wall, as a model for the expression of higher plant genes for future iGEM competitions.  This system is useful in the respect that the time it takes to obtain transgenic lines of cells is greatly reduced compared to the time to obtain transgenic plants.  <html>
+<html>
+<body link=#00ffff>
 <a href="https://static.igem.org/mediawiki/2010/7/79/GFP_tobacco_plant.jpg"><img src="https://static.igem.org/mediawiki/2010/7/79/GFP_tobacco_plant.jpg" class="shadow" style="float:left;width:170px;margin:10px"></a>
-</html>These cells can therefore be utilized as a quick proof-of-concept test model before moving synthetic constructs into plants of interest.  We also aim to produce an iGEM-compatible plant-specific plasmid, several stress-inducible plant promoters, reporter genes containing Kozak sequences (ribosome binding sites) and terminators that conform to BioBrick standards.  Lastly, we hope to measure the induction of these stress promoters in real-time by performing a fluorometry assay in which stress will be applied to NT cells and fluorescent output by a reporter (GFP) will be measured to detail induction in real time.  This method has a distinct advantage over microarrays since microarrays are only one ‘snapshot’ in time.</p>
+</html><p>The Nevada team has created environmental stress sensors by using <html><a href="https://2010.igem.org/Team:Nevada/RD29APromoter"><span style="color:#1569C7;font-weight:bold;">rd29A</span></a></html> and <html><a href="https://2010.igem.org/Team:Nevada/DREB1CPromoter"><span style="color:#1569C7;font-weight:bold;">DREB1C</span></a></html> promoters to express red fluorescent protein and other bio-fluorescent markers. When induced by environmental stress, plants carrying these genes can easily be detected by the farmer walking through his field or by a plane flying over acres of farmland. Promoter elements are short and easy to work with. They also allow for modification and specialization. <html><a href="https://2010.igem.org/Team:Nevada/DREB1CPromoter"><span style="color:#1569C7;font-weight:bold;">DREB1C</span></a></html> and <html><a href="https://2010.igem.org/Team:Nevada/RD29APromoter"><span style="color:#1569C7;font-weight:bold;">rd29A</span></a></html> both have multiple binding motifs in their promoter regions allowing for variation in expression levels and the particular stresses that induce them. 35S is a constitutive promoter that can be valuable for control groups in stress and other plant response research.</p>
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+<p>&nbsp;</p>
-|}
-<!--- The Mission, Experiments --->
+<p>Fluorescent Plant Image Taken From: http://www.edinformatics.com/inventions_inventors/genetic_engineering.htm</p>
-'''We would like to thank the following sponsors for their support in helping us make this project possible.'''
-Much thanks to the [http://www.cabnr.unr.edu/Students/Majors.aspx Departments of Biochemistry and Biotechnology] and the [http://www.cabnr.unr.edu/Students/Glance.aspx College of Agriculture, Biotechnology and Natural Resources] for their encouragement and support. Thank you [http://www.unr.edu/inbre/ Nevada INBRE] for over $6,000 in support for supplies and registration costs.
-Thank you to [https://asun.unr.edu/Default.aspx Associated Students of the Univeristy of Nevada] for supporting our fund raising efforts.
-Thank you to [http://www.promega.com/Catalog/CountrySelect.aspx?returnurl=/Default.asp Promega Co.] for free enzyme donations.
-Thank you to [http://www.invitrogen.com/site/us/en/home.html?cid=covinvggl89100000002336s& Invitrogen Co.] for a discount on our Vector NTI program.
 {| style="color:#FFFFFF;background-color:#FFFFFF;" cellpadding="3" cellspacing="1" border="1" bordercolor="#008000" width="100%" align="center"
 !align="center"|[[Image:Nevada_CABNR.jpg|200px]]
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 !align="center"|[[Image:Promega_logo.jpg‎]]
 !align="center"|[[Image:Invitrogen_logo.jpeg]]
+!align="center"|[[Image:Sda logo small.jpg]]
 |}

Team:Nevada/promoters

From 2010.igem.org

Latest revision as of 19:20, 27 October 2010

Promoters

Subpages