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Team:Yale/LabNotebook/Cu
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Generated the stock solutions for the Followed the [https://2010.igem.org/Team:Yale/Protocols/cuabsorbance#Copper-Bathocuproinedisulfonic_acid_Standard_Absorbance_Curve standard curve protocol]. | Generated the stock solutions for the Followed the [https://2010.igem.org/Team:Yale/Protocols/cuabsorbance#Copper-Bathocuproinedisulfonic_acid_Standard_Absorbance_Curve standard curve protocol]. | ||
| - | Made a 100 mM stock solution of BCS. | + | *Made a 100 mM stock solution of BCS. |
| - | Made two CuSO4 dilution series, in LB and MM, from 10 mM Cu to 10 pM | + | *Made two CuSO4 dilution series, in LB and MM, from 10 mM Cu to 10 pM. |
| - | + | ||
===Oct 07, 2010; Bacterial growth assays, Cu ppt=== | ===Oct 07, 2010; Bacterial growth assays, Cu ppt=== | ||
Revision as of 20:51, 7 October 2010
General description of goal of this part of the project.
Contents |
Oct 05, 2010; Making stock solutions
Generated the stock solutions for the Followed the standard curve protocol.
- Made a 100 mM stock solution of BCS.
- Made two CuSO4 dilution series, in LB and MM, from 10 mM Cu to 10 pM.
Oct 07, 2010; Bacterial growth assays, Cu ppt
| # | Sample ID | Date and Time | 1 (nm) | 1 (Abs) |
| 1 | o/n culture | 10/7/2010 11:16:10 AM | 600 | 0.854 |
| 2 | MM t0 | 10/7/2010 11:44:45 AM | 600 | 0.002 |
| 3 | LB t0 | 10/7/2010 11:31:06 AM | 600 | 0.089 |
| 4 | LB t1 | 10/7/2010 12:47:55 PM | 600 | 0.189 |
| 5 | MM t1 | 10/7/2010 12:45:13 PM | 600 | 0.002 |
| 6 | MM t2 | 10/7/2010 1:41:51 PM | 600 | 0.004 |
| 7 | LB t2 | 10/7/2010 1:43:38 PM | 600 | 0.450 |
| 8 | MM t3 | 10/7/2010 2:44:50 PM | 600 | 0.001 |
| 9 | LB t3 | 10/7/2010 2:46:09 PM | 600 | 0.761 |
| 10 | MM t4 | 10/7/2010 3:53:12 PM | 600 | 0.005 |
| 11 | LB t4 | 10/7/2010 3:54:36 PM | 600 | 1.064 |
Useful links
[http://meta.wikimedia.org/wiki/Help:Wikitext_examples Wiki formatting help]
[http://excel2wiki.net/ Excel table to Wiki format tool]
