Team:Cambridge/References/ProjectBioluminescence/Experiments

From 2010.igem.org

(Difference between revisions)
 
(11 intermediate revisions not shown)
Line 1: Line 1:
-
{{:Team:Cambridge/Templates/header}}
+
{{:Team:Cambridge/Templates/headerMinimalprototype}}
-
{{:Team:Cambridge/LumNavTemplate}}
+
{{:Team:Cambridge/Templates/RefBar}}
 +
{{:Team:Cambridge/Templates/headerbar|colour=#96d446|title=Bioluminescence: Experiments}}
-
=Experiments=
+
=Firefly=
-
 
+
-
==Firefly==
+
[[Image:LRE_cycle_annotated.png |650px]]
[[Image:LRE_cycle_annotated.png |650px]]
-
1. Test this part of the pathway with the Luciferase from the registry  
+
==1. Test luminescence with the Luciferase from the registry==
 +
 
 +
* Transform WT E.coli with the luciferase from the registry.
 +
* Feed with D-luciferin, develop a method for measuring light output
 +
* Variables - concentration of inducer (if luciferase promoter inducible), concentration of D-luciferin
 +
* Controls - no luciferin, no inducer
 +
 
 +
==2. Test use of CHBT as a substrate==
-
2. Test use of CHBT as a substrate
+
With sucessfully transformed cells from 1 compare emission when adding:
 +
* D-luciferin
 +
* CHBT
 +
* CHBT + D-Cysteine
 +
* CHBT + L-Cysteine
 +
* (CHBT + L-Luciferin? if necessary)
-
a) D-cysteine
+
==3. Luciferin Regenerating Enzyme==
-
b) L-cysteine via L-luciferin
+
<html>
 +
</div>
 +
</html>
 +
{{:Team:Cambridge/Templates/footerMinimal}}

Latest revision as of 12:18, 7 October 2010