"

From 2010.igem.org

Revision as of 00:12, 8 October 2010 (view source) Dspakowicz (Talk | contribs) (→Oct 05, 2010; Making stock solutions) ← Older edit		Revision as of 17:17, 12 October 2010 (view source) Dspakowicz (Talk | contribs) (→Oct 07, 2010; Bacterial growth assays, Cu ppt) Newer edit →
Line 40:		Line 40:
	| 11||LB t4||10/7/2010 3:54:36 PM||600||1.064		| 11||LB t4||10/7/2010 3:54:36 PM||600||1.064
	|}		|}
		+
		+	===Oct 12, 2010; Standard curve troubleshooting===
	===Useful links===		===Useful links===

---

Revision as of 17:17, 12 October 2010

General description of goal of this part of the project.

Contents 1 Oct 06, 2010; Making stock solutions 2 Oct 07, 2010; Bacterial growth assays, Cu ppt 3 Oct 12, 2010; Standard curve troubleshooting 4 Useful links

Oct 06, 2010; Making stock solutions

Generated the stock solutions for the Followed the standard curve protocol.

• Made a 100 mM stock solution of BCS.
• Made two CuSO4 dilution series, in LB and MM, from 10 mM Cu to 10 pM.

Oct 07, 2010; Bacterial growth assays, Cu ppt

#	Sample ID	Date and Time	1 (nm)	1 (Abs)
1	o/n culture	10/7/2010 11:16:10 AM	600	0.854
2	MM t0	10/7/2010 11:44:45 AM	600	0.002
3	LB t0	10/7/2010 11:31:06 AM	600	0.089
4	LB t1	10/7/2010 12:47:55 PM	600	0.189
5	MM t1	10/7/2010 12:45:13 PM	600	0.002
6	MM t2	10/7/2010 1:41:51 PM	600	0.004
7	LB t2	10/7/2010 1:43:38 PM	600	0.450
8	MM t3	10/7/2010 2:44:50 PM	600	0.001
9	LB t3	10/7/2010 2:46:09 PM	600	0.761
10	MM t4	10/7/2010 3:53:12 PM	600	0.005
11	LB t4	10/7/2010 3:54:36 PM	600	1.064

Oct 12, 2010; Standard curve troubleshooting

Useful links

LabNotebook

[http://meta.wikimedia.org/wiki/Help:Wikitext_examples Wiki formatting help]

[http://excel2wiki.net/ Excel table to Wiki format tool]