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Team:Cambridge/References/ProjectBioluminescence/Experiments
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| - | {{:Team:Cambridge/Templates/ | + | {{:Team:Cambridge/Templates/headerMinimalprototype}} |
| - | {{:Team:Cambridge/ | + | {{:Team:Cambridge/Templates/RefBar}} |
| + | {{:Team:Cambridge/Templates/headerbar|colour=#96d446|title=Bioluminescence: Experiments}} | ||
| - | + | =Firefly= | |
| - | + | ||
| - | + | ||
[[Image:LRE_cycle_annotated.png |650px]] | [[Image:LRE_cycle_annotated.png |650px]] | ||
| - | + | ==1. Test luminescence with the Luciferase from the registry== | |
| + | |||
| + | * Transform WT E.coli with the luciferase from the registry. | ||
| + | * Feed with D-luciferin, develop a method for measuring light output | ||
| + | * Variables - concentration of inducer (if luciferase promoter inducible), concentration of D-luciferin | ||
| + | * Controls - no luciferin, no inducer | ||
| + | |||
| + | ==2. Test use of CHBT as a substrate== | ||
| + | |||
| + | With sucessfully transformed cells from 1 compare emission when adding: | ||
| + | * D-luciferin | ||
| + | * CHBT | ||
| + | * CHBT + D-Cysteine | ||
| + | * CHBT + L-Cysteine | ||
| + | * (CHBT + L-Luciferin? if necessary) | ||
| + | |||
| + | ==3. Luciferin Regenerating Enzyme== | ||
| - | + | <html> | |
| + | </div> | ||
| + | </html> | ||
| + | {{:Team:Cambridge/Templates/footerMinimal}} | ||
Latest revision as of 12:18, 7 October 2010
Link dumps: Bioluminescence |
Firefly luciferases|
Lucferin recovery|
Light output|
Experiments|
Modelling
Bioluminescence: Experiments
Firefly
1. Test luminescence with the Luciferase from the registry
- Transform WT E.coli with the luciferase from the registry.
- Feed with D-luciferin, develop a method for measuring light output
- Variables - concentration of inducer (if luciferase promoter inducible), concentration of D-luciferin
- Controls - no luciferin, no inducer
2. Test use of CHBT as a substrate
With sucessfully transformed cells from 1 compare emission when adding:
- D-luciferin
- CHBT
- CHBT + D-Cysteine
- CHBT + L-Cysteine
- (CHBT + L-Luciferin? if necessary)
3. Luciferin Regenerating Enzyme
