Team:Michigan/Pili Expression
From 2010.igem.org
(Difference between revisions)
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#Prepared O/N cultures of K12 and ΔFimB, both with the FimB plasmid. | #Prepared O/N cultures of K12 and ΔFimB, both with the FimB plasmid. | ||
#Created 5 tubes: LB, LB+ΔFimB (both uninduced and induced), LB+K12 (both uninduced and induced) | #Created 5 tubes: LB, LB+ΔFimB (both uninduced and induced), LB+K12 (both uninduced and induced) | ||
- | *Used 5 ml of cell culture due to low algae culture available | + | #*Used 5 ml of cell culture due to low algae culture available |
- | #Added 100 ul | + | #Added 100 ul arabinose during exponential phase |
#Added 5 ml algae to every tube | #Added 5 ml algae to every tube | ||
#Shake for 10 min | #Shake for 10 min | ||
#Take 200 ul samples and measure OD600. | #Take 200 ul samples and measure OD600. | ||
+ | |||
+ | [[Image:ODassay10_8.png|800px]] | ||
+ | |||
+ | This graph illustrates the results. | ||
[[#top|Back to top]] | [[#top|Back to top]] |
Revision as of 05:38, 10 October 2010