Team:Harvard/allergy/parts

From 2010.igem.org

(Difference between revisions)
Line 1: Line 1:
{{harvard_css}}
{{harvard_css}}
{{harvard_allergy}}
{{harvard_allergy}}
 +
 +
<html>
<html>
 +
 +
<style type="text/css">
 +
 +
table
 +
{
 +
border-collapse:collapse;
 +
font-size:10pt;
 +
}
 +
 +
 +
td
 +
{
 +
padding-left: 5px;
 +
padding-right: 5px;
 +
padding-top: 2px;
 +
padding-bottom: 2px;
 +
border-style: solid;
 +
border-color: #827B60;
 +
border-width: 1px;
 +
}
 +
 +
</style>
</div>
</div>

Revision as of 06:21, 13 October 2010



parts and primers

parts and primers

Allergy

For more information on the vectors visit our "meet the allergens" page

NameOriginal NameDescriptionResistanceSequence
A1amiRNA GFPGFP knockdown constructchloramphenicol amirnaGFP
A2amiRNA (Other allergen x)knockdown construct for x chloramphenicol amiRNA x
A3ihpRNA BetIntron hairpin Bet Knockdown constructchloramphenicol ihpRNA Bet1
A4ihpRNA LTPIntron hairpin LTP Knockdown constructchloramphenicolihpRNA LTP
A5ihpRNA Ger Intron hairpin Ger Knockdown constructchloramphenicolihpRNA GER


Primers

NameDescriptionSequence
VP3HindIII-pENTCUP2 forward primer for Expression Series insert
VP4pENTCUP2-biobrick multiple cloning site-NheI reverse primer for Expression Series insert
VP5HindIII-biobrick multiple cloning site-gusA-forward primer for Reporter Series 1 insert
VP6gusA-NheI reverse primer for Reporter Series 1 insert
VP7HindIII-biobrick multiple cloning site-smGFP-forward primer for Reporter Series 1 insert
VP8smGFP-NheI reverse primer for Reporter Series 1 insert
VP9Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert
VP11Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert
VP121 of 5 for sequencing V12 biobrick cloning site and smgfp reporter
VP132 of 5 for sequencing V12 biobrick cloning site and smgfp reporter
VP143 of 5 for sequencing V12 biobrick cloning site and smgfp reporter
VP154 of 5 for sequencing V12 biobrick cloning site and smgfp reporter
VP165 of 5 for sequencing V12 biobrick cloning site and smgfp reporter
VP171 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP182 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP193 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP204 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP215 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP226 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP237 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP248 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP259 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP261 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP272 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP283 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP294 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP305 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP331 of 2 for sequencing V7 and V8 biobrick cloning site
VP342 of 2 for sequencing V7 and V8 biobrick cloning site

Retrieved from "http://2010.igem.org/Team:Harvard/allergy/parts"