Team:Virginia United
From 2010.igem.org
Line 82: | Line 82: | ||
<li><a href="https://2010.igem.org/Team:Virginia_United/Notebook"rel="ddsubmenu4">NOTEBOOK</a></li> | <li><a href="https://2010.igem.org/Team:Virginia_United/Notebook"rel="ddsubmenu4">NOTEBOOK</a></li> | ||
<li><a href="https://2010.igem.org/Team:Virginia_United/Safety" rel="ddsubmenu3">SAFETY</a></li> | <li><a href="https://2010.igem.org/Team:Virginia_United/Safety" rel="ddsubmenu3">SAFETY</a></li> | ||
+ | <li><a href="https://2010.igem.org/Team:Virginia_United/Safety" rel="ddsubmenu3">HUMAN PRACTICES</a></li> | ||
<li><a href="#sponsors">SPONSORS</a></li> | <li><a href="#sponsors">SPONSORS</a></li> | ||
<li><a href="#gallery"rel="ddsubmenu5">GALLERY</a></li> | <li><a href="#gallery"rel="ddsubmenu5">GALLERY</a></li> |
Revision as of 21:36, 10 October 2010
![Virginia United1.jpg](/wiki/images/1/10/Virginia_United1.jpg)
An Engineering Approach to an Environmental Biosensor for Multiple Fish ToxinsWe are using a co-design approach to construct a multiple-compound biosensor that detects heavy metals (arsenic, mercury, copper) in aquatic environments. This chart describes the device logic that will be implemented:
![]() This logic will be implemented in three different designs. One of the approaches utilizes the operator sites of regulatory promoters, hybridizing two promoters’ operator sites into a single co-sensing promoter. In order for the hybrid promoter to initiate transcription, two target metals that control the operator sites must be present. Since, the hybrid promoters are attached to a single fluorescent protein, the detection of both metals can be measured using fluorescence.
![]() The second approach utilizes fluorescent protein complementation. When a target metal is detected by a cell, it will transcribe a fragment of a fluorescent protein. Upon translation, the portions of the fluorescent proteins will bond together and fluoresce, reporting the presence of the two target metals.
![]() In the third approach, each of the metals directly induces the production of a corresponding fluorescent protein. Fluorescence spectroscopy is used to separate out the wavelengths of each fluorescent protein, which will indicate what compounds are present in the environment.
![]()
Sponsors
|