Talk:Team:IvyTech-South Bend/24 August 2010

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(New page: == 8/24/10 == Today we will be pouring new plates for streaking new transformed cells. 1) I located some 2% Ara/agur made by Greg M. on 4/10/10. 2) Place the 250 ml Elemyier flask in m...)
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Today we will be pouring new plates for streaking new transformed cells.
Today we will be pouring new plates for streaking new transformed cells.
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1) I located some 2% Ara/agur made by Greg M. on 4/10/10.
1) I located some 2% Ara/agur made by Greg M. on 4/10/10.
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2) Place the 250 ml Elemyier flask in microwave to melt set on beverage 4oz setting and allow to run.
2) Place the 250 ml Elemyier flask in microwave to melt set on beverage 4oz setting and allow to run.
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3) Locate protocol for adding amp. To the agur. from openwetware.org/wiki/ampicillin.
3) Locate protocol for adding amp. To the agur. from openwetware.org/wiki/ampicillin.
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4) Hit run on microwave 4oz. bev. Setting for a sec. time
4) Hit run on microwave 4oz. bev. Setting for a sec. time
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5) After melting agur we will add 1.16 mg/mL Ampicillin.
5) After melting agur we will add 1.16 mg/mL Ampicillin.
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6) We added .6088 g Amp. powder to 5 mL of DI water then micropitetted 250mL into 20 centrifuge tubes freezing 19 of them in the -80 C freezer.
6) We added .6088 g Amp. powder to 5 mL of DI water then micropitetted 250mL into 20 centrifuge tubes freezing 19 of them in the -80 C freezer.
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7) We added 250 mL to 250 mL LB agur when cooled then poured 16 plates labeling them LB/amp 8/24/10 J.H.
7) We added 250 mL to 250 mL LB agur when cooled then poured 16 plates labeling them LB/amp 8/24/10 J.H.
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8) Let cool then place into bag and into fridge.
8) Let cool then place into bag and into fridge.
--[[User:Rchamberlin|Rchamberlin]] 18:27, 30 September 2010 (UTC)
--[[User:Rchamberlin|Rchamberlin]] 18:27, 30 September 2010 (UTC)

Latest revision as of 18:56, 30 September 2010

8/24/10

Today we will be pouring new plates for streaking new transformed cells.


1) I located some 2% Ara/agur made by Greg M. on 4/10/10.

2) Place the 250 ml Elemyier flask in microwave to melt set on beverage 4oz setting and allow to run.

3) Locate protocol for adding amp. To the agur. from openwetware.org/wiki/ampicillin.

4) Hit run on microwave 4oz. bev. Setting for a sec. time

5) After melting agur we will add 1.16 mg/mL Ampicillin.

6) We added .6088 g Amp. powder to 5 mL of DI water then micropitetted 250mL into 20 centrifuge tubes freezing 19 of them in the -80 C freezer.

7) We added 250 mL to 250 mL LB agur when cooled then poured 16 plates labeling them LB/amp 8/24/10 J.H.

8) Let cool then place into bag and into fridge. --Rchamberlin 18:27, 30 September 2010 (UTC)

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