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Team:Harvard/allergy/parts
From 2010.igem.org
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<tr><td><b>Name</b></td><td><b>Description</b></td><td><b>Sequence</b></td></tr> | <tr><td><b>Name</b></td><td><b>Description</b></td><td><b>Sequence</b></td></tr> | ||
| - | <tr><td> | + | <tr><td>AP1</td><td>1 of 4 primers used to create the amiRNA GFP construct</td><td><a href="AmirnaGFP.txt"> primer1</a> </td></tr> |
| - | <tr><td> | + | <tr><td>AP2</td><td>2 of 4 primers used to create the amiRNA GFP construct</td><td><a href="AmirnaGFP.txt"> primer2</a></td></tr> |
| - | <tr><td> | + | <tr><td>AP3</td><td>3 of 4 primers used to create the amiRNA GFP construct</td><td><a href="AmirnaGFP.txt"> primer3</a></td></tr> |
| - | <tr><td> | + | <tr><td>AP4</td><td>4 of 4 primers used to create the amiRNA GFP construct</td><td><a href="AmirnaGFP.txt"> primer4</a></td></tr> |
<tr><td>VP7</td><td>HindIII-biobrick multiple cloning site-smGFP-forward primer for Reporter Series 1 insert</td><td></td></tr> | <tr><td>VP7</td><td>HindIII-biobrick multiple cloning site-smGFP-forward primer for Reporter Series 1 insert</td><td></td></tr> | ||
<tr><td>VP8</td><td>smGFP-NheI reverse primer for Reporter Series 1 insert</td><td></td></tr> | <tr><td>VP8</td><td>smGFP-NheI reverse primer for Reporter Series 1 insert</td><td></td></tr> | ||
Revision as of 07:01, 13 October 2010
parts and primers
Allergy
For more information on the allergens visit our "meet the allergens" page| Name | Original Name | Description | Resistance | Sequence |
| A1 | amiRNA GFP | GFP knockdown construct | chloramphenicol | amirnaGFP |
| A2 | amiRNA (Other allergen x) | knockdown construct for x | chloramphenicol | amiRNA x |
| A3 | ihpRNA Bet | Intron hairpin Bet Knockdown construct | chloramphenicol | ihpRNA Bet1 |
| A4 | ihpRNA LTP | Intron hairpin LTP Knockdown construct | chloramphenicol | ihpRNA LTP |
| A5 | ihpRNA Ger | Intron hairpin Ger Knockdown construct | chloramphenicol | ihpRNA GER |
Primers
| Name | Description | Sequence |
| AP1 | 1 of 4 primers used to create the amiRNA GFP construct | primer1 |
| AP2 | 2 of 4 primers used to create the amiRNA GFP construct | primer2 |
| AP3 | 3 of 4 primers used to create the amiRNA GFP construct | primer3 |
| AP4 | 4 of 4 primers used to create the amiRNA GFP construct | primer4 |
| VP7 | HindIII-biobrick multiple cloning site-smGFP-forward primer for Reporter Series 1 insert | |
| VP8 | smGFP-NheI reverse primer for Reporter Series 1 insert | |
| VP9 | Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert | |
| VP11 | Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert | |
| VP12 | 1 of 5 for sequencing V12 biobrick cloning site and smgfp reporter | |
| VP13 | 2 of 5 for sequencing V12 biobrick cloning site and smgfp reporter | |
| VP14 | 3 of 5 for sequencing V12 biobrick cloning site and smgfp reporter | |
| VP15 | 4 of 5 for sequencing V12 biobrick cloning site and smgfp reporter | |
| VP16 | 5 of 5 for sequencing V12 biobrick cloning site and smgfp reporter | |
| VP17 | 1 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
| VP18 | 2 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
| VP19 | 3 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
| VP20 | 4 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
| VP21 | 5 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
| VP22 | 6 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
| VP23 | 7 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
| VP24 | 8 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
| VP25 | 9 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
| VP26 | 1 of 5 for sequencing V9 and V10 biobrick cloning site and promoter | |
| VP27 | 2 of 5 for sequencing V9 and V10 biobrick cloning site and promoter | |
| VP28 | 3 of 5 for sequencing V9 and V10 biobrick cloning site and promoter | |
| VP29 | 4 of 5 for sequencing V9 and V10 biobrick cloning site and promoter | |
| VP30 | 5 of 5 for sequencing V9 and V10 biobrick cloning site and promoter | |
| VP33 | 1 of 2 for sequencing V7 and V8 biobrick cloning site | |
| VP34 | 2 of 2 for sequencing V7 and V8 biobrick cloning site |
