Team:Michigan/Pili Expression
From 2010.igem.org
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We are planning to run a more quantitative assay later this week. | We are planning to run a more quantitative assay later this week. | ||
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+ | ==10/7/2010== | ||
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+ | Ran flocculation assay. | ||
+ | #Prepared O/N cultures of K12 and ΔFimB, both with the FimB plasmid. | ||
+ | #Created 5 tubes: LB, LB+ΔFimB (both uninduced and induced), LB+K12 (both uninduced and induced) | ||
+ | *Used 5 ml of cell culture due to low algae culture available | ||
+ | #Added 100 ul ara during exponential phase | ||
+ | #Added 5 ml algae to every tube | ||
+ | #Shake for 10 min | ||
+ | #Take 200 ul samples and measure OD600. | ||
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[[#top|Back to top]] | [[#top|Back to top]] | ||
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Revision as of 05:27, 10 October 2010