Team:Yale/Our Project/Notebook

From 2010.igem.org

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Lab Notebook: Week 1
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Monday 6/7--defended project (yay!)
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Wednesday 6/9--started in lab--got set up & started cultures of DH5alpha and LE392
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Thursday 6/10--inoculated liquid cultures in AM, made up different copper sulfate & LB solutions, then ran first copper growth assay in plate reader in PM w/three different ODs of each strain(4 hr. protocol)
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Friday 6/11--Ran another growth assay with middling concentrations based on results of first, but bacteria failed to grow well--b/c allowed to overgrow?
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Revision as of 06:50, 12 October 2010

iGEM Yale

our project

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Lab Notebook: Week 1

Monday 6/7--defended project (yay!) Wednesday 6/9--started in lab--got set up & started cultures of DH5alpha and LE392 Thursday 6/10--inoculated liquid cultures in AM, made up different copper sulfate & LB solutions, then ran first copper growth assay in plate reader in PM w/three different ODs of each strain(4 hr. protocol) Friday 6/11--Ran another growth assay with middling concentrations based on results of first, but bacteria failed to grow well--b/c allowed to overgrow?