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Team:WITS-South Africa/Machine Design
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Revision as of 13:36, 12 October 2010
Intermediate Machine 2 (Figure 1) Purpose: a) To characterise the strength of the induction of the LacI/AraC promoter by measuring fluorescence after IPTG is added b) To show that the PlcR promoter is activated in L. gasseri by measuring fluorescence after the addition of exogenous PlcR and PapR proteins
Fig1. The proposed intermediate Machine 2
Final Machine 1 (Figure 2) Purpose: a) To act as the ‘Detector’ Machine within the population and produce the quorum signalling peptide in response to an input signal
Fig 2. The proposed Final Machine 1
Final Machine 2 (Figure 3) Purpose a) To act as the ‘Reporter’ Machine within the population and respond to infection as previously described
Fig 3. The proposed Final Machine 2
