Team:Harvard/allergy/parts

From 2010.igem.org

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<tr><td><b>Name</b></td><td><b>Original Name</b></td><td><b>Description</b></td><td><b>Resistance</b></td><td><b>Sequence</b></td></tr>
<tr><td><b>Name</b></td><td><b>Original Name</b></td><td><b>Description</b></td><td><b>Resistance</b></td><td><b>Sequence</b></td></tr>
<tr><td>A1</td><td>amiRNA GFP</td><td>GFP knockdown construct</td><td>chloramphenicol</td><td><a href="AmirnaGFP.txt"> amirnaGFP</a></td></tr>
<tr><td>A1</td><td>amiRNA GFP</td><td>GFP knockdown construct</td><td>chloramphenicol</td><td><a href="AmirnaGFP.txt"> amirnaGFP</a></td></tr>
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<tr><td>A2</td><td>amiRNA (Other allergen x)</td><td>knockdown construct for x </td><td>chloramphenicol</td><td> <a href="insert">amiRNA x</a></td></tr>
+
<tr><td>A2</td><td>amiRNA Bet </td><td>Bet knockdown construct</td><td>chloramphenicol</td><td> <a href="Bet1.txt">amiRNA Bet</a></td></tr>
<tr><td>A3</td><td>ihpRNA Bet</td><td>Intron hairpin Bet Knockdown construct</td><td>chloramphenicol</td><td><a href="Bet1.txt"> ihpRNA Bet1</a></td></tr>
<tr><td>A3</td><td>ihpRNA Bet</td><td>Intron hairpin Bet Knockdown construct</td><td>chloramphenicol</td><td><a href="Bet1.txt"> ihpRNA Bet1</a></td></tr>
<tr><td>A4</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>chloramphenicol</td><td><a href="LTP.txt">ihpRNA LTP</a></td></tr>
<tr><td>A4</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>chloramphenicol</td><td><a href="LTP.txt">ihpRNA LTP</a></td></tr>

Revision as of 08:03, 13 October 2010



parts and primers

Allergy

For more information on the allergens visit our "meet the allergens" page

NameOriginal NameDescriptionResistanceSequence
A1amiRNA GFPGFP knockdown constructchloramphenicol amirnaGFP
A2amiRNA Bet Bet knockdown constructchloramphenicol amiRNA Bet
A3ihpRNA BetIntron hairpin Bet Knockdown constructchloramphenicol ihpRNA Bet1
A4ihpRNA LTPIntron hairpin LTP Knockdown constructchloramphenicolihpRNA LTP
A5ihpRNA Ger Intron hairpin Ger Knockdown constructchloramphenicolihpRNA GER


Primers

NameDescriptionSequence
AP11 of 4 primers used to create the amiRNA GFP construct GFP primer1
AP22 of 4 primers used to create the amiRNA GFP construct GFP primer2
AP33 of 4 primers used to create the amiRNA GFP construct GFP primer3
AP44 of 4 primers used to create the amiRNA GFP construct GFP primer4
AP51 of 4 primers used to create the amiRNA Bet construct Bet1 Primer 1
AP62 of 4 primers used to create the amiRNA Bet construct Bet1 Primer 2
AP73 of 4 primers used to create the amiRNA Bet construct Bet1 Primer 3
AP84 of 4 primers used to create the amiRNA Bet construct Bet1 Primer 4
AP9Forward primer for Bet v1 Sense Bet1 Sense Forward
AP10Reverse primer for Bet v1 Sense Bet1 Sense Reverse
AP11Forward primer for Bet v1 AntisenseBet1 Antisense Forward
AP12Reverse primer for Bet v1 Antisense Bet1 Antisense Reverse
AP13Forward primer for LTP Sense LTP Sense Forward
AP14Reverse primer for LTP SenseLTP Sense Reverse
AP15Forward primer for LTP AntisenseLTP Antisense Forward
AP16Reverse primer for LTP AntisenseLTP Antisense Reverse
AP17Forward primer for Ger SenseGer Sense Forward
AP18Reverse primer for Ger SenseGer Sense Reverse
AP19Forward primer for Ger AntisenseGer Antisense Forward
AP20Reverse primer for Ger AntisenseGer Antisense Reverse
AP21Forward Primer for PDK intron PDK forward
AP22Reverse Primer for PDK intron PDK reverse
AP23Forward Primer to make amiRNA Biobrick compatible Biobrick forward
AP24Reverse Primer to make amiRNA Biobrick compatible Biobrick reverse
AP251 of 4 primers used to create the amiRNA LTP constructLTP primer 1
AP262 of 4 primers used to create the amiRNA LTP constructLTP primer 2
AP273 of 4 primers used to create the amiRNA LTP constructLTP primer 3
AP284 of 4 primers used to create the amiRNA LTP constructLTP primer 4

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