Team:Michigan/Oil Sands
From 2010.igem.org
(Difference between revisions)
Chillywings (Talk | contribs) (→8/30/2010) |
Chillywings (Talk | contribs) (→8/26/2010) |
||
Line 967: | Line 967: | ||
''Marcus'' | ''Marcus'' | ||
- | Attempted to perform electroporation according to [[Media:Electroporation Protocol 2.pdf|Electroporation Protocol 2]] | + | Attempted to perform electroporation according to [[Media:Electroporation Protocol 2.pdf|Electroporation Protocol 2]] with parts: |
+ | *pSB1AT3- Amp/Tet high copy backbone with RFP expression insert | ||
+ | *BBa_K145279- Tet resistance and Tet-activated GFP insert on Amp backbone | ||
+ | *BBa_I13504- Promoterless RBS-GFP insert on Amp backbone | ||
+ | *B0034- RBS on Amp backbone | ||
+ | |||
+ | However, cells took too long to grow out because only 1 mL of overnight was used to inoculate the 100 mL fresh culture. OD600 was only 0.142 after 4.5 hours. | ||
Made 10 Amp and 10 LB plates. | Made 10 Amp and 10 LB plates. |
Revision as of 05:59, 8 October 2010