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Team:Cambridge/References/ProjectBioluminescence/Experiments
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With sucessfully transformed cells from 1 compare emission when adding: | With sucessfully transformed cells from 1 compare emission when adding: | ||
| - | + | * D-luciferin | |
| - | + | * CHBT | |
| - | + | * CHBT + D-Cysteine | |
| - | + | * CHBT + L-Cysteine | |
| - | + | * (CHBT + L-Luciferin? if necessary) | |
| + | ==3. Luciferin Regenerating Enzyme== | ||
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Latest revision as of 12:18, 7 October 2010
Link dumps: Bioluminescence |
Firefly luciferases|
Lucferin recovery|
Light output|
Experiments|
Modelling
Bioluminescence: Experiments
Firefly
1. Test luminescence with the Luciferase from the registry
- Transform WT E.coli with the luciferase from the registry.
- Feed with D-luciferin, develop a method for measuring light output
- Variables - concentration of inducer (if luciferase promoter inducible), concentration of D-luciferin
- Controls - no luciferin, no inducer
2. Test use of CHBT as a substrate
With sucessfully transformed cells from 1 compare emission when adding:
- D-luciferin
- CHBT
- CHBT + D-Cysteine
- CHBT + L-Cysteine
- (CHBT + L-Luciferin? if necessary)
3. Luciferin Regenerating Enzyme
