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Team:Newcastle/15 June 2010
From 2010.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
'''Nanodrop protocol''' | '''Nanodrop protocol''' | ||
| - | Nanodrop can be used to measure the DNA, RNA and protein | + | Nanodrop can be used to measure the DNA, RNA and protein |
# Select Nanodrop program from the desktop | # Select Nanodrop program from the desktop | ||
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# If dealing with multiple samples, clean the equipment with water at regular intervals | # If dealing with multiple samples, clean the equipment with water at regular intervals | ||
# After measurement, clean the equipment with a drop of water on the spectrometer and press blank | # After measurement, clean the equipment with a drop of water on the spectrometer and press blank | ||
| + | |||
| + | '''Gel electrophoresis''' | ||
| + | |||
| + | Gel electrophoresis can used to separate DNA, RNA, or protein molecules by molecular weight using an electric field applied to a gel matrix | ||
Revision as of 08:54, 17 June 2010
Nanodrop protocol
Nanodrop can be used to measure the DNA, RNA and protein
- Select Nanodrop program from the desktop
- To clean Nanodrop, add a drop of water on the spectrometer and press blank
- After cleaning, wipe the water off
- To equalizen the equipment, add 3μl of the buffer used in the sample and press blank
- Wipe the buffer off
- To measure sample, add 3μl of the sample and press measure
- If dealing with multiple samples, clean the equipment with water at regular intervals
- After measurement, clean the equipment with a drop of water on the spectrometer and press blank
Gel electrophoresis
Gel electrophoresis can used to separate DNA, RNA, or protein molecules by molecular weight using an electric field applied to a gel matrix
